File list

Contents 1 Modeling 2 Time lapse 3 Structure Modeling 4 Modularaization of GOI - Results 5 Introduction to Adeno-Associated Virus Serotype 2 6 Trafficking 7 Helper genes 8 Gene therapy 9 Cytosine deaminase 10 Loop insertions 11 Tagging 12 Targeting 13 N-terminal fusion 13.1 Results 14 VP1 insertion 15 Targeting molecules 16 Favorite parts 17 HSPG KO

Modeling

Media:Freiburg10_VirusProduction01.png

Media:Freiburg10_VirusProduction02.png

Media:Freiburg10_VirusProduction03.png

Media:Freiburg10_VirusInfection01.png

Media:Freiburg10_VirusInfection02.png

Media:Freiburg10_VirusInfection03.png

Media:Freiburg10 VirusInfectionScheme01.png

Media:Freiburg10 VirusProductionScheme01.png

Media:Freiburg10_VirusProductionData01.png

Media:Freiburg10 VirusInfectionScheme01.png

Media:Freiburg10_VirusProductionTimeLapsemVenus.gif

Media:Freiburg10_VirusProductionTimeLapsemVenus.png

Media:Freiburg10_VirusProductionTimeLapsemCherry.gif

Media:Freiburg10_VirusProductionTimeLapsemCherry.png

Media:Freiburg10_VirusInfectionLogo.png

Media:Freiburg10_VirusProductionLogo.png

Media:Freiburg10_StructureModelingLogo.png

Time lapse

Media:Freiburg10_production_Venus_mCherry_Brightfield.mov

Media:Freiburg10_TimeLapse_HT10180_TK_GMK_400px.gif

Media:Freiburg10_TimeLapse_A431_TK_GMK_400px.gif

Media:Freiburg10_TimeLapse_AAV-293_mVenus+GOI_mCherry_390px.gif

Media:Freiburg10_TimeLapse_AAV-293_mVenus+GOI_mCherry_400px.gif

Structure Modeling

Media:Freiburg10_rot250trans.gif

Media:Freiburg10_AAVassem500-60.gif

Media:Freiburg10_AAVassem500-100.gif

Media:Freiburg10_AAVassem500-100.mov

Media:Freiburg10_AAV_complete.jpg

Media:Freiburg10_AAV_singleVP3.jpg

Media:Freiburg10_AAV_singleVP3surface.jpg

Media:Freiburg10_AAV_VP3star.jpg

Media:Freiburg10_AAV_slice.gif

Media:Freiburg10_AAV_sliceSur.gif

Media:Freiburg10_PleaseWait.gif

Media:Freiburg10_AAV_assembly_600px.png

Media:Freiburg10_AAV_assembly_400px.png

Media:Freiburg10_AAV_assembly_scheme_400px.png

Modularaization of GOI - Results

Media:Freiburg10_Overview_BBa_Vectorplasmid.png

Media:Freiburg10_Overview_Assembly_GOI.png

Media:Freiburg10_TheoreticalCloning_ITR_CMV_beta.png

Media:Freiburg10_Cloning_Intermediate_GOI.png

Media:Freiburg10_Cloning_Full_GOI.png

Media:Freiburg10_Microscopy_Overlay_mVenus_1_GOI.png

Media:Freiburg10_Microscopy_Overlay_grey_1_GOI.png

Media:Freiburg10_Microscopy_Overlay_mVenus_2_GOI.png

Media:Freiburg10_Microscopy_phasecontrast_2_GOI.png

Media:Freiburg10 VirusInfectionScheme01.png

Media:Freiburg10_VirusProduction01.png

Media:Freiburg10_VirusProduction02.png

Media:Freiburg10_VirusProduction03.png

Media:Freiburg10_VirusInfection01.png

Media:Freiburg10_FACS_woHGH.png

Media:Freiburg10_FACS_withHGH.png

Media:Freiburg10_Diagram_hGH.png

Media:Freiburg10_FACS_betaglobin.png

Media:Freiburg10_FACS_withbetaglobin.png

Media:Freiburg10 VirusInfectionScheme01.png

Media:Freiburg10_Diagram_betaglobin.png.png

Media:Freiburg10_FACS_FULL_pSB1c3_mVenus.png

Media:Freiburg10_FACS_FULL_pAAV_mVenus.png

Media:Freiburg10_Diagram_FULL_GOI.png

Introduction to Adeno-Associated Virus Serotype 2

Media:Freiburg10_genomic_organisation_wt.png

Media:Freiburg10_organization_ITRs.png

Media:Freiburg10_Overview_AAV-2_replication.png

Media:Freiburg10_regulation_viral_promotors.png

Media:Freiburg10_p5_promoter_wtAAV2.png

Media:Freiburg10_p5_TATA_less.png

Media:Freiburg10_DNA_Loop_P5_to_sp1.png

Media:Freiburg10_Rep_proteins_organization.png

Media:Freiburg10_Crystal_structure_SF3helicase.png

Media:Freiburg10_PLA2-mechanism.PNG

Media:Freiburg10_PLA2-recognition_site.jpg‎

Media:Freiburg10_VP1-3_overview.jpg

Media:Freiburg10_Rep40.jpg

Trafficking

Media:Freiburg10_viral_trafficking.png

Media:Freiburg10_cellular_surface_HSPG.png

Media:Freiburg10_Clathrin_triskelions.png

Helper genes

Media:Freiburg10_Helper_free_system.png

Media:Freiburg10_pHelper.png

Gene therapy

Media:Freiburg10_overview_GDEPT.png

Media:Freiburg10_Bystander_effect.png

Cytosine deaminase

Media:Freiburg10_Flourocytosine_pathway.png

Loop insertions

Media:Freiburg10_3D_AC_of_453_587_loops.png

Media:Freiburg10_cloning_viral_bricks.png

Tagging

Media:Freiburg10_BAP.png

Media:Freiburg10_His.png

Targeting

Media:Freiburg10_RGD_motif.png

Media:Freiburg10_Z34C_motif.png

Media:Freiburg10_Z34C_Z38_ZDomain_helix.png

Media:Freiburg10_HSPG.png

Media:Freiburg10_HSPG_chemical_structure.png

N-terminal fusion

Results

Media:Freiburg10 Cloning VP2Fusion2.PNG

Media:Freiburg10 Cloning VP2FusionH.PNG

Media:Freiburg10 CloningGel DARPin.png

Media:Freiburg10 CMCSchema.png

Media:Freiburg10 ColonyPCR.png

Media:Freiburg10 FACS VP1Ins A431.png

Media:Freiburg10 FACS VP1Ins HT1080.png

Media:Freiburg10 FACS VP2 A431.png

Media:Freiburg10 FACS VP2Fusion HT1080 Diagramm.png

Media:Freiburg10 Gel VP1ins.png

Media:Freiburg10 GelVP2Fusion2tog.PNG

Media:Freiburg10 GelVP2Fusion2tog2.PNG

Media:Freiburg10 LinkerSchema.png

Media:Freiburg10 NLS mVenus mCherry.png

Media:Freiburg10 NLSSchema.png

Media:Freiburg10 Overview VP1Insertion.png

Media:Freiburg10 Overview VP2Fusion.png

Media:Freiburg10 Primer ColonyPCR.png

Media:Freiburg10 qPCR VP1Insnew.PNG

Media:Freiburg10 qPCR VP2Fus.PNG

Media:Freiburg10 SchemaVP2Fusion.PNG

Media:Freiburg10 TargetingMotifSchema.png

Media:Freiburg10 test supernatant pellet.png

Media:Freiburg10 TestDARPin.png

Media:Freiburg10 TimeLapseA431.png

Media:Freiburg10 TimeLapseHT1080.png

Media:Freiburg10 VP1InsertionSchema.PNG

Media:Freiburg10 VP1KO.PNG

Media:Freiburg10 VP1upSchema.png

Media:Freiburg10 VP2KO.PNG

Media:Freiburg10 VP23Schema.png

Media:Freiburg10_N_terminal_fusion_approach.png

Media:Freiburg10_Cotransfected_plasmids.png

VP1 insertion

Media:Freiburg10_pSB1C3_VP1up_NLS_Fig27.png

Media:Freiburg10_VP1_insertion.png

Targeting molecules

Media:Freiburg10_Nucleotide_sequence_ZEGFR.png

Media:Freiburg10_Darpin.png

Media:Freiburg10_Darpin_internal_repeat_modules.png

Media:Freiburg10_Darpin_conserved_motifs.png

Media:Freiburg10_Darpin_capping_modules.png

Favorite parts

Media:Freiburg10_IMAC.png

Media:Freiburg10_effect_GCV.png

Media:Freiburg10_viral_brick_587_KO_His.png

Media:Freiburg10_psB1C3_ZEGFR_Linker_VP23.png

Media:Freiburg10_Darpin_internal_repeat_modules.png

Media:Freiburg10_Darpin_conserved_motifs.png

Media:Freiburg10_Darpin_capping_modules.png

HSPG KO

Media:Freiburg10 HSPG binding motif.png

Media:Freiburg10 heparansulfate.PNG