Team:Freiburg Bioware

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virus

Gene delivery using viral vectors holds great promise for the treatment of acquired and inherited diseases. The human Adeno-Associated Virus (AAV) is a small, non-pathogenic, single-stranded DNA virus gaining increasing attention being both versatile and effective. Taking current knowledge into account, we generated a recombinant, modularized, BioBrick-compatible AAV ‘Virus Construction Kit’. We provide parts for modified capsid proteins, targeting modules, tumor-specific promoters, and prodrug-activating enzymes as well as readily assembled vectors for gene delivery and production of non-replicative virus particles. The viral tropism is altered by N-terminal fusion or by loop replacement of the capsid proteins. Functionality of viruses constructed from our kit was demonstrated by fluorescent protein expression in infected cells and by prodrug-induced killing of tumor cells upon viral delivery of a thymidine kinase. Incorporating multiple layers of safety, we provide a general tool to the growing field of personalized medicine and demonstrate its use in tumor therapy.

Project Results

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Virus Construction Kit

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BioBricks

Modularisation and Modification of the viral tropismn and the effector function resulted in many different BioBricks.
Click here to explore them.

Biosafety

The aspect of biological safety was considered well and a risk assessment and security profile for the Adeno-associated Virus were created. To hear about this click here.

Team

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Sponsors

Uni Freiburg Bioss kuk.lab Roche Qiagen Zeiss Starlab agilent Fermentas Hiss peqlab Geneart Eppendorf Gilson GATC ATG Biozym Avidity

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