Team:Freiburg Bioware/BioBricks/capsid coding

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Capsid Coding

<parttable>viral_vectors_aav_capsid</parttable>

Basic Capsidcoding Constructs



Hanna fragen...



ViralBricks for Loop replacement

ViralBricks are short nucleotide sequences derived from the gene of the viral coat proteins VP1-3 where they encode the two major surface exposed loops around the positions 453 and 587. Functional peptides are integrated in the loop tip which will be present on the surface of viral vectros that have been modified with the corresponding ViralBrick.

Different ViralBrick versions:

  • 453 integration site
  • 587 integration site
  • 587 integration site with HSPG knock-out

    • Different functional motifs:

  • Biotinylation Acceptor Peptide (BAP)
  • His-Affinity tag (His)
  • RGD-Motif for Integrin targeting
  • Z34C a antibody binding motif from proein A



    • Versions of the Rep-Vp123_p5-TATA-less



    Versions of the pCMV_Vp123

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