Team:Freiburg Bioware
From 2010.igem.org
Line 40: | Line 40: | ||
</div></li> | </div></li> | ||
<li><div class="news">Number of Glycerolstocks: 763 </div></li> | <li><div class="news">Number of Glycerolstocks: 763 </div></li> | ||
- | <li><div class="news">Test</div></li> | + | <li><div class="news">Number of Biobricks: 116 </div></li> |
+ | <li><div class="news">Number of Glycerolstocks: 763 </div></li> | ||
+ | <li><div class="news">Number of Ordered Oligos: 193 </div></li> | ||
+ | <li><div class="news">Mini-Preps total: 1085</div></li> | ||
+ | <li><div class="news">Midi-Preps total: 106</div></li> | ||
+ | <li><div class="news">Highest Midi-Prep Concentration: 5045,6 ng/µL</div></li> | ||
+ | <li><div class="news">Test</div></li> | ||
<li><div class="news"><img class="right" src="https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png" id="ccc" /><p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><h5>Statistics:</h5></a> | <li><div class="news"><img class="right" src="https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png" id="ccc" /><p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><h5>Statistics:</h5></a> | ||
This Site is in Construction, later you will find more right here...<a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><span class="bold">[more]</span></a></p></div></li> | This Site is in Construction, later you will find more right here...<a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><span class="bold">[more]</span></a></p></div></li> |
Revision as of 23:38, 25 October 2010
![virus (3.5MB)](http://www.molbiotech.uni-freiburg.de/iGEM/wiki2010/images/8/87/Virus_homepage.gif)
Gene delivery using viral vectors holds great promise for the treatment of acquired and inherited diseases. The human Adeno-Associated Virus (AAV) is a small, non-pathogenic, single-stranded DNA virus gaining increasing attention being both versatile and effective. Taking current knowledge into account, we generated a recombinant, modularized, BioBrick-compatible AAV ‘Virus Construction Kit’. We provide parts for modified capsid proteins, targeting modules, tumor-specific promoters, and prodrug-activating enzymes as well as readily assembled vectors for gene delivery and production of non-replicative virus particles. The viral tropism is altered by N-terminal fusion or by loop replacement of the capsid proteins. Functionality of viruses constructed from our kit was demonstrated by fluorescent protein expression in infected cells and by prodrug-induced killing of tumor cells upon viral delivery of a thymidine kinase. Incorporating multiple layers of safety, we provide a general tool to the growing field of personalized medicine and demonstrate its use in tumor therapy.
![](https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png)
Project Results
Highlights:
This Site is in Construction, later you will find more right here...[more]
Heading:
This Site is in Construction, later on you will find more right here...[Klick for more]
Head-Line:
This Site is in Construction, later you will find more right here...[Klick for more]
![](http://www.molbiotech.uni-freiburg.de/iGEM/wiki2010/images/b/b2/Freiburg10_Manual-Logo.png)
Virus Construction Kit - The Manual
Heading:
This site is under construction, later you will find more right here...[more]
Statistics
- Number of Glycerolstocks: 763
- Number of Biobricks: 116
- Number of Glycerolstocks: 763
- Number of Ordered Oligos: 193
- Mini-Preps total: 1085
- Midi-Preps total: 106
- Highest Midi-Prep Concentration: 5045,6 ng/µL
- Test
Statistics:
This Site is in Construction, later you will find more right here...[more]
![](https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png)
BioBricks
Modularisation and Modification of the viral tropismn and the effector function resulted in many different BioBricks.
Click here to explore them.
![](https://static.igem.org/mediawiki/2010/0/0d/Freiburg10_Danger_Virus.png)
Biosafety
The aspect of biological safety was considered well and a risk assessment and security profile for the Adeno-associated Virus were created. To hear about this click here.
![](http://www.molbiotech.uni-freiburg.de/iGEM/wiki2010/images/4/40/Freiburg10_Cuckoo_Clock_Competition_button.png)
Notebook
Head-Line:
This Site is in Construction, later you will find more right here...[Klick for more]
Sponsors
![](https://static.igem.org/mediawiki/2010/4/49/Freiburg10_Sponsoren_Image_Map.gif)