Team:Freiburg Bioware/Project/Results
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Revision as of 03:14, 28 October 2010
Results
Highlights
Modularization
Gene of interest |
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TargetingOne aim of our research is on the one hand to knock down the natural tropism of the Adeno-associates virus particles and on the other hand to specifically target tumor cells. This is achieved by genetic engineering of the virus surface. For this purpose, two different strategies were developed, including Targeting via Loops or fusion to the N-terminus of the viral protein VP2. |
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ArmingThe specifically targeted tumor cells were killed by prodrug activation approaches. Viral particles were charged with thymidine kinase and cytosine deaminase constructs to induce apoptosis in cancer cells upon delivery of ganciclovir or 5-Fluorocytosine, respectively. |
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