Team:British Columbia/Notebook QS

From 2010.igem.org

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<h3>Sonication Protocol (with sonicator)</h3>
 
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<ol>
 
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<li>Add 100uL of sdH2O to 5mg of lysozyme</li>
 
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<li>Pipet this into the cell pellet and resuspend</li>
 
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<li>Add 50uL of 0.5M EDTA pH 8.0 to a final concentration of 25uM</li>
 
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<li>Add 850uL of TE or EB buffer (fill up to 1mL)</li>
 
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<li>Sonicate (ear protectors on!)</li>
 
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<ol type="i">
 
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<li>Turn sonicator on</li>
 
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<li>Wipe down probe with kimwipe wetted with ethanol</li>
 
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<li>Press start</li>
 
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<li>Turn knob up to desired level (in this case, 5)</li>
 
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<li>Place mirocentrifuge tube under the probe and move it up and down for 15 seconds</li>
 
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<li>Take the tube out and place on ice</li>
 
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<li>Repeat 5 more times</li>
 
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<li>Turn off sonicator</li></ol></ol>
 
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<br></br>
 
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<h3>Sonication Protocol (with lysozyme only)</h3>
 
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Revision as of 05:56, 27 October 2010






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OpenWetWare (OWW) is an effort to promote the sharing of information, know-how, and wisdom among researchers and groups who are working in biology & biological engineering. OWW hosts lab/research wikis, course wikis, protocol wikis and wiki blogs.


See our UBC OWW notebook.

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