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Team:KIT-Kyoto/Protocol/English
From 2010.igem.org
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== Plasmid Mini Prep == | == Plasmid Mini Prep == | ||
| - | + | :*Buffer P1 (Suspension Buffer) | |
::50mM Tris-HCl and 10mM EDTA, pH8.0(25℃)50μg/ml | ::50mM Tris-HCl and 10mM EDTA, pH8.0(25℃)50μg/ml | ||
| - | + | :*Buffer P2 (Lysis Buffer) | |
::0.2M NaOH and 1%SDS | ::0.2M NaOH and 1%SDS | ||
| - | + | :*Buffer N3 (Neutralization and Binding Buffer) | |
::4M guanidine hydrochloride and 0.5M potassium acetate, pH4.2 | ::4M guanidine hydrochloride and 0.5M potassium acetate, pH4.2 | ||
| - | + | :*Buffer PB (Wash Buffer) | |
::5M guanidine hydrochloride,20mM Tris-HCl, pH6.6 (25℃) [final concentrations after addition of ethanol]with 38% ethanol. | ::5M guanidine hydrochloride,20mM Tris-HCl, pH6.6 (25℃) [final concentrations after addition of ethanol]with 38% ethanol. | ||
| - | + | :*Buffer PE (Wash Buffer) | |
::20mM NaCl, 2mM Tris-HCl, pH7.5 (25℃) [final concentrations after addition of ethanol:widh 80% ethanol | ::20mM NaCl, 2mM Tris-HCl, pH7.5 (25℃) [final concentrations after addition of ethanol:widh 80% ethanol | ||
| - | + | :*Buffer EB(Elution Buffer) | |
:10mM Tris-HCl, pH8.5(25℃) | :10mM Tris-HCl, pH8.5(25℃) | ||
Revision as of 07:31, 28 September 2010
 
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| Plasmid Mini Prep
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