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Team:British Columbia/Notebook DspB
From 2010.igem.org
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Revision as of 04:40, 27 October 2010
Sonication Protocol to Lyse Cells
- Add 100uL of sdH2O to 5mg of lysozyme
- Pipet this into the cell pellet and resuspend
- Add 50uL of 0.5M EDTA pH 8.0 to a final concentration of 25uM
- Add 850uL of TE or EB buffer (fill up to 1mL)
- Sonicate (ear protectors on!)
- Turn sonicator on
- Wipe down probe with kimwipe wetted with ethanol
- Press start
- Turn knob up to desired level (in this case, 5)
- Place mirocentrifuge tube under the probe and move it up and down for 15 seconds
- Take the tube out and place on ice
- Repeat 5 more times
- Turn off sonicator
OpenWetWare (OWW) is an effort to promote the sharing of information, know-how, and wisdom among researchers and groups who are working in biology & biological engineering. OWW hosts lab/research wikis, course wikis, protocol wikis and wiki blogs.
See our UBC OWW notebook.
