Team:British Columbia/Notebook Biofilm
From 2010.igem.org
Protocol
Day 1
1. PIck an isolated colony from a Trypticase Soy Agar (TSA) plate and incubate in 5 mL of Trypticase Soy Broth (TSB) without shaking overnight.
Day 2
1. Vortex the overnight culture and dilute 30 uL of culture by 1:100 in a 1% glucose solution diluted with TSB. 2. akjhskjh