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Team:Newcastle/26 August 2010
From 2010.igem.org
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yneA
PCR (Repeat)
Aim
To repeat the PCR that we did yesterday using the correct rocF primers.
Materials and Protocol
Please refer to PCR.
PCR Purification
Aim
To remove unwanted primers, taq polymerase, buffer and salts to obtain pure DNA.
Materials and Protocol
Please refer to PCR purification.
Digestion
Aim
To digest the PCR products of pSB1C3 and yneA from PCR purification.
Materials and Protocol
Please refer to restriction digest.
Gel extraction
Aim
To purify the DNA of yneA and pSB1C3 by extracting the bands from the gel after running gel electrophoresis. Concentration of DNA is then checked with NanoDrop.
Materials and Protocol
Please refer to:
Results
When we look at the gel through the GelDoc, only pSB1C3 was successfully digested with EcoR1 and Pst1 while yneA did not show any bands.
Conclusion
We will repeat the protocol again on 31.08.10.
   
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