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Team:KIT-Kyoto/Notebook/Promoters
From 2010.igem.org
Revision as of 08:17, 27 October 2010 by
Kazuma
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Abstract
Introduction
Materials & Methods
Results & Discussion
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Protocol
Promoters
Design Note
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Home
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Notebook
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Promoters
First
Week1 (Aug 11-14)
Week2 (Aug 15-21)
Week3 (Aug 22-28)
Week4 (Aug 29-Sep 4)
Week5 (Sep 5-11)
Week6 (Sep 12-18)
Week7 (Sep 19-25)
Week8 (Sep 26-Oct 2)
Week9 (Oct 3-9)
Week10 (Oct 10-13)
First
Week1 (Aug 22-28)
Week2 (Aug 29-Sep4)
Week3 (Sep5-11)
Week4 (Sep12-18)
Week5 (Sep19-25)
Week6 (Sep26-Oct2)
Week7 (Oct3-9)
Week8 (Oct10-16)
Week9 (Oct17-23)
Promoters
>>return to Sept.29
thu
Contents
K121013
ahpC
sufA
tetR
K121013
n=1
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.373
0.310
0.290
0.293
0.328
0.272
0.317
0.338
0.372
0.3130
0.274
0.285
0.325
0.261
0.300
0.327
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.346
0.329
0.321
0.272
0.366
0.281
0.479
0.324
0.353
0.343
0.320
0.275
0.356
0.280
0.461
0.327
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.327
0.289
0.331
0.356
0.352
0.353
0.344
0.474
0.339
0.293
0.333
0.365
0.358
0.368
0.340
0.467
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.439
0.463
0.416
0.466
0.461
0.319
0.475
0.419
0.450
0.468
0.401
0.488
0.441
0.314
0.459
0.418
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.314
0.445
0.355
0.452
0.342
0.353
0.442
0.392
0.310
0.491
0.370
0.471
0.338
0.348
0.424
0.392
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.468
0.478
0.459
0.459
0.446
0.354
0.515
0.420
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.452
0.4480
0.453
0.510
0.414
0.456
0.416
0.435
0.434
0.434
0.521
0.400
0.421
0.428
0.402
0.423
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.439
0.458
0.450
0.417
0.510
0.248
0.401
0.428
0.427
0.430
0.426
0.413
0.477
0.248
0.401
0.432
K121013
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
0.373
0.311
0.281
0.289
0.326
0.266
0.307
0.333
20min
0.350
0.336
0.320
0.274
0.361
0.280
0.470
0.325
30min
0.333
0.291
0.332
0.361
0.355
0.361
0.342
0.471
40min
0.444
0.465
0.408
0.477
0.451
0.316
0.467
0.419
50min
0.312
0.468
0.363
0.462
0.340
0.351
0.433
0.392
60min
0.468
0.478
0.459
0.459
0.446
0.354
0.515
0.420
70min
0.443
0.441
0.487
0.455
0.417
0.442
0.409
0.429
80min
0.433
0.444
0.436
0.415
0.493
0.248
0.401
0.430
n=2
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.324
0.282
0.327
0.300
0.329
0.301
0.306
0.362
0.324
0.286
0.315
0.299
0.320
0.306
0.306
0.367
0.321
0.294
0.313
0.300
0.327
0.310
0.315
0.367
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.464
0.458
0.436
0.405
1.24
0.440
0.380
0.439
0.438
0.432
0.400
0.376
1.22
0.425
0.350
0.452
0.419
0.421
0.399
0.373
1.23
0.413
0.374
0.456
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.346
0.353
0.441
0.333
0.426
0.385
0.364
0.402
0.353
0.345
0.432
0.340
0.409
0.372
0.368
0.416
0.346
0.346
0.416
0.342
0.401
0.366
0.360
0.405
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.399
0.461
0.413
0.541
0.416
0.395
0.510
0.372
0.370
0.461
0.396
0.524
0.403
0.377
0.506
0.377
0.368
0.450
0.397
0.54
0.396
0.377
0.480
0.379
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.500
0.429
0.355
0.441
0.566
0.462
0.450
0.414
0.469
0.407
0.341
0.405
0.564
0.421
0.418
0.408
0.434
0.390
0.340
0.384
0.575
0.406
0.409
0.413
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.405
0.398
0.395
0.365
0.442
0.414
0.403
0.411
0.388
0.405
0.386
0.365
0.415
0.406
0.413
0.409
0.403
0.404
0.389
0.370
0.405
0.414
0.417
0.405
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.426
0.600
0.557
0.413
0.454
0.450
0.434
0.387
0.426
0.545
0.504
0.421
0.454
0.462
0.429
0.393
0.436
0.506
0.500
0.431
0.465
0.466
0.446
0.404
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.457
0.445
0.456
0.457
0.464
0.466
0.450
0.417
0.474
0.458
0.468
0.471
0.477
0.484
0.465
0.430
0.474
0.460
0.470
0.485
0.481
0.490
0.468
0.416
90min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.469
0.458
0.491
0.475
0.483
0.444
0.533
0.446
0.482
0.464
0.450
0.481
0.493
0.443
0.543
0.450
0.491
0.467
0.510
0.486
0.506
0.45
0.540
0.448
K121013
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
0.323
0.287
0.318
0.299
0.326
0.306
0.309
0.365
20min
0.440
0.437
0.412
0.385
1.23
0.426
0.368
0.449
30min
0.348
0.348
0.429
0.338
0.412
0.374
0.364
0.408
40min
0.468
0.409
0.345
0.410
0.568
0.430
0.425
0.412
50min
0.399
0.402
0.390
0.367
0.420
0.411
0.411
0.408
60min
0.429
0.550
0.520
0.422
0.457
0.459
0.436
0.395
70min
0.469
0.455
0.465
0.471
0.474
0.480
0.461
0.421
80min
0.481
0.463
0.483
0.481
0.494
0.446
0.539
0.448
n=3
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.674
0.662
0.684
0.663
0.723
0.646
0.667
0.682
0.675
0.667
0.679
0.667
0.723
0.655
0.659
0.698
0.679
0.662
0.682
0.661
0.731
0.661
0.647
0.693
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.715
0.704
0.712
0.705
0.695
0.696
0.768
1.06
0.715
0.690
0.698
0.706
0.694
0.695
0.753
1.04
0.714
0.708
0.705
0.699
0.709
0.701
0.736
1.04
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.809
0.770
0.664
0.704
0.759
0.686
0.844
0.726
0.792
0.760
0.659
0.715
0.759
0.692
0.806
0.717
0.811
0.775
0.681
0.711
0.758
0.699
0.816
0.716
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.768
0.729
0.713
0.704
0.800
0.790
0.776
0.742
0.766
0.726
0.703
0.710
0.796
0.788
0.769
0.750
0.772
0.731
0.707
0.717
0.802
0.785
0.773
0.759
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.722
0.729
0.755
0.784
0.814
0.842
0.838
0.918
0.733
0.732
0.759
0.786
0.820
0.848
0.843
0.930
0.740
0.735
0.767
0.793
0.825
0.846
0.838
0.936
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.901
0.885
0.981
0.866
0.834
0.888
0.844
0.968
0.908
0.897
0.988
0.873
0.819
0.895
0.835
0.963
0.914
0.898
0.985
0.887
0.843
0.881
0.833
0.956
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.942
0.985
0.967
0.917
0.914
0.910
0.970
0.932
0.941
0.972
0.955
0.900
0.911
0.897
0.940
0.931
0.942
0.966
0.957
0.900
0.903
0.879
0.953
0.941
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
1.04
1.01
0.994
1.02
1.03
1.01
0.995
1.17
1.02
1.01
0.987
1.01
0.969
0.995
0.974
1.14
1.03
1.02
1.01
1.00
0.976
0.999
0.979
1.14
90min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.979
1.01
1.05
1.02
1.04
0.989
0.933
1.12
0.997
1.01
1.06
1.01
0.995
1.01
0.951
1.11
0.982
1.01
1.07
1.04
1.02
1.00
0.949
1.10
K121013
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
0.676
0.663
0.682
0.664
0.726
0.654
0.658
0.691
20min
0.715
0.701
0.705
0.703
0.700
0.698
0.752
1.05
30min
0.804
0.768
0.668
0.710
0.759
0.693
0.822
0.720
40min
0.769
0.725
0.707
0.710
0.799
0.788
0.772
0.750
50min
0.732
0.732
0.760
0.787
0.820
0.845
0.840
0.928
60min
0.942
0.974
0.960
0.905
0.909
0.895
0.955
0.935
70min
1.03
1.02
0.997
1.01
0.990
1.00
0.983
1.15
80min
0.986
1.01
1.06
1.02
1.02
0.999
0.944
1.11
90min
0.986
1.01
1.06
1.03
1.02
0.999
0.944
1.11
ahpC
n=1
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
6.36
6.55
6.64
5.95
7.19
9.85
10.4
12.0
6.48
6.71
6.73
6.11
7.34
10.2
11.0
12.4
6.52
6.72
6.78
6.15
7.46
10.4
11.3
12.8
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
7.82
6.91
7.31
6.92
7.26
11.4
12.6
13.6
7.89
7.04
7.47
6.97
7.37
11.4
12.9
13.9
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
8.17
8.20
8.62
8.71
8.97
11.3
13.6
16.1
8.32
8.30
8.79
8.83
8.98
11.4
13.9
16.1
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
8.37
8.75
9.20
8.52
8.86
11.5
14.9
16.0
8.39
8.73
9.19
8.55
9.05
11.9
15.3
16.2
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10.3
10.5
11.5
11.1
11.9
11.1
16.6
19.8
10.5
10.8
11.8
11.5
12.3
11.7
17.2
20.2
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
11.9
13.3
13.0
13.3
13.8
16.0
19.4
22.5
12.1
13.7
13.3
13.5
14.0
16.3
19.8
23.1
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
16.7
16.0
15.8
15.7
18.0
21.2
24.2
25.0
17.2
16.6
16.4
16.0
18.5
21.5
24.9
25.2
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
21.2
27.0
22.2
23.4
23.5
25.6
28.9
31.3
22
27.7
22.8
24.1
24.1
25.7
29.1
31.6
ahpC
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
6.45
6.66
6.72
6.07
7.33
10.2
10.9
12.4
20min
7.86
6.98
7.39
6.94
7.32
11.4
12.8
13.7
30min
8.25
8.25
8.71
8.77
8.98
11.3
13.8
16.1
40min
8.38
8.74
9.20
8.53
8.95
11.7
15.1
16.1
50min
10.4
10.6
11.7
11.3
12.1
11.4
16.9
20.0
60min
12.0
13.5
13.2
13.4
13.9
16.1
19.6
22.8
70min
16.9
16.3
16.1
15.9
18.3
21.3
24.6
25.1
80min
16.9
16.3
16.1
15.9
18.3
21.3
24.6
25.1
n=2
10min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
7.33
7.58
7.41
7.47
8.27
8.71
10.8
10.7
7.32
7.53
7.30
7.35
8.26
8.49
11.3
11.6
7.34
7.52
7.28
7.29
8.26
8.48
11.5
11.8
20min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
8.05
8.17
8.03
8.19
9.05
10.7
11.4
13.2
8.19
8.27
8.19
8.54
9.21
10.9
11.6
13.5
8.34
8.35
8.33
8.57
9.31
11.1
11.8
13.8
30min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
8.70
8.65
8.79
8.76
10.7
11.1
12.4
14.5
8.89
8.76
8.97
8.84
10.7
10.9
12.5
14.6
8.96
8.94
9.10
8.91
10.8
11.1
12.9
15.0
40min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
9.36
9.20
8.83
9.08
10.5
12.7
13.2
15.8
9.60
9.38
8.96
9.20
10.6
12.8
3.44
15.9
9.69
9.40
9.12
9.32
10.8
12.8
13.6
15.9
50min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10.1
10.5
10.3
10.2
11.3
12.8
14.6
17.9
10.5
10.9
10.6
10.7
11.5
13.1
14.8
18.2
10.7
11.1
10.8
10.8
11.8
13.4
15.1
18.4
60min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
11.2
12.0
11.9
12.5
12.8
14.4
16.5
19.2
11.3
12.2
12.1
12.7
13.1
14.5
16.6
19.2
11.5
12.5
12.4
13.1
13.3
14.9
16.9
19.3
70min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
13.2
12.3
13.6
13.4
15.8
17.6
18.4
21.3
12.8
12.3
13.3
13.5
15.4
17.4
18.0
20.5
12.9
13.1
14.2
13.7
16.1
17.9
18.9
20.9
80min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
16.2
18.1
17.9
18.3
18.3
20.9
17.5
22.9
16.1
18.2
17.9
18.5
18.1
20.5
17.3
22.3
16.4
18.9
18.4
19.3
18.7
21.1
18.2
23.4
ahpC
H
2
O
2
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10min
7.33
7.54
7.33
7.37
8.26
8.56
11.2
11.4
20min
8.20
8.26
8.18
8.43
9.19
10.9
11.6
13.5
30min
8.85
8.78
8.95
8.84
10.7
11.0
12.6
14.7
40min
9.55
9.33
8.97
9.20
10.6
12.8
13.4
15.8
50min
10.4
10.8
10.6
10.6
11.5
13.1
14.8
18.2
60min
11.3
12.2
12.2
12.8
13.1
14.6
16.7
19.2
70min
13.0
12.5
13.7
13.5
15.8
17.6
18.4
20.9
80min
16.3
18.4
18.1
18.7
18.4
20.8
17.7
22.9
n=3
10min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
5.52
5.37
5.72
5.53
6.00
6.93
7.63
7.77
5.63
5.46
5.69
5.49
5.95
6.93
7.67
7.71
5.56
5.42
5.69
5.44
5.95
6.97
7.80
7.80
20min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
6.11
5.92
5.70
5.54
5.77
7.36
8.28
10.0
6.05
5.89
5.50
5.43
5.57
7.17
7.96
9.71
6.08
5.93
5.55
5.51
5.67
7.28
8.22
9.98
30min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
6.02
5.81
6.29
6.03
6.30
7.78
8.56
10.1
6.03
5.81
6.26
6.03
6.29
7.42
8.52
9.96
6.12
5.87
6.31
6.10
6.35
7.56
8.63
9.95
40min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
6.22
5.90
6.46
6.01
5.74
12.2
8.66
10.3
6.30
6.07
6.61
6.20
5.83
12.7
8.89
10.4
6.39
6.15
6.66
6.32
6.00
12.9
9.10
10.5
50min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
6.59
6.48
6.77
6.74
6.92
8.14
9.59
12.1
6.72
6.66
6.95
6.90
7.00
8.33
9.74
12.1
6.86
6.80
7.10
7.05
7.11
8.47
10.0
12.3
60min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
6.36
7.38
7.05
7.15
7.84
8.82
10.4
11.7
6.43
7.60
7.25
7.38
7.94
8.85
10.5
11.8
6.71
7.79
7.42
7.56
8.10
9.02
10.6
11.9
70min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
9.19
9.16
9.25
9.09
8.83
11.7
12.5
13.0
9.40
9.31
9.21
9.15
8.71
11.5
12.4
13.3
9.61
9.56
9.37
9.36
8.71
11.8
12.6
13.4
80min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
11.0
11.1
11.5
10.9
12.0
13.7
15.4
15.4
11.2
11.3
11.6
11.1
12.2
13.5
15.2
15.3
11.3
11.7
11.8
11.6
12.4
13.9
15.8
15.4
90min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
16.0
17.6
15.0
14.6
15.3
16.4
17.1
17.3
16.2
17.8
15.1
14.7
15.5
16.4
17.2
17.5
16.4
18.1
15.3
15.0
15.7
16.6
17.4
17.7
sufA
n=1
10min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
1.24
1.13
1.33
1.26
1.29
1.49
2.62
4.24
1.278
1.16
1.34
1.29
1.27
1.52
2.74
4.28
1.29
1.16
1.33
1.29
1.27
1.55
2.75
4.36
20min
1.34
1.27
1.39
1.32
1.40
1.53
3.07
5.20
1.34
1.25
1.38
1.32
1.43
1.56
3.08
5.09
30min
1.31
1.25
1.39
1.30
1.34
1.65
2.91
5.03
1.33
1.29
1.41
1.32
1.37
1.67
2.94
4.97
40min
1.40
1.41
1.39
1.36
1.42
1.62
3.07
5.31
1.38
1.38
1.37
1.34
1.39
1.64
3.17
5.27
50min
1.72
0.983
1.23
1.38
1.40
1.53
3.17
5.49
1.23
0.994
1.26
1.35
1.39
1.59
3.17
5.58
60min
1.37
1.50
1.62
1.23
1.58
1.90
3.40
5.85
1.43
1.58
1.69
1.27
1.61
1.92
3.49
5.92
70min
1.77
1.77
2.46
1.82
2.11
2.20
4.00
6.13
1.90
1.87
2.58
1.92
2.18
2.24
4.00
6.10
80min
2.81
2.76
2.86
2.84
1.80
3.20
5.44
3.32
3.07
3.01
2.17
3.01
1.91
3.29
5.52
3.26
sufA
H
2
O
2
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10min
1.27
1.15
1.33
1.28
1.28
1.52
2.70
4.29
20min
1.34
1.26
1.38
1.32
1.42
1.54
3.07
5.15
30min
1.32
1.27
1.40
1.31
1.35
1.66
2.92
5.00
40min
1.39
1.39
1.38
1.35
1.40
1.63
3.12
5.29
50min
1.47
0.989
1.24
1.36
1.40
1.56
3.17
5.53
60min
1.40
1.54
1.65
1.25
1.60
1.91
3.45
5.89
70min
1.84
1.82
2.52
1.87
2.14
2.22
3.99
6.11
80min
2.94
2.89
2.51
2.93
1.86
3.24
5.48
3.29
n=2
10min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
3.03
3.12
3.16
2.96
3.15
3.12
3.49
3.03
3.05
3.10
2.93
3.10
3.08
3.54
4.07
3.01
3.07
3.13
2.95
3.11
3.06
3.46
4.07
20min
3.11
3.10
2.94
2.99
3.48
2.95
3.67
4.41
3.07
3.06
2.91
2.57
3.46
3.00
3.65
4.52
3.03
3.10
2.94
3.05
3.46
2.98
3.89
4.43
30min
3.30
1.93
3.28
3.00
3.19
3.18
3.80
4.57
3.21
1.81
3.16
2.98
3.15
3.15
3.74
4.59
3.17
1.90
3.16
3.03
3.22
3.17
3.80
4.67
40min
3.09
3.15
2.97
2.82
3.14
3.12
3.48
4.53
3.10
3.26
3.00
2.88
3.15
3.14
3.60
4.51
3.07
3.26
3.01
2.90
3.14
3.17
3.63
4.54
50min
3.15
3.06
3.06
3.10
3.14
3.37
3.78
4.84
3.11
3.11
3.13
3.18
3.25
3.39
3.86
4.84
3.11
3.10
3.09
3.14
3.22
3.37
3.82
4.83
60min
3.07
3.11
3.07
3.38
3.21
3.31
3.69
4.84
3.00
3.10
3.05
3.41
3.27
3.39
3.75
4.93
3.01
3.14
3.07
3.45
3.26
3.38
3.73
5.01
70min
3.64
3.29
3.37
3.52
3.33
3.41
4.08
4.99
3.51
3.11
3.21
3.36
3.14
3.26
3.37
4.75
3.59
3.12
3.27
3.40
3.19
3.26
3.78
4.69
80min
3.79
3.28
4.29
3.07
3.27
3.39
4.04
4.81
3.77
3.29
4.28
3.03
3.22
3.39
4.01
4.71
3.76
3.30
4.27
3.04
3.26
3.45
3.99
4.82
sufA
H
2
O
2
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10min
3.02
3.08
3.13
2.95
3.12
3.09
3.50
4.11
20min
3.07
3.09
2.93
2.87
3.47
2.98
3.73
4.45
30min
3.23
1.88
3.21
3.01
3.18
3.17
3.78
4.61
40min
3.09
3.22
2.99
2.87
3.15
3.14
3.57
4.53
50min
3.12
3.09
3.09
3.14
3.20
3.38
3.82
4.84
60min
3.02
3.12
3.07
3.41
3.24
3.36
3.72
4.93
70min
3.58
3.17
3.28
3.43
3.22
3.31
3.74
4.81
80min
3.77
3.29
4.28
3.05
3.25
3.41
4.01
4.78
n=3
10min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
2.10
1.87
2.03
2.09
2.08
2.15
2.82
3.49
2.07
1.87
1.99
2.07
2.00
2.12
2.76
3.51
2.02
1.85
1.98
2.05
1.98
2.12
2.81
3.59
20min
2.29
2.62
2.24
2.07
2.03
2.15
2.83
4.25
2.24
2.00
2.20
2.08
2.01
2.14
2.86
4.19
2.25
2.02
2.19
2.09
2.02
2.16
2.88
4.29
30min
2.04
1.95
1.89
1.96
2.09
2.07
2.90
4.09
2.00
1.86
1.87
1.96
2.06
2.09
2.96
4.09
2.01
1.91
1.91
1.98
2.08
2.11
2.99
4.10
40min
1.95
1.93
2.16
2.04
2.06
2.10
2.70
4.33
1.94
1.87
2.17
2.06
2.08
2.15
2.81
4.34
1.95
1.88
2.21
2.09
2.07
2.18
2.84
4.36
50min
1.92
2.06
2.03
2.06
1.93
2.23
2.63
4.63
1.92
2.09
2.04
2.09
1.94
2.22
2.67
4.62
1.92
2.07
2.06
2.10
2.08
2.22
2.70
4.64
60min
1.83
1.96
2.09
2.03
2.02
2.26
2.75
4.65
1.84
1.99
2.12
2.05
2.03
2.25
2.80
4.65
1.87
2.00
2.12
2.08
2.02
2.27
2.77
4.67
70min
2.13
2.22
2.22
2.09
2.23
2.30
2.90
4.32
2.12
2.21
2.22
2.04
2.22
2.26
2.85
4.26
2.15
2.24
2.24
2.07
2.23
2.28
2.86
4.26
80min
2.16
2.20
2.25
1.97
2.24
2.29
2.87
4.45
2.15
2.21
2.23
1.94
2.23
2.28
2.84
4.37
2.17
2.22
2.27
1.97
2.25
2.27
2.85
4.36
90min
2.29
2.13
2.26
2.16
2.19
2.28
3.08
4.30
2.30
2.16
2.27
2.17
2.13
2.29
3.08
4.31
2.28
2.19
2.30
2.20
2.16
2.31
3.14
4.38
sufA
H
2
O
2
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10min
2.06
1.86
2.00
2.07
2.02
2.13
2.80
3.53
20min
2.26
2.21
2.21
2.08
2.02
2.15
2.86
4.24
30min
2.01
1.91
1.89
1.97
2.08
2.09
2.95
4.09
40min
1.95
1.89
2.18
2.07
2.07
2.14
2.78
4.34
50min
1.92
2.07
2.05
2.08
1.98
2.23
2.67
4.63
60min
1.85
1.98
2.11
2.05
2.02
2.26
2.77
4.65
70min
2.13
2.22
2.23
2.06
2.22
2.28
2.87
4.28
80min
2.16
2.21
2.25
1.96
2.24
2.28
2.85
4.39
90min
2.29
2.14
2.28
2.18
2.16
2.30
3.10
4.33
tetR
n=1
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
14.4
14.6
14.3
15.0
14.7
15.0
15.0
13.8
14.5
14.8
14.3
15.0
14.5
15.0
14.8
13.8
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
15.2
15.2
15.0
16.0
14.9
14.7
14.8
15.1
15.5
15.5
15.2
15.2
14.8
14.8
14.6
14.7
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
15.7
15.9
16.0
16.3
15.2
16.5
16.3
15.4
16.0
16.2
16.3
16.4
15.3
16.6
16.5
15.6
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
13.3
15.2
14.4
14.5
14.8
15.6
15.5
15.3
14.4
16.1
15.0
15.0
15.5
15.8
15.9
15.4
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
18.1
18.3
17.4
18.4
17.0
18.3
18.2
19.7
18.3
18.6
17.8
18.7
17.4
18.5
18.5
20.2
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
18.5
19.8
17.6
18.3
18.7
19.2
19.0
21.1
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
21.8
21.4
20.9
20.8
21.9
21.8
20.7
20.2
21.9
21.3
20.7
20.8
21.9
21.5
20.6
20.1
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
22.9
25.2
22.6
22.1
21.6
22.8
22.1
20.8
23.2
24.8
22.8
22.1
21.8
22.9
22.4
20.8
tetR
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
14.5
14.7
14.3
15.0
15.0
15.0
14.9
13.8
20min
15.4
15.4
15.1
15.6
14.8
14.7
14.7
14.9
30min
15.9
16.1
16.1
16.4
15.3
16.5
16.4
15.5
40min
13.8
15.6
14.7
14.7
15.1
15.7
15.7
15.3
50min
18.2
18.5
17.6
18.6
17.2
18.4
18.4
19.9
60min
18.5
19.8
17.6
18.3
18.7
19.2
19.0
21.1
70min
21.9
21.4
20.8
20.8
21.9
21.6
20.7
20.1
80min
23.0
25.0
22.7
22.1
21.7
22.8
22.3
20.8
n=2
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
26.2
26.8
26.2
26.4
21.8
25.3
25.5
24.3
26.2
26.,6
25.9
26.1
21.6
25.8
26.4
24.5
26.1
26.8
26.1
26.2
22.0
26.3
26.4
24.8
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
28.9
28.1
24.5
26.8
26.8
27.7
25.7
25.0
29.4
27.8
24.5
26.6
26.4
27.3
25.5
25.4
29.8
28.0
24.9
27.1
26.7
27.7
25.9
25.8
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
28.7
28.0
2,9.0
28.1
27.0
27.4
27.9
28.4
29.3
28.5
29.6
28.6
27.3
28.1
27.8
28.3
29.7
28.8
30.0
28.9
27.9
28.3
28.2
28.2
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
26.6
26.9
19.8
27.5
29.3
27.1
26.3
28.5
27.7
28.3
20.8
29.2
30.6
29.3
27.0
27.8
28.2
29.0
20.9
29.2
31.2
29.5
27.3
28.0
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
32.9
29.3
29.6
29.6
31.5
31.6
31.3
30.6
33.0
29.6
29.9
29.7
31.7
32.0
31.5
30.7
33.0
30.0
30.2
30.2
32.0
32.3
31.8
30.9
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
35.0
29.1
28.8
26.9
26.8
27.1
30.0
27.9
34.9
27.1
28.8
26.9
26.8
27.1
29.6
27.9
34.7
27.8
28.6
26.9
26.4
27.4
29.6
27.9
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
37.9
34.5
33.6
33.0
32.4
32.4
31.1
33.7
37.8
34.6
33.6
33.2
32.7
32.2
31.4
33.6
38.3
35.3
34.1
33.5
33.1
33.1
31.9
34.1
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
38.5
35.2
36.7
35.9
38.1
36.9
32.5
34.6
38.9
35.9
37.2
36.8
39.0
37.9
33.2
35.3
39.4
36.3
37.3
37.0
39.1
38.1
33.4
35.4
90min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
40.1
38.6
37.9
36.7
37.1
37.1
38.3
34.4
40.5
38.8
38.5
36.7
37.4
38.5
39.0
35.2
40.7
39.2
39.0
37.3
37.5
38.6
39.4
35.7
tetR
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
26.2
26.7
26.1
26.2
21.8
25.8
26.1
24.5
20min
29.4
27.9
24.6
26.8
26.6
27.6
25.7
25.4
30min
29.5
28.4
29.5
28.5
27.4
27.9
28.0
28.3
40min
33.0
29.6
29.9
29.8
31.7
32.0
31.5
30.7
50min
34.9
28.0
28.8
26.9
26.7
27.2
29.6
27.9
60min
38.0
34.8
33.8
33.2
32.7
32.5
31.5
33.8
70min
38.9
35.8
37.1
36.6
38.7
37.6
33.0
35.1
80min
40.4
38.9
38.4
36.9
37.3
38.2
38.9
35.1
n=3
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
28.3
30.6
28.5
27.8
29.6
33.6
31.8
24.1
28.9
30.3
28.1
27.6
29.6
33.5
32.0
24.3
28.9
30.3
28.0
27.4
29.5
33.5
32.1
24.2
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
33.5
30.2
30.2
31.2
33.2
31.4
31.3
31.2
33.4
30.0
29.9
30.8
32.9
31.0
31.3
31.2
33.4
30.4
29.9
30.9
32.8
31.3
31.5
31.2
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
33.5
34.0
34.8
25.8
30.3
35.7
31.0
32.1
33.3
33.9
34.8
25.6
30.6
35.4
31.2
32.0
33.2
34.1
34.9
26.1
30.6
36.3
31.6
32.0
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
30.9
34.0
36.1
34.2
34.1
31.5
31.6
32.9
31.0
34.0
36.5
34.5
34.8
31.4
32.4
33.2
31.5
34.4
37.1
35.4
35.4
32.4
33.1
33.7
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
34.2
35.3
36.0
35.6
35.0
35.6
37.3
36.4
34.6
35.8
36.6
36.5
35.6
36.3
38.0
36.8
35.1
36.1
37.0
36.9
36.0
36.8
38.3
37.0
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
37.4
35.5
33.0
37.0
38.2
34.7
36.7
34.1
37.6
36.0
33.3
38.0
39.0
35.2
37.3
34.6
38.3
36.5
33.7
38.0
39.5
35.6
37.9
35.2
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
41.5
42.8
40.0
40.7
39.4
37.9
37.2
36.7
41.6
42.3
40.2
40.7
40.3
38.6
38.0
37.4
41.8
42.5
40.1
40.9
40.5
39.1
38.4
37.7
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
41.4
39.8
43.4
42.8
41.9
42.3
43.2
39.6
41.6
39.9
43.9
43.0
42.5
43.4
43.1
39.6
41.9
40.2
44.3
43.3
42.9
44.0
43.6
39.9
90min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
41.8
39.7
40.2
39.2
39.9
38.9
37.0
35.7
41.9
39.7
40.0
38.8
39.6
38.3
36.7
36.0
41.9
39.7
40.3
38.8
39.8
38.6
37.1
35.9
tetR
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
28.7
30.4
28.2
27.6
29.5
33.5
31.9
24.2
20min
33.4
30.2
30.0
31.0
32.9
31.2
31.3
31.2
30min
33.3
34.0
34.8
25.8
30.5
35.8
31.3
32.0
40min
31.1
34.1
36.6
34.7
34.7
31.8
32.4
33.3
50min
34.7
35.7
36.5
36.3
35.5
36.3
37.9
36.7
60min
41.6
42.5
40.1
40.7
40.1
38.5
37.9
37.3
70min
41.6
40.0
43.9
43.0
42.4
43.3
43.3
39.7
80min
41.9
39.7
40.2
39.0
39.8
38.6
37.0
35.9
90min
41.9
39.7
40.2
39.0
39.8
38.6
37.0
35.9