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Contents 1 yneA 1.1 Aims 1.2 Materials and Protocol 1.3 Results 1.3.1 Nanodrop results

yneA

Aims

Today we aim to extract yneA in the biobrick comapatible vector from yesterday's overnight culture. We aim to check the concentration from the miniprep with the nanodrop then digest the plasmid with EcoR1 and Nhe1, ligate into pGFPrrnB and transform cells to grow on a plate overnight.

Materials and Protocol

Please refer to protocols mentioned below for materials required:

• Plasmid extraction protocol
• Nanodrop protocol
• Restriction digest protocol
• Gel electrophoresis protocol
• Gel extraction protocol

Results

Nanodrop results

	yneA 1	yneA 2	yneA 3	yneA 4
Concentration of DNA ng/µl	247.3	233.6	456.6	140.0

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