Team:Caltech/Week 6

From 2010.igem.org

(Difference between revisions)
m
Line 8: Line 8:
* Performed a digest (now for 2hr) and ligations (L18-23). Transformed into DH5α.
* Performed a digest (now for 2hr) and ligations (L18-23). Transformed into DH5α.
* Ran gel of digest product to verify correct bands.
* Ran gel of digest product to verify correct bands.
-
[Image:DigestsGel7-19.jpg]
+
[[Image:DigestsGel7-19.jpg|200px|thumb|right|Digest test gel with US and DS digested bricks. (7/19)]]
==Tuesday 7/20==
==Tuesday 7/20==

Revision as of 18:44, 20 July 2010


iGEM 2010



Home

People

Project Details

Protocols

Completed Systems

Notebook

Biosafety

Human Impact

References

Support


Caltech logo watermark.png


Monday 7/19

  • Group meeting!
    • Discussed issues with cloning and next week's focus.
  • Performed a digest (now for 2hr) and ligations (L18-23). Transformed into DH5α.
  • Ran gel of digest product to verify correct bands.
Digest test gel with US and DS digested bricks. (7/19)

Tuesday 7/20

  • All ligation transformations failed. Will try 16hr ligations and purifying digest products with a PCR purification kit.


Wednesday 7/21

Thursday 7/22

Friday 7/23

Weekend 7/24-25

 
Error creating thumbnail: /websites/2010.igem.org/wiki/bin/ulimit4.sh: line 4: convert: command not found
Caltech footer.png


Locations of visitors to this page