Team:Caltech/Week 7
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==Monday 7/26== | ==Monday 7/26== | ||
+ | * Started PCR reaction to extract the HSP (K112400) from its Berkeley Standard backbone (BBb). (Used same PCR parameters as CPCR from 7/22. | ||
+ | * Group meeting! | ||
+ | ** By next week: Need to design test-construct experiments, as they will be ready sometime early next week. | ||
+ | *** Can use PCR machine to achieve easy temperature gradients | ||
+ | *** Use spectrophotometer and eventually microscope to assay fluorescence. | ||
+ | *** Consider a plate assay? | ||
+ | ** AIBN issues: | ||
+ | *** Consider using low-concentration DMSO to help dissolve AIBN in LB/cell mixture. | ||
+ | *** Need to figure out how much unreacted (or otherwise) AIBN remains after mixing with cells. | ||
+ | *** Need to test crosslinking using hydrolyzed and epoxidized oil mixed with lysate and with cells to tweak concentration of AIBN needed, and to see what it makes. | ||
+ | ** TGase: | ||
+ | *** purchase urea? | ||
+ | *** To prevent a solid plug forming upon addition of TGase powder to sample, prepare liquid stock of concentrated TGase. | ||
+ | ** Wiki: | ||
+ | *** Should visit biofab.org for research material for human impact segment. | ||
+ | ** In general, we should set goals for both the end of SURF and for the end of the summer and determine what we need to do to get on track for them. | ||
+ | *** Printing in 2D | ||
+ | *** Working AND gate, at least in bulk fluid | ||
+ | *** Characterization of 3D hydrogel/plastic | ||
+ | *** Crosslinking data | ||
+ | * PCR purified K112400 PCR product & digested as plasmid insert. | ||
+ | * Transformed RFP bricks of verious resistances to use as backbone for future ligations | ||
+ | * Ran 2 gels of today's CPCR products: | ||
==Tuesday 7/27== | ==Tuesday 7/27== | ||
+ | * | ||
==Wednesday 7/28== | ==Wednesday 7/28== |
Revision as of 04:56, 28 July 2010
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Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15
Monday 7/26
Tuesday 7/27Wednesday 7/28Thursday 7/29Friday 7/30Weekend 7/31-8/1
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