INSA-Lyon/12 July 2010
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<li>ligation + transformation: (during 3 hours at room temperature)<br> | <li>ligation + transformation: (during 3 hours at room temperature)<br> | ||
<ol id="list_extra2"> | <ol id="list_extra2"> | ||
- | <li> | + | <li>BBa_J23119+BBa_K115017 in plasmid pSB1C3 (3A method): constitutive promoter + ARNth |
- | <li>BBa_E0040+BBa_B0015 | + | <li>BBa_E0040+BBa_B0015: GFP + terminator |
- | <li>BBa_J23119+BBa_K115017 | + | <li>BBa_J23119+BBa_K115017 : constitutive promoter + ARNth |
</ol> | </ol> | ||
</ul> | </ul> |
Revision as of 13:04, 30 August 2010
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July 12
- Transformation of BBa_I0500,BBa_R0065, BBa_C0062,BBa_C0161
- Extraction of BBa_B0015,BBa_J23119,BBa_E0040,BBa_C0056,BBa_K115017,BBa_125500 and gel electrophoresis
- Digestion:
part enzyme 1 enzyme 2 BBa_B0015 E X BBa_K115017 E X BBa_K115017 X P BBa_J23119 E S BBa_E0040 E S -
no gel picture available
- ligation + transformation: (during 3 hours at room temperature)
- BBa_J23119+BBa_K115017 in plasmid pSB1C3 (3A method): constitutive promoter + ARNth
- BBa_E0040+BBa_B0015: GFP + terminator
- BBa_J23119+BBa_K115017 : constitutive promoter + ARNth