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Team:Freiburg Bioware/Project/Project Description
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The Experimental System
Therapy using viral vectors is an promising approach for.... In an first step the plasmids of the AAV-2 Helper-free System were genetically modifyed by converting it into BioBricks and inserting of targeting molecules into the constructs. These plasmids were then used to transfect the producer cell line AAV-293. After an incubation of three days the viral vectors were harvested and used to transduce different target cells. The succesful transduction can then for example be measured by detecting the fluorescence of fluorescent proteins in the target cells.
Modularization of the rAAV genome
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Modulatization of the RepCap plasmid
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Modulatization of the Vectorplasmid
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Modification of the viral surface in one cloning step - The ViralBrick standard
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Midifications of the Virus Shell via Loop Insertions
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Specific biotinylation of the Viral Schell - The Biotinylation Acceptor Peptide (BAP)
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Purification of Therapeutic Viral Vectors - The His-Affinity Tag
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Targeting of integrin overexpressing cells - The RGD Motif
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Arming the Viral Vector with therapeutic antibodies - The Z34C Motif
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Testing the limit for loop insertion - The Beta-Lactamase
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Method Development
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Arming: Killing the Tumor
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All-In-One: Testing multiple modified Viral Vectors
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Virus Construction Kit - The Manual
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