Team:KIT-Kyoto/Protocol

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About protocol

As it is of common knowledge, iGEM is carried out within the framework of a protocol common to all teams. Whereas most of the teams are registered on this page, we have uploaded not only the English protocol but also the Japanese one, e.g., our mother tongue version. This is the common protocol with some improvements after we translated it into Japanese.Publishing the Japanese protocol in will be of help to other new Japanese teams in the future. Furthermore, this will be linked to an improvement of iGEM’s name identification in Japan and will enhance the public recognition of synthetic biology. It will also promote Science and Communication among people within not only the scientific but with the non-scientific communities as well. We believe that these improvements will constitute a contribution to the illustration of the public in general.

Contents

Japanese トランスフォーメーション アルカリミニプレップ ポリメラーゼ連鎖反応(PCR) 制限酵素処理 アガロースゲル電気泳動 ゲルからのDNA抽出 ライゲーション コンピテント細胞の作製 シークエンス グリセロールストック LB培地 2xYT培地 SOB培地 SOC培地

English Bacterial transformation DNA miniprep Polymerase Chain Reaction(PCR) DNA digestion by restriction enzymes Agarose gel electrophoresis Isolation of DNA fragments from agarose gel DNA ligation Preparing chemically competent cells Sequencing Glycerol stock LB culture medium 2xYT culture medium SOB culture medium SOC culture medium

Protocol

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