Team:Newcastle/18 August 2010

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(→Results)
(→Gel extraction of pSB1C3 plasmid)
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=Gel extraction of pSB1C3 plasmid=
 
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==Aim==
 
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The aim of this experiment is to extract EcoR1 digested pSB1C3 plasmid that have been extracted on [[Team:Newcastle/3_August_2010| 3 August 2010]]. The digested plasmid is then used for PCR with a new set of primers that have been designed to amplify the pSB1C3 sequence.
 
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==Materials and Protocol==
 
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Please refer to: [[Team:Newcastle/Gel electrophoresis| Gel electrophoresis]] and [[Team:Newcastle/Gel extraction| Gel extraction]].
 
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==Results==
 
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The gel extraction was successful, and the band was found at the correct location.
 
'''Go back to our main [[Team:Newcastle/notebook| Lab book]] page'''
'''Go back to our main [[Team:Newcastle/notebook| Lab book]] page'''

Revision as of 01:32, 26 October 2010

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Contents

yneA

Aims

Today we aim to extract yneA in the biobrick comapatible vector from yesterday's overnight culture. We aim to check the concentration from the miniprep with the nanodrop then digest the plasmid with EcoR1 and Nhe1, ligate into pGFPrrnB and transform cells to grow on a plate overnight.

Materials and Protocol

Please refer to protocols mentioned below for materials required:

Results

Nanodrop results

yneA 1 yneA 2 yneA 3 yneA 4
Concentration of DNA ng/µl 247.3 233.6 456.6 140.0


Nanodrop1882010.jpeg Nanodrop18820102.jpeg Nanodrop18820103.jpeg Nanodrop18820104.jpeg


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