Team:Freiburg Bioware/NoteBook/Labjournal/March

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1. Labday 31.03.2010

Sequence analysis

Investigators: Christian W., Bea, Kira, Achim, Kerstin, Hanna, Christian L. (Tobias, Kristian)

Theoretical cloning

Sequence analysis (Alignement) of Stratagene Vector with

AAV2
AAV5
AAV7
AAV8

result: Media:AAV_alignment.pdf
Notably, the alignment showed differences in the various AAV2 sequences, some of which are leading to frameshifts. The sequences date from 1983 and might not be completely accurate. We therefore rely on the Stratagene sequence from now on.

To do:

download and compare further AAV serotypes. (In Geneious)
compare literature
find out possible ways to modify the Capsid

=> Go to Labjournal April (labday 2 - 5)

@@ Line 38: / Line 38: @@
-Sequence analysis (Alignement) of the Stratagene Vector with
+Sequence analysis (Alignement) of Stratagene Vector with
 *AAV2
 *AAV5
@@ Line 47: / Line 47: @@
 [[Media:AAV_alignment.pdf]]
 <br>
-Seltsamerweise waren vor allem bei AAV2 einige auffällige Sequenzunterschiede vorhanden, die zu Frameshifts führten. Der Grund hierfür könnte sein, dass die Sequenzdaten von 1983 stammten und deshalb nicht als vollständig richtig eingestuft werden können. Im Zweifelsfall ist es besser sich auf die Sequenz von Stratagene zu verlassen.
 Notably, the alignment showed differences in the various AAV2 sequences, some of which are leading to frameshifts. The sequences date from 1983 and might not be completely accurate. We therefore rely on the Stratagene sequence from now on.