Team:KIT-Kyoto/Protocol/English

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Plasmid Mini Prep

    • Buffer P1 (Suspension Buffer)
50mM Tris-HCl and 10mM EDTA, pH8.0(25℃)50μg/ml
    • Buffer P2 (Lysis Buffer)
0.2M NaOH and 1%SDS
    • Buffer N3 (Neutralization and Binding Buffer)
4M guanidine hydrochloride and 0.5M potassium acetate, pH4.2
    • Buffer PB (Wash Buffer)
5M guanidine hydrochloride,20mM Tris-HCl, pH6.6 (25℃) [final concentrations after addition of ethanol]with 38% ethanol.
    • Buffer PE (Wash Buffer)
20mM NaCl, 2mM Tris-HCl, pH7.5 (25℃) [final concentrations after addition of ethanol:widh 80% ethanol
    • Buffer EB(Elution Buffer)
10mM Tris-HCl, pH8.5(25℃)



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