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BIOTEC Dresden/Notepad/1 September 2010
From 2010.igem.org
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The ligation mix was then transformed by electroporation and plated and kept for overnight incubation. | The ligation mix was then transformed by electroporation and plated and kept for overnight incubation. | ||
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'''Fusion Protein''' | '''Fusion Protein''' | ||
A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion. | A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion. | ||
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{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Revision as of 14:04, 24 October 2010
Ligation of following parts with 6e (RBS)
4a, 14f, 20f, 21f
The ligation mix was then transformed by electroporation and plated and kept for overnight incubation.
Fusion Protein
A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion.
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