BIOTEC Dresden/Notepad/16 August 2010
From 2010.igem.org
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A restriction digest on the purified plasmids was carried out to confirm if the ligation worked and the insert has the correct size. | A restriction digest on the purified plasmids was carried out to confirm if the ligation worked and the insert has the correct size. | ||
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{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} |
Latest revision as of 21:58, 27 October 2010
AHL assay
An AHL assay with part 24 (BBA_T9002) was carried in a 96 well plate with a step of 150 nM AHL concentration (range 150 - 1500 nM). Lb was used for blank, in addition pure bacterial culture (OD=6) was used as control.
Reading done at an emission wavelength of 535 nm and excitation wavelength 485 nm
However, a different amount of bacterial culture was used for wells of different AHL concentration, a necessary improvement for the next detection will be to introduce an equal nr of cells in each well and adjust the volume difference with LB
Fusion Protein
A restriction digest on the purified plasmids was carried out to confirm if the ligation worked and the insert has the correct size.
July |
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August |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
September |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | |
October |
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