Team:British Columbia/Notebook Biofilm

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Protocol

Day 1

1. PIck an isolated colony from a Trypticase Soy Agar (TSA) plate and incubate in 5 mL of Trypticase Soy Broth (TSB) without shaking overnight.

Day 2

1. Vortex the overnight culture and dilute 30 uL of culture by 1:100 in a 1% glucose solution diluted with TSB. 2. akjhskjh