From 2010.igem.org
(Difference between revisions)
|
|
| Line 23: |
Line 23: |
| | | | |
| | ==Thursday 7/22== | | ==Thursday 7/22== |
| - | | + | * Ran gel of digest products from yesterday to verify correctness of the digestion. |
| | + | [[Image:Digest22-07.jpg|200px|thumb|right|Digest test gel with upstream, downstream, and backbone digested bricks. (7/22)]] |
| | ==Friday 7/23== | | ==Friday 7/23== |
| | | | |
Revision as of 01:33, 23 July 2010
iGEM 2010
Home
People
Project Details
Protocols
Completed Systems
Notebook
Biosafety
Human Impact
References
Support
|
Monday 7/19
- Group meeting!
- Discussed issues with cloning and next week's focus.
- Performed a digest (now for 2hr) and ligations (L18-23). Transformed into DH5α.
- Turns out that the sequencing primers we ordered (G00100 & G00101) are incorrect - they appear to bind on the wrong sides of the insert. We ordered new primers designed to bind to the BioBrick prefix and suffix.
- Ran gel of digest product to verify correct bands.
 Digest test gel with US and DS digested bricks. (7/19) Tuesday 7/20
- All ligation transformations failed. Will try 16hr ligations and purifying digest products with a PCR purification kit.
- Made new growth curves testing AIBN effect on cells in LB
- Performed another live/dead assay
- Had solutions of cells in glycerol and AIBN
- Plated cells every hour for three hours
- Combined glycerol solutions with LB and plated the solution
Wednesday 7/21
Thursday 7/22
- Ran gel of digest products from yesterday to verify correctness of the digestion.
 Digest test gel with upstream, downstream, and backbone digested bricks. (7/22) Friday 7/23
Weekend 7/24-25
|
"
