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Team:British Columbia/Notebook QS
From 2010.igem.org
(Difference between revisions)
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</div> <!-- end SubWrapper --> | </div> <!-- end SubWrapper --> | ||
| + | <h3>Sonication Protocol (with sonicator)</h3> | ||
| + | <ol> | ||
| + | <li>Add 100uL of sdH2O to 5mg of lysozyme</li> | ||
| + | <li>Pipet this into the cell pellet and resuspend</li> | ||
| + | <li>Add 50uL of 0.5M EDTA pH 8.0 to a final concentration of 25uM</li> | ||
| + | <li>Add 850uL of TE or EB buffer (fill up to 1mL)</li> | ||
| + | <li>Sonicate (ear protectors on!)</li> | ||
| + | <ol type="i"> | ||
| + | <li>Turn sonicator on</li> | ||
| + | <li>Wipe down probe with kimwipe wetted with ethanol</li> | ||
| + | <li>Press start</li> | ||
| + | <li>Turn knob up to desired level (in this case, 5)</li> | ||
| + | <li>Place mirocentrifuge tube under the probe and move it up and down for 15 seconds</li> | ||
| + | <li>Take the tube out and place on ice</li> | ||
| + | <li>Repeat 5 more times</li> | ||
| + | <li>Turn off sonicator</li></ol></ol> | ||
| + | <br></br> | ||
| + | <h3>Sonication Protocol (with lysozyme only)</h3> | ||
</div> <!-- end SubWrapper --> | </div> <!-- end SubWrapper --> | ||
Revision as of 05:56, 27 October 2010
Sonication Protocol (with sonicator)
- Add 100uL of sdH2O to 5mg of lysozyme
- Pipet this into the cell pellet and resuspend
- Add 50uL of 0.5M EDTA pH 8.0 to a final concentration of 25uM
- Add 850uL of TE or EB buffer (fill up to 1mL)
- Sonicate (ear protectors on!)
- Turn sonicator on
- Wipe down probe with kimwipe wetted with ethanol
- Press start
- Turn knob up to desired level (in this case, 5)
- Place mirocentrifuge tube under the probe and move it up and down for 15 seconds
- Take the tube out and place on ice
- Repeat 5 more times
- Turn off sonicator
Sonication Protocol (with lysozyme only)
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OpenWetWare (OWW) is an effort to promote the sharing of information, know-how, and wisdom among researchers and groups who are working in biology & biological engineering. OWW hosts lab/research wikis, course wikis, protocol wikis and wiki blogs.
See our UBC OWW notebook.
