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Team:KIT-Kyoto/Notebook/Promoters
From 2010.igem.org
Revision as of 15:21, 26 October 2010 by
Aya
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Abstract
Introduction
Materials & Methods
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Protocol
Promoters
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Home
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Notebook
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Promoters
First
Week1 (Aug 11-14)
Week2 (Aug 15-21)
Week3 (Aug 22-28)
Week4 (Aug 29-Sep 4)
Week5 (Sep 5-11)
Week6 (Sep 12-18)
Week7 (Sep 19-25)
Week8 (Sep 26-Oct 2)
Week9 (Oct 3-9)
Week10 (Oct 10-13)
First
Week1 (Aug 22-28)
Week2 (Aug 29-Sep4)
Week3 (Sep5-11)
Week4 (Sep12-18)
Week5 (Sep19-25)
Week6 (Sep26-Oct2)
Week7 (Oct3-9)
Week8 (Oct10-16)
Week9 (Oct17-23)
Promoters
>>return to Sept.29
thu
Contents
K121013
ahpC
sufA
tetR
Data
K121013
n=1
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.373
0.310
0.290
0.293
0.328
0.272
0.317
0.338
0.3720
0.3130
0.274
0.285
0.325
0.261
0.300
0.327
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.346
0.329
0.321
0.272
0.366
0.281
0.479
0.324
0.353
0.343
0.320
0.275
0.356
0.280
0.461
0.327
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.327
0.289
0.331
0.356
0.352
0.353
0.344
0.474
0.339
0.293
0.333
0.365
0.358
0.368
0.340
0.467
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.439
0.463
0.416
0.466
0.461
0.319
0.475
0.419
0.450
0.468
0.401
0.488
0.441
0.314
0.459
0.418
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.314
0.445
0.355
0.452
0.342
0.353
0.442
0.392
0.310
0.491
0.370
0.471
0.338
0.348
0.424
0.392
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.468
0.478
0.459
0.459
0.446
0.354
0.515
0.420
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.452
0.4480
0.453
0.510
0.414
0.456
0.416
0.435
0.434
0.434
0.521
0.400
0.421
0.428
0.402
0.423
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.439
0.458
0.450
0.417
0.510
0.248
0.401
0.428
0.427
0.430
0.426
0.413
0.477
0.248
0.401
0.432
K121013
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
0.373
0.311
0.281
0.289
0.326
0.266
0.307
0.333
20min
0.350
0.336
0.320
0.274
0.361
0.280
0.470
0.325
30min
0.333
0.291
0.332
0.361
0.355
0.361
0.342
0.471
40min
0.444
0.465
0.408
0.477
0.451
0.316
0.467
0.419
50min
0.312
0.468
0.363
0.462
0.340
0.351
0.433
0.392
60min
0.468
0.478
0.459
0.459
0.446
0.354
0.515
0.420
70min
0.443
0.441
0.487
0.455
0.417
0.442
0.409
0.429
80min
0.433
0.444
0.436
0.415
0.493
0.248
0.401
0.430
n=2
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.324
0.282
0.327
0.300
0.329
0.301
0.306
0.362
0.324
0.286
0.315
0.299
0.320
0.306
0.306
0.367
0.321
0.294
0.313
0.300
0.327
0.310
0.315
0.367
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.464
0.458
0.436
0.405
1.24
0.440
0.380
0.439
0.438
0.432
0.400
0.376
1.22
0.425
0.350
0.452
0.419
0.421
0.399
0.373
1.23
0.413
0.374
0.456
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.346
0.353
0.441
0.333
0.426
0.385
0.364
0.402
0.353
0.345
0.432
0.340
0.409
0.372
0.368
0.416
0.346
0.346
0.416
0.342
0.401
0.366
0.360
0.405
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.399
0.461
0.413
0.541
0.416
0.395
0.510
0.372
0.370
0.461
0.396
0.524
0.403
0.377
0.506
0.377
0.368
0.450
0.397
0.54
0.396
0.377
0.480
0.379
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.500
0.429
0.355
0.441
0.566
0.462
0.450
0.414
0.469
0.407
0.341
0.405
0.564
0.421
0.418
0.408
0.434
0.390
0.340
0.384
0.575
0.406
0.409
0.413
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.405
0.398
0.395
0.365
0.442
0.414
0.403
0.411
0.388
0.405
0.386
0.365
0.415
0.406
0.413
0.409
0.403
0.404
0.389
0.370
0.405
0.414
0.417
0.405
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.426
0.600
0.557
0.413
0.454
0.450
0.434
0.387
0.426
0.545
0.504
0.421
0.454
0.462
0.429
0.393
0.436
0.506
0.500
0.431
0.465
0.466
0.446
0.404
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.457
0.445
0.456
0.457
0.464
0.466
0.450
0.417
0.474
0.458
0.468
0.471
0.477
0.484
0.465
0.430
0.474
0.460
0.470
0.485
0.481
0.490
0.468
0.416
90min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.469
0.458
0.491
0.475
0.483
0.444
0.533
0.446
0.482
0.464
0.450
0.481
0.493
0.443
0.543
0.450
0.491
0.467
0.510
0.486
0.506
0.45
0.540
0.448
K121013
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
0.323
0.287
0.318
0.299
0.326
0.306
0.309
0.365
20min
0.440
0.437
0.412
0.385
1.23
0.426
0.368
0.449
30min
0.348
0.348
0.429
0.338
0.412
0.374
0.364
0.408
40min
0.468
0.409
0.345
0.410
0.568
0.430
0.425
0.412
50min
0.399
0.402
0.390
0.367
0.420
0.411
0.411
0.408
60min
0.429
0.550
0.520
0.422
0.457
0.459
0.436
0.395
70min
0.469
0.455
0.465
0.471
0.474
0.480
0.461
0.421
80min
0.481
0.463
0.483
0.481
0.494
0.446
0.539
0.448
n=3
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.674
0.662
0.684
0.663
0.723
0.646
0.667
0.682
0.675
0.667
0.679
0.667
0.723
0.655
0.659
0.698
0.679
0.662
0.682
0.661
0.731
0.661
0.647
0.693
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.715
0.704
0.712
0.705
0.695
0.696
0.768
1.06
0.715
0.690
0.698
0.706
0.694
0.695
0.753
1.04
0.714
0.708
0.705
0.699
0.709
0.701
0.736
1.04
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.809
0.770
0.664
0.704
0.759
0.686
0.844
0.726
0.792
0.760
0.659
0.715
0.759
0.692
0.806
0.717
0.811
0.775
0.681
0.711
0.758
0.699
0.816
0.716
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.768
0.729
0.713
0.704
0.800
0.790
0.776
0.742
0.766
0.726
0.703
0.710
0.796
0.788
0.769
0.750
0.772
0.731
0.707
0.717
0.802
0.785
0.773
0.759
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.722
0.729
0.755
0.784
0.814
0.842
0.838
0.918
0.733
0.732
0.759
0.786
0.820
0.848
0.843
0.930
0.740
0.735
0.767
0.793
0.825
0.846
0.838
0.936
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.901
0.885
0.981
0.866
0.834
0.888
0.844
0.968
0.908
0.897
0.988
0.873
0.819
0.895
0.835
0.963
0.914
0.898
0.985
0.887
0.843
0.881
0.833
0.956
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.942
0.985
0.967
0.917
0.914
0.910
0.970
0.932
0.941
0.972
0.955
0.900
0.911
0.897
0.940
0.931
0.942
0.966
0.957
0.900
0.903
0.879
0.953
0.941
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
1.04
1.01
0.994
1.02
1.03
1.01
0.995
1.17
1.02
1.01
0.987
1.01
0.969
0.995
0.974
1.14
1.03
1.02
1.01
1.00
0.976
0.999
0.979
1.14
90min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.979
1.01
1.05
1.02
1.04
0.989
0.933
1.12
0.997
1.01
1.06
1.01
0.995
1.01
0.951
1.11
0.982
1.01
1.07
1.04
1.02
1.00
0.949
1.10
K121013
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
0.676
0.663
0.682
0.664
0.726
0.654
0.658
0.691
20min
0.715
0.701
0.705
0.703
0.700
0.698
0.752
1.05
30min
0.804
0.768
0.668
0.710
0.759
0.693
0.822
0.720
40min
0.769
0.725
0.707
0.710
0.799
0.788
0.772
0.750
50min
0.732
0.732
0.760
0.787
0.820
0.845
0.840
0.928
60min
0.942
0.974
0.960
0.905
0.909
0.895
0.955
0.935
70min
1.03
1.02
0.997
1.01
0.990
1.00
0.983
1.15
80min
0.986
1.01
1.06
1.02
1.02
0.999
0.944
1.11
90min
0.986
1.01
1.06
1.03
1.02
0.999
0.944
1.11
ahpC
n=1
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
6.36
6.55
6.64
5.95
7.19
9.85
10.4
12.0
6.48
6.71
6.73
6.11
7.34
10.2
11.0
12.4
6.52
6.72
6.78
6.15
7.46
10.4
11.3
12.8
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
7.82
6.91
7.31
6.92
7.26
11.4
12.6
13.6
7.89
7.04
7.47
6.97
7.37
11.4
12.9
13.9
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
8.17
8.20
8.62
8.71
8.97
11.3
13.6
16.1
8.32
8.30
8.79
8.83
8.98
11.4
13.9
16.1
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
8.37
8.75
9.20
8.52
8.86
11.5
14.9
16.0
8.39
8.73
9.19
8.55
9.05
11.9
15.3
16.2
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10.3
10.5
11.5
11.1
11.9
11.1
16.6
19.8
10.5
10.8
11.8
11.5
12.3
11.7
17.2
20.2
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
11.9
13.3
13.0
13.3
13.8
16.0
19.4
22.5
12.1
13.7
13.3
13.5
14.0
16.3
19.8
23.1
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
16.7
16.0
15.8
15.7
18.0
21.2
24.2
25.0
17.2
16.6
16.4
16.0
18.5
21.5
24.9
25.2
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
21.2
27.0
22.2
23.4
23.5
25.6
28.9
31.3
22
27.7
22.8
24.1
24.1
25.7
29.1
31.6
ahpC
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
6.45
6.66
6.72
6.07
7.33
10.2
10.9
12.4
20min
7.86
6.98
7.39
6.94
7.32
11.4
12.8
13.7
30min
8.25
8.25
8.71
8.77
8.98
11.3
13.8
16.1
40min
8.38
8.74
9.20
8.53
8.95
11.7
15.1
16.1
50min
10.4
10.6
11.7
11.3
12.1
11.4
16.9
20.0
60min
12.0
13.5
13.2
13.4
13.9
16.1
19.6
22.8
70min
16.9
16.3
16.1
15.9
18.3
21.3
24.6
25.1
80min
16.9
16.3
16.1
15.9
18.3
21.3
24.6
25.1
n=2
10min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
7.333
7.58
7.411
7.467
8.268
8.713
10.84
10.7
7.317
7.53
7.296
7.348
8.263
8.492
11.3
11.55
7.335
7.521
7.279
7.294
8.257
8.478
11.51
11.82
20min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
8.05
8.174
8.031
8.194
9.053
10.74
11.36
13.23
8.194
8.268
8.191
8.535
9.21
10.92
11.62
13.46
8.343
8.346
8.33
8.573
9.305
11.07
11.83
13.77
30min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
8.695
8.646
8.785
8.764
10.66
11.09
12.42
14.49
8.888
8.759
8.966
8.837
10.69
10.89
12.5
14.62
8.961
8.943
9.097
8.908
10.82
11.08
12.9
14.99
40min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
9.358
9.202
8.825
9.08
10.45
12.69
13.23
15.75
9.595
9.378
8.961
9.202
10.6
12.83
3.44
15.86
9.688
9.403
9.116
9.324
10.77
12.77
13.63
15.91
50min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10.13
10.52
10.3
10.19
11.25
12.81
14.59
17.94
10.49
10.86
10.63
10.65
11.54
13.09
14.83
18.18
10.69
11.09
10.84
10.81
11.81
13.36
15.08
18.36
60min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
11.16
11.96
11.93
12.54
12.82
14.43
16.53
19.15
11.26
12.23
12.11
12.71
13.05
14.48
16.62
19.21
11.54
12.45
12.41
13.07
13.3
14.92
16.91
19.32
70min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
13.23
12.29
13.61
13.44
15.82
17.61
18.35
21.25
12.76
12.25
13.28
13.46
15.42
17.38
18.01
20.47
12.91
13.05
14.17
13.74
16.12
17.89
18.87
20.93
80min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
16.23
18.13
17.86
18.3
18.33
20.91
17.46
22.93
16.11
18.24
17.9
18.53
18.05
20.51
17.34
22.31
16.44
18.88
18.4
19.29
18.69
21.1
18.15
23.41
ahpC
H
2
O
2
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10min
7.328333333
7.543666667
7.328666667
7.369666667
8.262666667
8.561
11.21666667
11.35666667
20min
8.195666667
8.262666667
8.184
8.434
9.189333333
10.91 11.60333333
13.48666667
30min
8.848
8.782666667
8.949333333
8.836333333
10.72333333
11.02
12.60666667
14.7
40min
9.547
9.327666667
8.967333333
9.202
10.60666667
12.76333333
13.43333333
15.84
50min
10.43666667
10.82333333
10.59
10.55
11.53333333
13.08666667
14.83333333
18.16
60min
11.32
12.21333333
12.15
12.77333333
13.05666667
14.61
16.68666667
19.22666667
70min
12.96666667
12.53
13.68666667
13.54666667
15.78666667
17.62666667
18.41
20.88333333
80min
16.26
18.41666667
18.05333333
18.70666667
18.35666667
20.84
17.65
22.88333333
n=3
10min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
5.519
5.366
5.722
5.526
6.003
6.93
7.826
7.772
5.628
5.463
5.693
5.491
5.948
6.931
7.672
7.709
5.555
5.419
5.69
5.439
5.948
6.97
7.796
7.799
20min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
6.114
5.922
5.68
5.54
5.765
7.362
8.28
10
6.045
5.887
5.504
5.428
5.571
7.169
7.957
9.709
6.076
5.928
5.551
5.51
5.665
7.278
8.215
9.976
30min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
6.02
5.805
6.293
6.025
6.297
7.475
8.558
10.12
6.027
5.805
6.261
6.028
6.286
7.421
8.522
9.961
6.116
5.871
6.314
6.102
6.349
7.561
8.628
9.952
40min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
6.217
5.9
6.459
6.009
5.743
12.2
8.663
10.28
6.302
6.069
6.606
6.202
5.831
12.71
8.892
10.38
6.392
6.145
6.663
6.317
6.002
12.94
9.101
10.52
50min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
6.59
6.478
6.767
6.743
6.917
8.139
9.588
12.06
6.724
6.662
6.953
6.901
7
8.334
9.738
12.09
6.859
6.801
7.103
7.051
7.109
8.47
10.04
12.34
60min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
6.356
7.38
7.051
7.147
7.835
8.82
10.36
11.71
6.433
7.603
7.245
7.381
7.938
8.852
10.47
11.75
6.707
7.79
7.424
7.559
8.102
9.022
10.63
11.9
70min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
9.194
9.162
9.247
9.085
8.83
11.65
12.54
12.95
9.395
9.308
9.208
9.154
8.706
11.53
12.44
13.27
9.605
9.564
9.367
9.358
8.712
11.78
12.62
13.42
80min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10.99
11.08
11.51
10.92
12.04
13.71
15.42
15.37
11.17
11.32
11.64
11.07
12.24
13.5
15.22
15.26
11.34
11.67
11.84
11.64
12.37
13.85
15.8
15.35
90min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
16.03
17.62
14.97
14.62
15.31
16.37
17.11
17.3
16.2
17.81
15.08
14.67
15.46
16.44
17.19
17.45
16.39
18.07
15.34
14.96
15.7
16.64
17.38
17.66
sufA
n=1
10min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
1.24
1.133
1.334
1.261
1.293
1.493
2.62
4.241
1.277
1.162
1.338
1.292
1.266
1.52
2.744
4.28
1.29
1.163
1.332
1.292
1.271
1.546
2.749
4.356
20min
1.336
1.274
1.385
1.32
1.4
1.533
3.065
5.204
1.341
1.253
1.382
1.318
1.432
1.556
3.076
5.088
30min
1.311
1.252
1.385
1.302
1.336
1.645
2.905
5.032
1.327
1.291
1.412
1.315
1.369
1.666
2.938
4.97
40min
1.4
1.412
1.391
1.362
1.417
1.62
3.066
5.306
1.382
1.377
1.372
1.337
1.385
1.641
3.165
5.274
50min
1.717
0.9834
1.227
1.377
1.399
1.529
3.173
5.49
1.23
0.9939
1.262
1.35
1.391
1.591
3.169
5.575
60min
1.369
1.503
1.617
1.232
1.579
1.898
3.4
5.852
1.433
1.58
1.686
1.268
1.612
1.923
3.49
5.921
70min
1.767
1.769
2.46
1.818
2.108
2.201
3.98
6.126
1.903
1.868
2.58
1.916
2.18
2.24
3.995
6.101
80min
2.805
2.757
2.855
2.837
1.796
3.2
5.443
3.323
3.072
3.013
2.169
3.014
1.914
3.289
5.524
3.264
sufA
H
2
O
2
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10min
1.269
1.152666667
1.334666667
1.281666667
1.276666667
1.519666667
2.704333333
4.292333333
20min
1.3385
1.2635
1.3835
1.319
1.416
1.5445
3.0705
5.146
30min
1.319
1.2715
1.3985
1.3085
1.3525
1.6555
2.9215
5.001
40min
1.391
1.3945
1.3815
1.3495
1.401
1.6305
3.1155
5.29
50min
1.4735
0.98865
1.2445
1.3635
1.395
1.56
3.171
5.5325
60min
1.401
1.5415
1.6515
1.25
1.5955
1.9105
3.445
5.8865
70min
1.835
1.8185
2.52
1.867
2.144
2.2205
3.9875
6.1135
80min
2.9385
2.885
2.512
2.9255
1.855
3.2445
5.4835
3.2935
n=2
10min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
3.029
3.117
3.163
2.962
3.152
3.124
3.49
4.184
3.025
3.051
3.1
2.928
3.101
3.082
3.542
4.07
3.012
3.072
3.133
2.952
3.112
3.055
3.456
4.068
20min
3.112
3.101
2.943
2.993
3.484
2.953
3.665
4.411
3.071
3.061
2.913
2.566
3.464
2.995
3.645
4.515
3.033
3.095
2.935
3.048
3.464
2.979
3.887
4.426
30min
3.303
1.933
3.283
3.004
3.189
3.178
3.798
4.565
3.211
1.811
3.161
2.979
3.146
3.153
3.739
4.593
3.173
1.897
3.164
3.033
3.215
3.17
3.804
4.667
40min
3.094
3.149
2.97
2.821
3.141
3.115
3.476
4.53
3.101
3.257
3.004
2.879
3.153
3.14
3.598
4.508
3.073
3.257
3.005
2.904
3.144
3.17
3.634
4.541
50min
3.146
3.058
3.06
3.102
3.143
3.374
3.78
4.835
3.108
3.109
3.13
3.175
3.246
3.39
3.857
4.843
3.11
3.1
3.086
3.141
3.217
3.372
3.822
4.828
60min
3.065
3.11
3.073
3.377
3.205
3.313
3.686
4.837
2.998
3.102
3.053
3.408
3.269
3.391
3.752
4.932
3.006
3.139
3.073
3.448
3.258
3.384
3.733
5.012
70min
3.639
3.29
3.365
3.522
3.333
3.41
4.082
4.99
3.512
3.112
3.207
3.355
3.139
3.263
3.371
4.749
3.591
3.118
3.27
3.402
3.187
3.256
3.779
4.69
80min
3.789
3.277
4.289
3.066
3.265
3.393
4.044
4.808
3.765
3.29
4.276
3.034
3.216
3.39
4.005
4.709
3.763
3.301
4.268
3.04
3.259
3.447
3.992
4.822
sufA
H
2
O
2
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10min
3.022
3.08
3.132
2.947333333
3.121666667
3.087
3.496
4.107333333
20min
3.072
3.085666667
2.930333333
2.869
3.470666667
2.975666667
3.732333333
4.450666667
30min
3.229
1.880333333
3.202666667
3.005333333
3.183333333
3.167
3.780333333
4.608333333
40min
3.089333333
3.221
2.993
2.868
3.146
3.141666667
3.569333333
4.526333333
50min
3.121333333
3.089
3.092
3.139333333
3.202
3.378666667
3.819666667
4.835333333
60min
3.023
3.117
3.066333333
3.411
3.244
3.362666667
3.723666667
4.927
70min
3.580666667
3.173333333
3.280666667
3.426333333
3.219666667
3.309666667
3.744
4.809666667
80min
3.772333333
3.289333333
4.277666667
3.046666667
3.246666667
3.41
4.013666667
4.779666667
n=3
10min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
2.098
1.873
2.032
2.088
2.075
2.148
2.824
3.488
2.072
1.865
1.991
2.065
2.002
2.12
2.763
3.51
2.018
1.846
1.978
2.049
1.984
2.117
2.809
3.594
20min
2.285
2.617
2.238
2.074
2.031
2.147
2.831
4.248
2.237
1.997
2.2
2.078
2.006
2.144
2.855
4.192
2.249
2.018
2.185
2.094
2.022
2.156
2.882
4.287
30min
2.037
1.945
1.885
1.96
2.088
2.072
2.897
4.085
1.995
1.863
1.868
1.964
2.058
2.089
2.956
4.088
2.009
1.906
1.91
1.981
2.078
2.109
2.994
4.095
40min
1.951
1.925
2.155
2.037
2.061
2.104
2.7
4.329
1.942
1.869
2.167
2.064
2.078
2.148
2.81
4.342
1.946
1.882
2.205
2.094
2.065
2.177
2.841
4.357
50min
1.918
2.057
2.034
2.055
1.927
2.23
2.633
4.63
1.923
2.087
2.043
2.087
1.938
2.223
2.665
4.624
1.923
2.07
2.059
2.098
2.084
2.224
2.698
4.639
60min
1.831
1.956
2.092
2.033
2.021
2.256
2.752
4.651
1.844
1.991
2.115
2.045
2.029
2.249
2.798
4.646
1.874
1.997
2.123
2.08
2.02
2.268
2.769
4.666
70min
2.13
2.218
2.218
2.089
2.227
2.301
2.901
4.32
2.123
2.21
2.22
2.036
2.219
2.255
2.849
4.258
2.147
2.238
2.242
2.065
2.225
2.278
2.857
4.261
80min
2.157
2.197
2.246
1.966
2.239
2.289
2.867
4.446
2.149
2.211
2.225
1.941
2.228
2.277
2.839
4.367
2.171
2.217
2.269
1.974
2.251
2.267
2.853
4.361
90min
2.285
2.127
2.26
2.159
2.186
2.283
3.076
4.301
2.302
2.161
2.271
2.174
2.13
2.29
3.08
4.311
2.284
2.185
2.299
2.2
2.162
2.313
3.137
4.384
sufA
H
2
O
2
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10min
2.062666667
1.861333333
2.000333333
2.067333333
2.020333333
2.128333333
2.798666667
3.530666667
20min
2.257
2.210666667
2.207666667
2.082
2.019666667
2.149
2.856
4.242333333
30min
2.013666667
1.904666667
1.887666667
1.968333333
2.074666667
2.09
2.949
4.089333333
40min
1.946333333
1.892
2.175666667
2.065
2.068
2.143
2.783666667
4.342666667
50min
1.921333333
2.071333333
2.045333333
2.08
1.983
2.225666667
2.665333333
4.631
60min
1.849666667
1.981333333
2.11
2.052666667
2.023333333
2.257666667
2.773
4.654333333
70min
2.133333333
2.222
2.226666667
2.063333333
2.223666667
2.278
2.869
4.279666667
80min
2.159
2.208333333
2.246666667
1.960333333
2.239333333
2.277666667
2.853
4.391333333
90min
2.290333333
2.144
2.276666667
2.177666667
2.159333333
2.295333333
3.097666667
4.332
tetR
n=1
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
14.4
14.63
14.28
15.01
14.66
14.97
14.96
13.84
14.51
14.76
14.3
15.01
14.52
15.04
14.78
13.82
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
15.19
15.24
15.03
15.99
14.85
14.7
14.79
15.09
15.5
15.46
15.21
15.17
14.78
14.75
14.57
14.71
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
15.67
15.94
15.99
16.32
15.23
16.5
16.34
15.43
16.02
16.21
16.29
16.41
15.34
16.57
16.47
15.64
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
13.32
15.19
14.39
14.48
14.82
15.58
15.51
15.26
14.35
16.09
15.03
14.95
15.47
15.8
15.88
15.42
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
18.08
18.31
17.4
18.41
17.03
18.25
18.18
19.67
18.26
18.61
17.78
18.72
17.4
18.51
18.53
20.16
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
18.46
19.82
17.61
18.28
18.69
19.21
18.96
21.1
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
21.83
21.43
20.92
20.81
21.89
21.8
20.7
20.2
21.93
21.27
20.72
20.76
21.9
21.49
20.62
20.09
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
22.89
25.21
22.64
22.08
21.57
22.78
22.14
20.79
23.18
24.76
22.76
22.09
21.79
22.85
22.4
20.84
tetR
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
14.455
14.695
14.29
15.01
14.59
15.005
14.87
13.83
20min
15.345
15.35
15.12
15.58
14.815
14.725
14.68
14.9
30min
15.845
16.075
16.14
16.365
15.285
16.535
16.405
15.535
40min
13.835
15.64
14.71
14.715
15.145
15.69
15.695
15.34
50min
18.17
18.46
17.59
18.565
17.215
18.38
18.355
19.915
60min
18.46
19.82
17.61
18.28
18.69
19.21
18.96
21.1
70min
21.88
21.35
20.82
20.785
21.895
21.645
20.66
20.145
80min
23.035
24.985
22.7
22.085
21.68
22.815
22.27
20.815