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Team:KIT-Kyoto/Notebook/Promoters
From 2010.igem.org
Revision as of 01:56, 26 October 2010 by
Adachi
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Our Team
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Abstract
Introduction
Materials & Methods
Results & Discussion
References
Lab Note
Protocol
Promoters
Design Note
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Home
>
Notebook
>
Promoters
First
Week1 (Aug 11-14)
Week2 (Aug 15-21)
Week3 (Aug 22-28)
Week4 (Aug 29-Sep 4)
Week5 (Sep 5-11)
Week6 (Sep 12-18)
Week7 (Sep 19-25)
Week8 (Sep 26-Oct 2)
Week9 (Oct 3-9)
Week10 (Oct 10-13)
First
Week1 (Aug 22-28)
Week2 (Aug 29-Sep4)
Week3 (Sep5-11)
Week4 (Sep12-18)
Week5 (Sep19-25)
Week6 (Sep26-Oct2)
Week7 (Oct3-9)
Week8 (Oct10-16)
Week9 (Oct17-23)
各種プロモーター活性
>>retuen to Sept.29
th
Contents
K121013
ahpC
sufA
tetR
Data
K121013
n=1
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.3734
0.3082
0.2886
0.2934
0.3283
0.2716
0.3172
0.3384
0.3717
0.3127
0.274
0.2845
0.3245
0.2607
0.2967
0.3267
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.3463
0.3286
0.3206
0.272
0.3663
0.2803
0.4792
0.3242
0.3534
0.3433
0.3197
0.2754
0.3558
0.2799
0.4612
0.3265
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.3274
0.2893
0.331
0.3564
0.3519
0.353
0.3443
0.4742
0.3389
0.2932
0.3331
0.365
0.358
0.368
0.3399
0.4669
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.4385
0.4628
0.4159
0.4656
0.4612
0.3186
0.4748
0.4191
0.4502
0.4679
0.4007
0.488
0.4407
0.3139
0.4587
0.4181
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.314
0.4448
0.3551
0.4522
0.3417
0.3533
0.4415
0.3924
0.3091
0.4911
0.3698
0.4707
0.3383
0.348
0.4238
0.3919
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.4679
0.4767
0.4592
0.4591
0.4461
0.3544
0.5154
0.4195
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.4521
0.4479
0.4534
0.5095
0.4139
0.4564
0.4158
0.4354
0.4338
0.4338
0.5212
0.3995
0.4209
0.4283
0.4015
0.4227
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.4388
0.4584
0.446
0.417
0.5097
0.2479
0.4006
0.4278
0.4273
0.4302
0.4259
0.4129
0.4772
0.2475
0.4012
0.4315
K121013
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
0.37255
0.31045
0.2813
0.28895
0.3264
0.26615
0.30695
0.33255
20min
0.34985
0.33595
0.32015
0.2737
0.36105
0.2801
0.4702
0.32535
30min
0.33315
0.29125
0.33205
0.3607
0.35495
0.3605
0.3421
0.47055
40min
0.44435
0.46535
0.4083
0.4768
0.45095
0.31625
0.46675
0.4186
50min
0.31155
0.46795
0.36245
0.46145
0.34
0.35065
0.43265
0.39215
60min
0.4679
0.4767
0.4592
0.4591
0.4461
0.3544
0.5154
0.4195
70min
0.44295
0.44085
0.4873
0.4545
0.4174
0.44235
0.40865
0.42905
80min
0.43305
0.4443
0.43595
0.41495
0.49345
0.2477
0.4009
0.42965
n=2
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.3239
0.2824
0.327
0.2998
0.3292
0.3009
0.3064
0.3619
0.3238
0.286
0.3148
0.2993
0.3203
0.3057
0.3064
0.3672
0.3205
0.2938
0.3125
0.2976
0.327
0.3102
0.3149
0.3667
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.4635
0.4575
0.4364
0.4051
1.239
0.4395
0.38
0.4392
0.4384
0.4318
0.4003
0.376
1.224
0.4249
0.3504
0.4516
0.4188
0.4213
0.3989
0.3728
1.232
0.4128
0.3738
0.4561
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.3459
0.3534
0.4406
0.333
0.4258
0.3852
0.3641
0.4019
0.3528
0.3454
0.4316
0.3403
0.4087
0.372
0.368
0.4156
0.3463
0.3463
0.4161
0.3421
0.4005
0.3655
0.3595
0.405
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.399
0.4612
0.4131
0.5411
0.4163
0.3952
0.5098
0.3716
0.3697
0.4614
0.3964
0.5241
0.4031
0.3768
0.5063
0.3774
0.3678
0.4496
0.3972
0.543
0.3957
0.3772
0.4795
0.379
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.5001
0.4291
0.3549
0.4407
0.5656
0.4618
0.4496
0.4137
0.4694
0.4071
0.3409
0.4045
0.5643
0.4214
0.4179
0.4079
0.4342
0.3899
0.3389
0.3838
0.5748
0.4058
0.4087
0.4129
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.4046
0.3984
0.3951
0.3651
0.4416
0.4142
0.4031
0.4112
0.3884
0.4048
0.386
0.3649
0.4149
0.4063
0.4132
0.4091
0.4026
0.4042
0.3889
0.3695
0.4045
0.4136
0.4173
0.4046
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.4262
0.5995
0.5567
0.4127
0.4536
0.4496
0.434
0.3871
0.426
0.5448
0.5036
0.4212
0.4537
0.4617
0.4287
0.3929
0.4358
0.5057
0.4995
0.4305
0.465
0.4663
0.4464
0.404
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.4573
0.4453
0.4563
0.4572
0.4639
0.4662
0.4501
0.4169
0.4743
0.4582
0.4677
0.4712
0.4772
0.4836
0.4651
0.43
0.474
0.4602
0.47
0.4847
0.4807
0.4897
0.4682
0.4156
90min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.4692
0.4575
0.4907
0.4748
0.4833
0.4442
0.5329
0.4461
0.4818
0.4638
0.4496
0.4811
0.4931
0.4431
0.5426
0.4504
0.491
0.4667
0.5077
0.4855
0.5063
0.452
0.5399
0.4476
K121013
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
0.322733333
0.2874
0.3181
0.2989
0.3255
0.3056
0.309233333
0.365266667
20min
0.440233333
0.436866667
0.411866667
0.384633333
1.231666667
0.425733333
0.368066667
0.448966667
30min
0.348333333
0.348366667
0.429433333
0.338466667
0.411666667
0.374233333
0.363866667
0.4075
40min
0.4679
0.4087
0.3449
0.409666667
0.568233333
0.429666667
0.4254
0.4115
50min
0.398533333
0.402466667
0.39
0.3665
0.420333333
0.411366667
0.4112
0.4083
60min
0.429333333
0.55
0.519933333
0.421466667
0.457433333
0.4592
0.436366667
0.394666667
70min
0.468533333
0.454566667
0.464666667
0.471033333
0.473933333
0.479833333
0.461133333
0.420833333
80min
0.480666667
0.462666667
0.482666667
0.480466667
0.494233333
0.446433333
0.538466667
0.448033333
n=3
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.6736
0.6615
0.6838
0.6631
0.7225
0.6456
0.6674
0.6824
0.6747
0.6666
0.6785
0.6668
0.7233
0.6551
0.6593
0.6977
0.6793
0.6617
0.6822
0.6606
0.7311
0.6608
0.6474
0.6928
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.7146
0.7038
0.7116
0.705
0.6954
0.6961
0.7676
1.056
0.7152
0.6896
0.6982
0.7056
0.694
0.6954
0.7532
1.038
0.7137
0.7081
0.7047
0.6991
0.7091
0.7012
0.7359
1.043
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.8089
0.7698
0.6644
0.7044
0.7587
0.6859
0.8438
0.7259
0.7919
0.7596
0.6587
0.7152
0.7587
0.6923
0.8064
0.7172
0.8109
0.7746
0.6814
0.7109
0.7582
0.6993
0.8163
0.7161
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.7681
0.7285
0.7127
0.704
0.7995
0.7897
0.776
0.7421
0.7659
0.7262
0.7025
0.7097
0.7964
0.7878
0.7685
0.7498
0.7721
0.7307
0.7068
0.7168
0.8021
0.7851
0.7728
0.7586
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.7219
0.7292
0.7554
0.7837
0.814
0.8418
0.8384
0.9178
0.7332
0.7323
0.7592
0.7857
0.82
0.8476
0.8431
0.9303
0.7401
0.7348
0.7666
0.7929
0.8249
0.8458
0.838
0.9356
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.9014
0.8845
0.9814
0.8661
0.8337
0.8881
0.844
0.9678
0.908
0.8968
0.9881
0.8733
0.8189
0.8946
0.8348
0.9633
0.9144
0.8982
0.9851
0.8866
0.8431
0.8809
0.8333
0.956
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.942
0.9845
0.9667
0.9166
0.9135
0.9099
0.9704
0.9324
0.9411
0.9721
0.955
0.8998
0.9107
0.8965
0.9403
0.931
0.9422
0.9663
0.9567
0.8997
0.9032
0.8789
0.9528
0.9409
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
1.038
1.013
0.9944
1.024
1.025
1.012
0.9951
1.167
1.018
1.01
0.9868
1.008
0.9691
0.9953
0.9738
1.14
1.034
1.024
1.011
1.001
0.9761
0.999
0.9794
1.144
90min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.9791
1.011
1.053
1.023
1.037
0.9886
0.9325
1.116
0.9971
1.006
1.063
1.013
0.9948
1.007
0.9513
1.108
0.9818
1.011
1.071
1.038
1.023
1.002
0.9492
1.098
K121013
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
0.675866667
0.663266667
0.6815
0.6635
0.725633333
0.653833333
0.658033333
0.690966667
20min
0.7145
0.7005
0.704833333
0.703233333
0.6995
0.697566667
0.752233333
1.045666667
30min
0.8039
0.768
0.668166667
0.710166667
0.758533333
0.6925
0.822166667
0.719733333
40min
0.7687
0.728466667
0.707333333
0.710166667
0.799333333
0.787533333
0.772433333
0.750166667
50min
0.731733333
0.7321
0.7604
0.787433333
0.819633333
0.845066667
0.839833333
0.9279
60min
0.941766667
0.9743
0.959466667
0.905366667
0.909133333
0.8951
0.9545
0.934766667
70min
1.03
1.015666667
0.9974
1.011
0.990066667
1.0021
0.982766667
1.150333333
80min
0.986
1.009333333
1.062333333
1.024666667
1.018266667
0.9992
0.944333333
1.107333333
90min
0.986
1.009333333
1.062333333
1.024666667
1.018266667
0.9992
0.944333333
1.107333333
ahpC
n=1
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
6.362
6.549
6.639
5.949
7.186
9.847
10.41
12.03
6.476
6.705
6.733
6.106
7.341
10.22
10.99
12.44
6.523
6.723
6.779
6.152
7.458
10.4
11.34
12.78
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
7.824
6.91
7.313
6.918
7.257
11.4
12.64
13.56
7.894
7.043
7.469
6.967
7.373
11.4
12.94
13.86
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
8.171
8.195
8.624
8.713
8.967
11.28
13.64
16.13
8.32
8.302
8.791
8.83
8.983
11.39
13.94
16.11
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
8.366
8.753
9.2
8.517
8.855
11.49
14.86
15.97
8.386
8.733
9.19
8.55
9.053
11.87
15.28
16.2
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10.32
10.47
11.49
11.09
11.93
11.11
16.64
19.77
10.54
10.79
11.81
11.46
12.32
11.74
17.19
20.18
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
11.86
13.29
13.03
13.3
13.81
16.01
19.44
22.54
12.08
13.65
13.3
13.45
14.04
16.26
19.82
23.05
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
16.7
16.02
15.77
15.73
18.03
21.17
24.22
24.95
17.16
16.61
16.37
16.04
18.46
21.51
24.87
25.23
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
21.18
27.04
22.18
23.44
23.54
25.6
28.86
31.32
22
27.74
22.82
24.1
24.06
25.71
29.14
31.64
ahpC
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
6.453666667
6.659
6.717
6.069
7.328333333
10.15566667
10.91333333
12.41666667
20min
7.859
6.9765
7.391
6.9425
7.315
11.4
12.79
13.71
30min
8.2455
8.2485
8.7075
8.7715
8.975
11.335
13.79
16.12
40min
8.376
8.743
9.195
8.5335
8.954
11.68
15.07
16.085
50min
10.43
10.63
11.65
11.275
12.125
11.425
16.915
19.975
60min
11.97
13.47
13.165
13.375
13.925
16.135
19.63
22.795
70min
16.93
16.315
16.07
15.885
18.245
21.34
24.545
25.09
80min
16.93
16.315
16.07
15.885
18.245
21.34
24.545
25.09
sufA
tetR