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BIOTEC Dresden/Notepad/16 August 2010
From 2010.igem.org
AHL assay
An AHL assay with part 24 (BBA_T9002) was carried in a 96 well plate with a step of 150 nM AHL concentration (range 150 - 1500 nM). Lb was used for blank, in addition pure bacterial culture (OD=6) was used as control.
Reading done at an emission wavelength of 535 nm and excitation wavelength 485 nm
However, a different amount of bacterial culture was used for wells of different AHL concentration, a necessary improvement for the next detection will be to introduce an equal nr of cells in each well and adjust the volume difference with LB
Fusion Protein
A restriction digest on the purified plasmids was carried out to confirm if the ligation worked and the insert has the correct size.
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