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Team:KIT-Kyoto/Notebook/Promoters
From 2010.igem.org
Revision as of 12:41, 26 October 2010 by
Matsubara3
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Our Team
Twitter
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Abstract
Introduction
Materials & Methods
Results & Discussion
References
Lab Note
Protocol
Promoters
Design Note
Photograph
Home
>
Notebook
>
Promoters
First
Week1 (Aug 11-14)
Week2 (Aug 15-21)
Week3 (Aug 22-28)
Week4 (Aug 29-Sep 4)
Week5 (Sep 5-11)
Week6 (Sep 12-18)
Week7 (Sep 19-25)
Week8 (Sep 26-Oct 2)
Week9 (Oct 3-9)
Week10 (Oct 10-13)
First
Week1 (Aug 22-28)
Week2 (Aug 29-Sep4)
Week3 (Sep5-11)
Week4 (Sep12-18)
Week5 (Sep19-25)
Week6 (Sep26-Oct2)
Week7 (Oct3-9)
Week8 (Oct10-16)
Week9 (Oct17-23)
各種プロモーター活性
>>retuen to Sept.29
th
Contents
K121013
ahpC
sufA
tetR
Data
K121013
n=1
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.373
0.310
0.290
0.293
0.328
0.272
0.317
0.338
0.3720
0.3130
0.274
0.285
0.325
0.261
0.300
0.327
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.346
0.329
0.321
0.272
0.366
0.281
0.479
0.324
0.353
0.343
0.320
0.275
0.356
0.280
0.461
0.327
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.327
0.289
0.331
0.356
0.352
0.353
0.344
0.474
0.339
0.293
0.333
0.365
0.358
0.368
0.340
0.467
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.439
0.463
0.416
0.466
0.461
0.319
0.475
0.419
0.450
0.468
0.401
0.488
0.441
0.314
0.459
0.418
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.314
0.445
0.355
0.452
0.342
0.353
0.442
0.392
0.310
0.491
0.370
0.471
0.338
0.348
0.424
0.392
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.468
0.478
0.459
0.459
0.446
0.354
0.515
0.420
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.452
0.4480
0.453
0.510
0.414
0.456
0.416
0.435
0.434
0.434
0.521
0.400
0.421
0.428
0.402
0.423
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.439
0.458
0.450
0.417
0.510
0.248
0.401
0.428
0.427
0.430
0.426
0.413
0.477
0.248
0.401
0.432
K121013
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
0.373
0.311
0.281
0.289
0.326
0.266
0.307
0.333
20min
0.350
0.336
0.320
0.274
0.361
0.280
0.470
0.325
30min
0.333
0.291
0.332
0.361
0.355
0.361
0.342
0.471
40min
0.444
0.465
0.408
0.477
0.451
0.316
0.467
0.419
50min
0.312
0.468
0.363
0.462
0.340
0.351
0.433
0.392
60min
0.468
0.478
0.459
0.459
0.446
0.354
0.515
0.420
70min
0.443
0.441
0.487
0.455
0.417
0.442
0.409
0.429
80min
0.433
0.444
0.436
0.415
0.493
0.248
0.401
0.430
n=2
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.324
0.282
0.327
0.300
0.329
0.301
0.306
0.362
0.324
0.286
0.315
0.299
0.320
0.306
0.306
0.367
0.321
0.294
0.313
0.300
0.327
0.310
0.315
0.367
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.464
0.458
0.436
0.405
1.24
0.440
0.380
0.439
0.438
0.432
0.400
0.376
1.22
0.425
0.350
0.452
0.419
0.421
0.399
0.373
1.23
0.413
0.374
0.456
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.346
0.353
0.441
0.333
0.426
0.385
0.364
0.402
0.353
0.345
0.432
0.340
0.409
0.372
0.368
0.416
0.346
0.346
0.416
0.342
0.401
0.366
0.360
0.405
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.399
0.461
0.413
0.541
0.416
0.395
0.510
0.372
0.370
0.461
0.396
0.524
0.403
0.377
0.506
0.377
0.368
0.450
0.397
0.54
0.396
0.377
0.480
0.379
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.500
0.429
0.355
0.441
0.566
0.462
0.450
0.414
0.469
0.407
0.341
0.405
0.564
0.421
0.418
0.408
0.434
0.390
0.340
0.384
0.575
0.406
0.409
0.413
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.405
0.398
0.395
0.365
0.442
0.414
0.403
0.411
0.388
0.405
0.386
0.365
0.415
0.406
0.413
0.409
0.403
0.404
0.389
0.370
0.405
0.414
0.417
0.405
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.426
0.600
0.557
0.413
0.454
0.450
0.434
0.387
0.426
0.545
0.504
0.421
0.454
0.462
0.429
0.393
0.436
0.506
0.500
0.431
0.465
0.466
0.446
0.404
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.457
0.445
0.456
0.457
0.464
0.466
0.450
0.417
0.474
0.458
0.468
0.471
0.477
0.484
0.465
0.430
0.474
0.460
0.470
0.485
0.481
0.490
0.468
0.416
90min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.469
0.458
0.491
0.475
0.483
0.444
0.533
0.446
0.482
0.464
0.450
0.481
0.493
0.443
0.543
0.450
0.491
0.467
0.510
0.486
0.506
0.45
0.540
0.448
K121013
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
0.323
0.287
0.318
0.299
0.326
0.306
0.309
0.365
20min
0.440
0.437
0.412
0.385
1.23
0.426
0.368
0.449
30min
0.348
0.348
0.429
0.338
0.412
0.374
0.364
0.408
40min
0.468
0.409
0.345
0.410
0.568
0.430
0.425
0.412
50min
0.399
0.402
0.390
0.367
0.420
0.411
0.411
0.408
60min
0.429
0.550
0.520
0.422
0.457
0.459
0.436
0.395
70min
0.469
0.455
0.465
0.471
0.474
0.480
0.461
0.421
80min
0.481
0.463
0.483
0.481
0.494
0.446
0.539
0.448
n=3
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.674
0.662
0.684
0.663
0.723
0.646
0.667
0.682
0.675
0.667
0.679
0.667
0.723
0.655
0.659
0.698
0.679
0.662
0.682
0.661
0.731
0.661
0.647
0.693
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.715
0.704
0.712
0.705
0.695
0.696
0.768
1.06
0.715
0.690
0.698
0.706
0.694
0.695
0.753
1.04
0.714
0.708
0.705
0.699
0.709
0.701
0.736
1.04
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.809
0.770
0.664
0.704
0.759
0.686
0.844
0.726
0.792
0.760
0.659
0.715
0.759
0.692
0.806
0.717
0.811
0.775
0.681
0.711
0.758
0.699
0.816
0.716
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.768
0.729
0.713
0.704
0.800
0.790
0.776
0.742
0.766
0.726
0.703
0.710
0.796
0.788
0.769
0.750
0.772
0.731
0.707
0.717
0.802
0.785
0.773
0.759
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.722
0.729
0.755
0.784
0.814
0.842
0.838
0.918
0.733
0.732
0.759
0.786
0.820
0.848
0.843
0.930
0.740
0.735
0.767
0.793
0.825
0.846
0.838
0.936
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.901
0.885
0.981
0.866
0.834
0.888
0.844
0.968
0.908
0.897
0.988
0.873
0.819
0.895
0.835
0.963
0.914
0.898
0.985
0.887
0.843
0.881
0.833
0.956
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.942
0.985
0.967
0.917
0.914
0.910
0.970
0.932
0.941
0.972
0.955
0.900
0.911
0.897
0.940
0.931
0.942
0.966
0.957
0.900
0.903
0.879
0.953
0.941
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
1.04
1.01
0.994
1.02
1.03
1.01
0.995
1.17
1.02
1.01
0.987
1.01
0.969
0.995
0.974
1.14
1.03
1.02
1.01
1.00
0.976
0.999
0.979
1.14
90min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.979
1.01
1.05
1.02
1.04
0.989
0.933
1.12
0.997
1.01
1.06
1.01
0.995
1.01
0.951
1.11
0.982
1.01
1.07
1.04
1.02
1.00
0.949
1.10
K121013
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
0.676
0.663
0.682
0.664
0.726
0.654
0.658
0.691
20min
0.715
0.701
0.705
0.703
0.700
0.698
0.752
1.05
30min
0.804
0.768
0.668
0.710
0.759
0.693
0.822
0.720
40min
0.769
0.725
0.707
0.710
0.799
0.788
0.772
0.750
50min
0.732
0.732
0.760
0.787
0.820
0.845
0.840
0.928
60min
0.942
0.974
0.960
0.905
0.909
0.895
0.955
0.935
70min
1.03
1.02
0.997
1.01
0.990
1.00
0.983
1.15
80min
0.986
1.01
1.06
1.02
1.02
0.999
0.944
1.11
90min
0.986
1.01
1.06
1.03
1.02
0.999
0.944
1.11
ahpC
n=1
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
6.36
6.55
6.64
5.95
7.19
9.85
10.4
12.0
6.48
6.71
6.73
6.11
7.34
10.2
11.0
12.4
6.52
6.72
6.78
6.15
7.46
10.4
11.3
12.8
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
7.82
6.91
7.31
6.92
7.26
11.4
12.6
13.6
7.89
7.04
7.47
6.97
7.37
11.4
12.9
13.9
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
8.17
8.20
8.62
8.71
8.97
11.3
13.6
16.1
8.32
8.30
8.79
8.83
8.98
11.4
13.9
16.1
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
8.37
8.75
9.20
8.52
8.86
11.5
14.9
16.0
8.39
8.73
9.19
8.55
9.05
11.9
15.3
16.2
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10.3
10.5
11.5
11.1
11.9
11.1
16.6
19.8
10.5
10.8
11.8
11.5
12.3
11.7
17.2
20.2
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
11.9
13.3
13.0
13.3
13.8
16.0
19.4
22.5
12.1
13.7
13.3
13.5
14.0
16.3
19.8
23.1
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
16.7
16.0
15.8
15.7
18.0
21.2
24.2
25.0
17.2
16.6
16.4
16.0
18.5
21.5
24.9
25.2
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
21.2
27.0
22.2
23.4
23.5
25.6
28.9
31.3
22
27.7
22.8
24.1
24.1
25.7
29.1
31.6
ahpC
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
6.45
6.66
6.72
6.07
7.33
10.2
10.9
12.4
20min
7.86
6.98
7.39
6.94
7.32
11.4
12.8
13.7
30min
8.25
8.25
8.71
8.77
8.98
11.3
13.8
16.1
40min
8.38
8.74
9.20
8.53
8.95
11.7
15.1
16.1
50min
10.4
10.6
11.7
11.3
12.1
11.4
16.9
20.0
60min
12.0
13.5
13.2
13.4
13.9
16.1
19.6
22.8
70min
16.9
16.3
16.1
15.9
18.3
21.3
24.6
25.1
80min
16.9
16.3
16.1
15.9
18.3
21.3
24.6
25.1
n=2
10min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
7.333
7.58
7.411
7.467
8.268
8.713
10.84
10.7
7.317
7.53
7.296
7.348
8.263
8.492
11.3
11.55
7.335
7.521
7.279
7.294
8.257
8.478
11.51
11.82
20min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
8.05
8.174
8.031
8.194
9.053
10.74
11.36
13.23
8.194
8.268
8.191
8.535
9.21
10.92
11.62
13.46
8.343
8.346
8.33
8.573
9.305
11.07
11.83
13.77
30min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
8.695
8.646
8.785
8.764
10.66
11.09
12.42
14.49
8.888
8.759
8.966
8.837
10.69
10.89
12.5
14.62
8.961
8.943
9.097
8.908
10.82
11.08
12.9
14.99
40min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
9.358
9.202
8.825
9.08
10.45
12.69
13.23
15.75
9.595
9.378
8.961
9.202
10.6
12.83
3.44
15.86
9.688
9.403
9.116
9.324
10.77
12.77
13.63
15.91
50min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10.13
10.52
10.3
10.19
11.25
12.81
14.59
17.94
10.49
10.86
10.63
10.65
11.54
13.09
14.83
18.18
10.69
11.09
10.84
10.81
11.81
13.36
15.08
18.36
60min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
11.16
11.96
11.93
12.54
12.82
14.43
16.53
19.15
11.26
12.23
12.11
12.71
13.05
14.48
16.62
19.21
11.54
12.45
12.41
13.07
13.3
14.92
16.91
19.32
70min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
13.23
12.29
13.61
13.44
15.82
17.61
18.35
21.25
12.76
12.25
13.28
13.46
15.42
17.38
18.01
20.47
12.91
13.05
14.17
13.74
16.12
17.89
18.87
20.93
80min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
16.23
18.13
17.86
18.3
18.33
20.91
17.46
22.93
16.11
18.24
17.9
18.53
18.05
20.51
17.34
22.31
16.44
18.88
18.4
19.29
18.69
21.1
18.15
23.41
ahpC
H
2
O
2
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10min
7.328333333
7.543666667
7.328666667
7.369666667
8.262666667
8.561
11.21666667
11.35666667
20min
8.195666667
8.262666667
8.184
8.434
9.189333333
10.91 11.60333333
13.48666667
30min
8.848
8.782666667
8.949333333
8.836333333
10.72333333
11.02
12.60666667
14.7
40min
9.547
9.327666667
8.967333333
9.202
10.60666667
12.76333333
13.43333333
15.84
50min
10.43666667
10.82333333
10.59
10.55
11.53333333
13.08666667
14.83333333
18.16
60min
11.32
12.21333333
12.15
12.77333333
13.05666667
14.61
16.68666667
19.22666667
70min
12.96666667
12.53
13.68666667
13.54666667
15.78666667
17.62666667
18.41
20.88333333
80min
16.26
18.41666667
18.05333333
18.70666667
18.35666667
20.84
17.65
22.88333333
sufA
tetR