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Team:Freiburg Bioware/BioBricks/capsid coding
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<br><br><!---Versions of the pCMV_Vp123---> | <br><br><!---Versions of the pCMV_Vp123---> | ||
<center><h1>Versions of the pCMV_Vp123 </h1></center> | <center><h1>Versions of the pCMV_Vp123 </h1></center> | ||
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The different versions of pCMV_VP123 are ment for the production of mosaic viruses by transfecting this plamid together with [AAV2]-Rep-VP123_P5-TATA-less. The composite part pCMV_VP123 contains the ORF of the Viral Coat Proteins VP123 under the control of the control of the Cytomegalovirus promoter. By providing modified and unmodified viral coat proteins the resulting viral vector will be a chimera. The ratio of modified to unmodified viral coat proteins will depend on the number of plasmids a cell was transfected with and has impect on the functionality of the viral vector.</div> | The different versions of pCMV_VP123 are ment for the production of mosaic viruses by transfecting this plamid together with [AAV2]-Rep-VP123_P5-TATA-less. The composite part pCMV_VP123 contains the ORF of the Viral Coat Proteins VP123 under the control of the control of the Cytomegalovirus promoter. By providing modified and unmodified viral coat proteins the resulting viral vector will be a chimera. The ratio of modified to unmodified viral coat proteins will depend on the number of plasmids a cell was transfected with and has impect on the functionality of the viral vector.</div> | ||
Revision as of 10:45, 24 October 2010
Capsid Coding
Basic Capsidcoding Constructs
Hanna fragen...
ViralBricks for Loop replacement
ViralBricks are short nucleotide sequences derived from the gene of the viral coat proteins VP1-3 where they encode the two major surface exposed loops around the positions 453 and 587. Functional peptides are integrated in the loop tip which will be present on the surface of viral vectros that have been modified with the corresponding ViralBrick.
Different ViralBrick versions:
Different functional motifs:
Versions of the Rep-Vp123_p5-TATA-less
Versions of the pCMV_Vp123
The different versions of pCMV_VP123 are ment for the production of mosaic viruses by transfecting this plamid together with [AAV2]-Rep-VP123_P5-TATA-less. The composite part pCMV_VP123 contains the ORF of the Viral Coat Proteins VP123 under the control of the control of the Cytomegalovirus promoter. By providing modified and unmodified viral coat proteins the resulting viral vector will be a chimera. The ratio of modified to unmodified viral coat proteins will depend on the number of plasmids a cell was transfected with and has impect on the functionality of the viral vector.
