Team:KIT-Kyoto/Protocol/English

From 2010.igem.org

(Difference between revisions)
 
Line 11: Line 11:
</head></html>
</head></html>
{{Template:KIT-Kyoto/menu}}
{{Template:KIT-Kyoto/menu}}
-
 
+
<table border="0" width="965px" align="center"><tr><td>
-
<table border=0 width="965px" align="center"><tr><td width="165px" valign="top" align="left"><div>
+
[[Team:KIT-Kyoto|Home]] > [[Team:KIT-Kyoto/Note|Notebook]] > [[Team:KIT-Kyoto/Protocol|Protocol]] > [[Team:KIT-Kyoto/Protocol/English|English]]</td></tr></table>
-
{{Template:KIT-Kyoto/menu10}}
+
<table border=0 width="965px" align="center"><tr><td width="165px" valign="top" align="left"><div>{{Template:KIT-Kyoto/menu10}}
-
</div></td><td width="800px" valign="top" align="left"><div style="padding:3px;">
+
</div></td><td width="800px" valign="top" align="left"><div id="MIGI">
== Plasmid Mini Prep ==
== Plasmid Mini Prep ==

Latest revision as of 08:58, 9 October 2010



Home > Notebook > Protocol > English

Plasmid Mini Prep

  • Buffer P1 (Suspension Buffer)
50mM Tris-HCl and 10mM EDTA, pH8.0(25℃)50μg/ml
  • Buffer P2 (Lysis Buffer)
0.2M NaOH and 1%SDS
  • Buffer N3 (Neutralization and Binding Buffer)
4M guanidine hydrochloride and 0.5M potassium acetate, pH4.2
  • Buffer PB (Wash Buffer)
5M guanidine hydrochloride,20mM Tris-HCl, pH6.6 (25℃) [final concentrations after addition of ethanol]with 38% ethanol.
  • Buffer PE (Wash Buffer)
20mM NaCl, 2mM Tris-HCl, pH7.5 (25℃) [final concentrations after addition of ethanol:widh 80% ethanol
  • Buffer EB(Elution Buffer)
10mM Tris-HCl, pH8.5(25℃)



Retrieved from "http://2010.igem.org/Team:KIT-Kyoto/Protocol/English"