Team:Caltech/Week 6

From 2010.igem.org

(Difference between revisions)
Line 4: Line 4:
==Monday 7/19==
==Monday 7/19==
 +
* Group meeting!
 +
** Discussed issues with cloning and next week's focus.
 +
* Performed a digest (now for 2hr) and ligations (L18-23). Transformed into DH5α.
 +
* Ran gel of digest product to verify correct bands.
==Tuesday 7/20==
==Tuesday 7/20==
 +
* All ligation transformations failed. Will try 16hr ligations and purifying digest products with a PCR purification kit.
 +
==Wednesday 7/21==
==Wednesday 7/21==

Revision as of 18:08, 20 July 2010


iGEM 2010



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Monday 7/19

  • Group meeting!
    • Discussed issues with cloning and next week's focus.
  • Performed a digest (now for 2hr) and ligations (L18-23). Transformed into DH5α.
  • Ran gel of digest product to verify correct bands.

Tuesday 7/20

  • All ligation transformations failed. Will try 16hr ligations and purifying digest products with a PCR purification kit.


Wednesday 7/21

Thursday 7/22

Friday 7/23

Weekend 7/24-25

 
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