INSA-Lyon/12 July 2010
From 2010.igem.org
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<ul> | <ul> | ||
- | <li>Transformation of | + | <li>Transformation of BBa_I0500,BBa_R0065, BBa_C0062,BBa_C0161 <br> |
- | <li>Extraction of | + | <li>Extraction of BBa_B0015,BBa_J23119,BBa_E0040,BBa_C0056,BBa_K115017,BBa_125500 and gel electrophoresis <br><br> |
<p> | <p> | ||
<img class="image" src="" alt="rajouter la photo de gel" /> | <img class="image" src="" alt="rajouter la photo de gel" /> | ||
Line 47: | Line 47: | ||
<tr> | <tr> | ||
- | <td> | + | <td>BBa_B0015</td> |
<td>E</td> | <td>E</td> | ||
<td>X</td> | <td>X</td> | ||
Line 53: | Line 53: | ||
<tr> | <tr> | ||
- | <td> | + | <td>BBa_K115017</td> |
<td>E</td> | <td>E</td> | ||
<td>X</td> | <td>X</td> | ||
Line 59: | Line 59: | ||
<tr> | <tr> | ||
- | <td> | + | <td>BBa_K115017</td> |
<td>X</td> | <td>X</td> | ||
<td>P</td> | <td>P</td> | ||
Line 65: | Line 65: | ||
<tr> | <tr> | ||
- | <td> | + | <td>BBa_J23119</td> |
<td>E </td> | <td>E </td> | ||
<td>S</td> | <td>S</td> | ||
Line 71: | Line 71: | ||
<tr> | <tr> | ||
- | <td> | + | <td>BBa_E0040</td> |
<td>E</td> | <td>E</td> | ||
<td>S</td> | <td>S</td> | ||
Line 83: | Line 83: | ||
<li>ligation + transformation: (during 3 hours at room temperature)<br> | <li>ligation + transformation: (during 3 hours at room temperature)<br> | ||
<ol id="list_extra2"> | <ol id="list_extra2"> | ||
- | <li>3A method: | + | <li>3A method:BBa_E0040+BBa_J23119 in plasmid pSB1C3 |
- | <li> | + | <li>BBa_E0040+BBa_B0015 |
- | <li> | + | <li>BBa_J23119+BBa_K115017 |
</ol> | </ol> | ||
</ul> | </ul> |
Revision as of 12:16, 30 August 2010
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July 12
- Transformation of BBa_I0500,BBa_R0065, BBa_C0062,BBa_C0161
- Extraction of BBa_B0015,BBa_J23119,BBa_E0040,BBa_C0056,BBa_K115017,BBa_125500 and gel electrophoresis
- Digestion:
part enzyme 1 enzyme 2 BBa_B0015 E X BBa_K115017 E X BBa_K115017 X P BBa_J23119 E S BBa_E0040 E S -
no gel picture available
- ligation + transformation: (during 3 hours at room temperature)
- 3A method:BBa_E0040+BBa_J23119 in plasmid pSB1C3
- BBa_E0040+BBa_B0015
- BBa_J23119+BBa_K115017