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| + | {{Template:KIT-Kyoto/menu}} |
| + | <table border=0 width="965px" align="center"><tr><td> |
| + | <div aling="left">[[Team:KIT-Kyoto|Home]] > [[Team:KIT-Kyoto/Note|Notebook]] > [[Team:KIT-Kyoto/Notebook/Promoters|Promoters]]</div></td></tr></table> |
| + | <table border=0 width="965px" align="center"><tr><td valign="top">{{Template:KIT-Kyoto/menu10}} |
| + | </td><td valign="top"><div id="MIGI"> |
| + | == '''各種プロモーター活性''' == |
| + | <div><span style="font-size:10pt;">[https://2010.igem.org/Team:KIT-Kyoto/Notebook-week8#September_29>>retuen to Sept.29<SUP>th</SUP>]</span></div> |
| + | === Contents === |
| + | :[[#k|K121013]] |
| + | :[[#a|ahpC]] |
| + | :[[#s|sufA]] |
| + | :[[#t|tetR]] |
| + | |
| === Data === | | === Data === |
| | | |
Revision as of 09:39, 26 October 2010
![](https://static.igem.org/mediawiki/2010/3/30/KIT-Kyotologo.png)
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各種プロモーター活性
Contents
- K121013
- ahpC
- sufA
- tetR
Data
- K121013
n=1 |
10min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.373 | 0.310 | 0.290 | 0.293 | 0.328 | 0.272 | 0.317 | 0.338 |
0.3720 | 0.3130 | 0.274 | 0.285 | 0.325 | 0.261 | 0.300 | 0.327 |
|
20min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.346 | 0.329 | 0.321 | 0.272 | 0.366 | 0.281 | 0.479 | 0.324 |
0.353 | 0.343 | 0.320 | 0.275 | 0.356 | 0.280 | 0.461 | 0.327 |
|
30min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.327 | 0.289 | 0.331 | 0.356 | 0.352 | 0.353 | 0.344 | 0.474 |
0.339 | 0.293 | 0.333 | 0.365 | 0.358 | 0.368 | 0.340 | 0.467 |
|
40min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.439 | 0.463 | 0.416 | 0.466 | 0.461 | 0.319 | 0.475 | 0.419 |
0.450 | 0.468 | 0.401 | 0.488 | 0.441 | 0.314 | 0.459 | 0.418 |
|
50min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.314 | 0.445 | 0.355 | 0.452 | 0.342 | 0.353 | 0.442 | 0.392 |
0.310 | 0.491 | 0.370 | 0.471 | 0.338 | 0.348 | 0.424 | 0.392 |
|
60min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.468 | 0.478 | 0.459 | 0.459 | 0.446 | 0.354 | 0.515 | 0.420 |
|
70min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.452 | 0.4480 | 0.453 | 0.510 | 0.414 | 0.456 | 0.416 | 0.435 |
0.434 | 0.434 | 0.521 | 0.400 | 0.421 | 0.428 | 0.402 | 0.423 |
|
80min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.439 | 0.458 | 0.450 | 0.417 | 0.510 | 0.248 | 0.401 | 0.428 |
0.427 | 0.430 | 0.426 | 0.413 | 0.477 | 0.248 | 0.401 | 0.432 |
|
K121013 | H2O2 |
| less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
10min | 0.373 | 0.311 | 0.281 | 0.289 | 0.326 | 0.266 | 0.307 | 0.333 |
20min | 0.350 | 0.336 | 0.320 | 0.274 | 0.361 | 0.280 | 0.470 | 0.325 |
30min | 0.333 | 0.291 | 0.332 | 0.361 | 0.355 | 0.361 | 0.342 | 0.471 |
40min | 0.444 | 0.465 | 0.408 | 0.477 | 0.451 | 0.316 | 0.467 | 0.419 |
50min | 0.312 | 0.468 | 0.363 | 0.462 | 0.340 | 0.351 | 0.433 | 0.392 |
60min | 0.468 | 0.478 | 0.459 | 0.459 | 0.446 | 0.354 | 0.515 | 0.420 |
70min | 0.443 | 0.441 | 0.487 | 0.455 | 0.417 | 0.442 | 0.409 | 0.429 |
80min | 0.433 | 0.444 | 0.436 | 0.415 | 0.493 | 0.248 | 0.401 | 0.430 |
|
n=2 |
10min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.324 | 0.282 | 0.327 | 0.300 | 0.329 | 0.301 | 0.306 | 0.362 |
0.324 | 0.286 | 0.315 | 0.299 | 0.320 | 0.306 | 0.306 | 0.367 |
0.321 | 0.294 | 0.313 | 0.300 | 0.327 | 0.310 | 0.315 | 0.367 |
|
20min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.464 | 0.458 | 0.436 | 0.405 | 1.24 | 0.440 | 0.380 | 0.439 |
0.438 | 0.432 | 0.400 | 0.376 | 1.22 | 0.425 | 0.350 | 0.452 |
0.419 | 0.421 | 0.399 | 0.373 | 1.23 | 0.413 | 0.374 | 0.456 |
|
30min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.346 | 0.353 | 0.441 | 0.333 | 0.426 | 0.385 | 0.364 | 0.402 |
0.353 | 0.345 | 0.432 | 0.340 | 0.409 | 0.372 | 0.368 | 0.416 |
0.346 | 0.346 | 0.416 | 0.342 | 0.401 | 0.366 | 0.360 | 0.405 |
|
40min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.399 | 0.461 | 0.413 | 0.541 | 0.416 | 0.395 | 0.510 | 0.372 |
0.370 | 0.461 | 0.396 | 0.524 | 0.403 | 0.377 | 0.506 | 0.377 |
0.368 | 0.450 | 0.397 | 0.54 | 0.396 | 0.377 | 0.480 | 0.379 |
|
50min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.500 | 0.429 | 0.355 | 0.441 | 0.566 | 0.462 | 0.450 | 0.414 |
0.469 | 0.407 | 0.341 | 0.405 | 0.564 | 0.421 | 0.418 | 0.408 |
0.434 | 0.390 | 0.340 | 0.384 | 0.575 | 0.406 | 0.409 | 0.413 |
|
60min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.405 | 0.398 | 0.395 | 0.365 | 0.442 | 0.414 | 0.403 | 0.411 |
0.388 | 0.405 | 0.386 | 0.365 | 0.415 | 0.406 | 0.413 | 0.409 |
0.403 | 0.404 | 0.389 | 0.370 | 0.405 | 0.414 | 0.417 | 0.405 |
|
70min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.426 | 0.600 | 0.557 | 0.413 | 0.454 | 0.450 | 0.434 | 0.387 |
0.426 | 0.545 | 0.504 | 0.421 | 0.454 | 0.462 | 0.429 | 0.393 |
0.436 | 0.506 | 0.500 | 0.431 | 0.465 | 0.466 | 0.446 | 0.404 |
|
80min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.457 | 0.445 | 0.456 | 0.457 | 0.464 | 0.466 | 0.450 | 0.417 |
0.474 | 0.458 | 0.468 | 0.471 | 0.477 | 0.484 | 0.465 | 0.430 |
0.474 | 0.460 | 0.470 | 0.485 | 0.481 | 0.490 | 0.468 | 0.416 |
|
90min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.469 | 0.458 | 0.491 | 0.475 | 0.483 | 0.444 | 0.533 | 0.446 |
0.482 | 0.464 | 0.450 | 0.481 | 0.493 | 0.443 | 0.543 | 0.450 |
0.491 | 0.467 | 0.510 | 0.486 | 0.506 | 0.45 | 0.540 | 0.448 |
|
K121013 | H2O2 |
| less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
10min | 0.323 | 0.287 | 0.318 | 0.299 | 0.326 | 0.306 | 0.309 | 0.365 |
20min | 0.440 | 0.437 | 0.412 | 0.385 | 1.23 | 0.426 | 0.368 | 0.449 |
30min | 0.348 | 0.348 | 0.429 | 0.338 | 0.412 | 0.374 | 0.364 | 0.408 |
40min | 0.468 | 0.409 | 0.345 | 0.410 | 0.568 | 0.430 | 0.425 | 0.412 |
50min | 0.399 | 0.402 | 0.390 | 0.367 | 0.420 | 0.411 | 0.411 | 0.408 |
60min | 0.429 | 0.550 | 0.520 | 0.422 | 0.457 | 0.459 | 0.436 | 0.395 |
70min | 0.469 | 0.455 | 0.465 | 0.471 | 0.474 | 0.480 | 0.461 | 0.421 |
80min | 0.481 | 0.463 | 0.483 | 0.481 | 0.494 | 0.446 | 0.539 | 0.448 |
|
n=3 |
10min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.674 | 0.662 | 0.684 | 0.663 | 0.723 | 0.646 | 0.667 | 0.682 |
0.675 | 0.667 | 0.679 | 0.667 | 0.723 | 0.655 | 0.659 | 0.698 |
0.679 | 0.662 | 0.682 | 0.661 | 0.731 | 0.661 | 0.647 | 0.693 |
|
20min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.715 | 0.704 | 0.712 | 0.705 | 0.695 | 0.696 | 0.768 | 1.06 |
0.715 | 0.690 | 0.698 | 0.706 | 0.694 | 0.695 | 0.753 | 1.04 |
0.714 | 0.708 | 0.705 | 0.699 | 0.709 | 0.701 | 0.736 | 1.04 |
| |
30min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.809 | 0.770 | 0.664 | 0.704 | 0.759 | 0.686 | 0.844 | 0.726 |
0.792 | 0.760 | 0.659 | 0.715 | 0.759 | 0.692 | 0.806 | 0.717 |
0.811 | 0.775 | 0.681 | 0.711 | 0.758 | 0.699 | 0.816 | 0.716 |
|
40min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.768 | 0.729 | 0.713 | 0.704 | 0.800 | 0.790 | 0.776 | 0.742 |
0.766 | 0.726 | 0.703 | 0.710 | 0.796 | 0.788 | 0.769 | 0.750 |
0.772 | 0.731 | 0.707 | 0.717 | 0.802 | 0.785 | 0.773 | 0.759 |
|
50min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.722 | 0.729 | 0.755 | 0.784 | 0.814 | 0.842 | 0.838 | 0.918 |
0.733 | 0.732 | 0.759 | 0.786 | 0.820 | 0.848 | 0.843 | 0.930 |
0.740 | 0.735 | 0.767 | 0.793 | 0.825 | 0.846 | 0.838 | 0.936 |
|
60min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.901 | 0.885 | 0.981 | 0.866 | 0.834 | 0.888 | 0.844 | 0.968 |
0.908 | 0.897 | 0.988 | 0.873 | 0.819 | 0.895 | 0.835 | 0.963 |
0.914 | 0.898 | 0.985 | 0.887 | 0.843 | 0.881 | 0.833 | 0.956 |
|
70min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.942 | 0.985 | 0.967 | 0.917 | 0.914 | 0.910 | 0.970 | 0.932 |
0.941 | 0.972 | 0.955 | 0.900 | 0.911 | 0.897 | 0.940 | 0.931 |
0.942 | 0.966 | 0.957 | 0.900 | 0.903 | 0.879 | 0.953 | 0.941 |
|
80min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
1.04 | 1.01 | 0.994 | 1.02 | 1.03 | 1.01 | 0.995 | 1.17 |
1.02 | 1.01 | 0.987 | 1.01 | 0.969 | 0.995 | 0.974 | 1.14 |
1.03 | 1.02 | 1.01 | 1.00 | 0.976 | 0.999 | 0.979 | 1.14 |
|
90min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
0.979 | 1.01 | 1.05 | 1.02 | 1.04 | 0.989 | 0.933 | 1.12 |
0.997 | 1.01 | 1.06 | 1.01 | 0.995 | 1.01 | 0.951 | 1.11 |
0.982 | 1.01 | 1.07 | 1.04 | 1.02 | 1.00 | 0.949 | 1.10 |
|
K121013 | H2O2 |
| less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
10min | 0.676 | 0.663 | 0.682 | 0.664 | 0.726 | 0.654 | 0.658 | 0.691 |
20min | 0.715 | 0.701 | 0.705 | 0.703 | 0.700 | 0.698 | 0.752 | 1.05 |
30min | 0.804 | 0.768 | 0.668 | 0.710 | 0.759 | 0.693 | 0.822 | 0.720 |
40min | 0.769 | 0.725 | 0.707 | 0.710 | 0.799 | 0.788 | 0.772 | 0.750 |
50min | 0.732 | 0.732 | 0.760 | 0.787 | 0.820 | 0.845 | 0.840 | 0.928 |
60min | 0.942 | 0.974 | 0.960 | 0.905 | 0.909 | 0.895 | 0.955 | 0.935 |
70min | 1.03 | 1.02 | 0.997 | 1.01 | 0.990 | 1.00 | 0.983 | 1.15 |
80min | 0.986 | 1.01 | 1.06 | 1.02 | 1.02 | 0.999 | 0.944 | 1.11 |
90min | 0.986 | 1.01 | 1.06 | 1.03 | 1.02 | 0.999 | 0.944 | 1.11 |
|
- ahpC
n=1 |
10min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
6.36 | 6.55 | 6.64 | 5.95 | 7.19 | 9.85 | 10.4 | 12.0 |
6.48 | 6.71 | 6.73 | 6.11 | 7.34 | 10.2 | 11.0 | 12.4 |
6.52 | 6.72 | 6.78 | 6.15 | 7.46 | 10.4 | 11.3 | 12.8 |
|
20min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
7.82 | 6.91 | 7.31 | 6.92 | 7.26 | 11.4 | 12.6 | 13.6 |
7.89 | 7.04 | 7.47 | 6.97 | 7.37 | 11.4 | 12.9 | 13.9 |
|
30min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
8.17 | 8.20 | 8.62 | 8.71 | 8.97 | 11.3 | 13.6 | 16.1 |
8.32 | 8.30 | 8.79 | 8.83 | 8.98 | 11.4 | 13.9 | 16.1 |
|
40min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
8.37 | 8.75 | 9.20 | 8.52 | 8.86 | 11.5 | 14.9 | 16.0 |
8.39 | 8.73 | 9.19 | 8.55 | 9.05 | 11.9 | 15.3 | 16.2 |
|
50min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
10.3 | 10.5 | 11.5 | 11.1 | 11.9 | 11.1 | 16.6 | 19.8 |
10.5 | 10.8 | 11.8 | 11.5 | 12.3 | 11.7 | 17.2 | 20.2 |
|
60min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
11.9 | 13.3 | 13.0 | 13.3 | 13.8 | 16.0 | 19.4 | 22.5 |
12.1 | 13.7 | 13.3 | 13.5 | 14.0 | 16.3 | 19.8 | 23.1 |
|
70min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
16.7 | 16.0 | 15.8 | 15.7 | 18.0 | 21.2 | 24.2 | 25.0 |
17.2 | 16.6 | 16.4 | 16.0 | 18.5 | 21.5 | 24.9 | 25.2 |
|
80min | less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
21.2 | 27.0 | 22.2 | 23.4 | 23.5 | 25.6 | 28.9 | 31.3 |
22 | 27.7 | 22.8 | 24.1 | 24.1 | 25.7 | 29.1 | 31.6 |
|
ahpC | H2O2 |
| less | 1nM | 10nM | 100nM | 1µM | 10µM | 100µM | 1mM |
10min | 6.45 | 6.66 | 6.72 | 6.07 | 7.33 | 10.2 | 10.9 | 12.4 |
20min | 7.86 | 6.98 | 7.39 | 6.94 | 7.32 | 11.4 | 12.8 | 13.7 |
30min | 8.25 | 8.25 | 8.71 | 8.77 | 8.98 | 11.3 | 13.8 | 16.1 |
40min | 8.38 | 8.74 | 9.20 | 8.53 | 8.95 | 11.7 | 15.1 | 16.1 |
50min | 10.4 | 10.6 | 11.7 | 11.3 | 12.1 | 11.4 | 16.9 | 20.0 |
60min | 12.0 | 13.5 | 13.2 | 13.4 | 13.9 | 16.1 | 19.6 | 22.8 |
70min | 16.9 | 16.3 | 16.1 | 15.9 | 18.3 | 21.3 | 24.6 | 25.1 |
80min | 16.9 | 16.3 | 16.1 | 15.9 | 18.3 | 21.3 | 24.6 | 25.1 |
|
- sufA
- tetR
|