File list

Contents 1 Modeling 2 Modularaization of GOI - Results 3 Introduction to Adeno-Associated Virus Serotype 2 4 Trafficking 5 Helper genes 6 Gene therapy 7 Cytosine deaminase 8 Loop insertions 9 Tagging 10 Targeting 11 N-terminal fusion 12 VP1 insertion 13 Targeting molecules

Modeling

Media:Freiburg10_VirusProduction01.png

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Media:Freiburg10_VirusInfection01.png

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Media:Freiburg10_VirusInfection03.png

Media:Freiburg10 VirusInfectionScheme01.png

Media:Freiburg10 VirusProductionScheme01.png

Media:Freiburg10_VirusProductionData01.png

Media:Freiburg10 VirusInfectionScheme01.png

Media:Freiburg10_VirusProductionTimeLapsemVenus.gif

Media:Freiburg10_VirusProductionTimeLapsemVenus.png

Media:Freiburg10_VirusProductionTimeLapsemCherry.gif

Media:Freiburg10_VirusProductionTimeLapsemCherry.png

Modularaization of GOI - Results

Media:Freiburg10_Overview_BBa_Vectorplasmid.png

Media:Freiburg10_Overview_Assembly_GOI.png

Media:Freiburg10_TheoreticalCloning_ITR_CMV_beta.png

Media:Freiburg10_Cloning_Intermediate_GOI.png

Media:Freiburg10_Cloning_Full_GOI.png

Media:Freiburg10_VirusInfection03.png

Media:Freiburg10 VirusInfectionScheme01.png

Media:Freiburg10 VirusProductionScheme01.png

Media:Freiburg10_VirusProductionData01.png

Media:Freiburg10 VirusInfectionScheme01.png

Media:Freiburg10_VirusProduction01.png

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Media:Freiburg10_VirusInfection01.png

Media:Freiburg10_FACS_woHGH.png

Media:Freiburg10_FACS_withHGH.png

Media:Freiburg10_Diagram_hGH.png

Media:Freiburg10_FACS_betaglobin.png

Media:Freiburg10_FACS_withbetaglobin.png

Media:Freiburg10 VirusInfectionScheme01.png

Media:Freiburg10_Diagram_betaglobin.png.png

Media:Freiburg10_FACS_FULL_pSB1c3_mVenus.png

Media:Freiburg10_FACS_FULL_pAAV_mVenus.png

Media:Freiburg10_Diagram_FULL_GOI.png

Introduction to Adeno-Associated Virus Serotype 2

Media:Freiburg10_genomic_organisation_wt.png

Media:Freiburg10_organization_ITRs.png

Media:Freiburg10_Overview_AAV-2_replication.png

Media:Freiburg10_regulation_viral_promotors.png

Media:Freiburg10_p5_promoter_wtAAV2.png

Media:Freiburg10_p5_TATA_less.png

Media:Freiburg10_DNA_Loop_P5_to_sp1.png

Media:Freiburg10_Rep_proteins_organization.png

Media:Freiburg10_Crystal_structure_SF3helicase.png

Trafficking

Media:Freiburg10_viral_trafficking.png

Media:Freiburg10_cellular_surface_HSPG.png

Media:Freiburg10_Clathrin_triskelions.png

Helper genes

Media:Freiburg10_Helper_free_system.png

Media:Freiburg10_pHelper.png

Gene therapy

Media:Freiburg10_overview_GDEPT.png

Media:Freiburg10_Bystander_effect.png

Cytosine deaminase

Media:Freiburg10_Flourocytosine_pathway.png

Loop insertions

Media:Freiburg10_3D_AC_of_453_587_loops.png

Media:Freiburg10_cloning_viral_bricks.png

Tagging

Media:Freiburg10_BAP.png

Media:Freiburg10_His.png

Targeting

Media:Freiburg10_RGD_motif.png

Media:Freiburg10_Z34C_motif.png

Media:Freiburg10_Z34C_Z38_ZDomain_helix.png

N-terminal fusion

Media:Freiburg10_N_terminal_fusion_approach.png

Media:Freiburg10_Cotransfected_plasmids.png

VP1 insertion

Media:Freiburg10_pSB1C3_VP1up_NLS_Fig27.png

Media:Freiburg10_VP1_insertion.png

Targeting molecules

Media:Freiburg10_Nucleotide_sequence_ZEGFR.png

Media:Freiburg10_Darpin.png

Media:Freiburg10 VirusInfectionScheme01.png

Media:Freiburg10_Diagram_betaglobin.png.png

Media:Freiburg10_FACS_FULL_pSB1c3_mVenus.png

Media:Freiburg10_FACS_FULL_pAAV_mVenus.png

Media:Freiburg10_Diagram_FULL_GOI.png