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Team:Newcastle/18 August 2010
From 2010.igem.org
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!''yneA'' 2 | !''yneA'' 2 | ||
!''yneA'' 3 | !''yneA'' 3 | ||
| - | ! | + | !pSB1C3 |
| + | !pGFPrrnB | ||
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!Concentration of DNA ng/µl | !Concentration of DNA ng/µl | ||
| - | ! | + | !3.4 |
| - | ! | + | !4.7 |
| - | ! | + | !5.4 |
| - | ! | + | !2.2 |
| + | !18.5 | ||
|} | |} | ||
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===Gel=== | ===Gel=== | ||
Revision as of 15:53, 19 August 2010
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Contents |
yneA
Aims
Today we aim to extract yneA in the biobrick comapatible vector from yesterday's overnight culture. We aim to check the concentration from the miniprep with the nanodrop then digest the plasmid with EcoR1 and Nhe1, ligate into GFPrrnb and transform cells to grow on a plate overnight.
Materials and Protocol
Please refer to protocols mentioned below for materials required:
- Plasmid extraction protocol
- Nanodrop protocol
- Restriction digest protocol
- Gel electrophoresis protocol
- Gel extraction protocol
Results
Nanodrop results
| yneA 1 | yneA 2 | yneA 3 | pSB1C3 | pGFPrrnB | |
|---|---|---|---|---|---|
| Concentration of DNA ng/µl | 3.4 | 4.7 | 5.4 | 2.2 | 18.5 |
Gel
Gel extraction of pSB1C3 plasmid
Aim
The aim of this experiment is to extract EcoR1 digested pSB1C3 plasmid that have been extracted on 3 August 2010. The digested plasmid is then used for PCR with a new set of primers that have been designed to amplify the pSB1C3 sequence.
Materials and Protocol
Please refer to: Gel electrophoresis and Gel extraction.
Results
   
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