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BIOTEC Dresden/Notepad/1 September 2010
From 2010.igem.org
Ligation of following parts with 6e (RBS)
4a, 14f, 20f, 21f
The ligation mix was then transformed by electroporation and plated and kept for overnight incubation.
Fusion Protein
A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion.
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