Team:Caltech/Week 6

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Revision as of 18:42, 21 July 2010

iGEM 2010

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Group meeting!
- Discussed issues with cloning and next week's focus.
Performed a digest (now for 2hr) and ligations (L18-23). Transformed into DH5α.
Turns out that the sequencing primers we ordered (G00100 & G00101) are incorrect - they appear to bind on the wrong sides of the insert. We ordered new primers designed to bind to the BioBrick prefix and suffix.
Ran gel of digest product to verify correct bands.

Digest test gel with US and DS digested bricks. (7/19)

All ligation transformations failed. Will try 16hr ligations and purifying digest products with a PCR purification kit.
Made new growth curves testing AIBN effect on cells in LB
Performed another live/dead assay
- Had solutions of cells in glycerol and AIBN
- Plated cells every hour for three hours
- Combined glycerol solutions with LB and plated the solution

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 ** Discussed issues with cloning and next week's focus.
 * Performed a digest (now for 2hr) and ligations (L18-23). Transformed into DH5&alpha;.
+* Turns out that the sequencing primers we ordered (G00100 & G00101) are incorrect - they appear to bind on the wrong sides of the insert. We ordered new primers designed to bind to the BioBrick prefix and suffix.
 * Ran gel of digest product to verify correct bands.
 [[Image:DigestsGel7-19.jpg|200px|thumb|right|Digest test gel with US and DS digested bricks. (7/19)]]