Team:Freiburg Bioware
From 2010.igem.org
Line 84: | Line 84: | ||
<h1>Sponsors</h1> | <h1>Sponsors</h1> | ||
- | + | <div class="map"> | |
<map name="Sponsoren_iGEM" id="Sponsoren_iGEM"> | <map name="Sponsoren_iGEM" id="Sponsoren_iGEM"> | ||
<area shape="circle" coords="705,369,65" href="http://www.uni-potsdam.de/" target="_blank" alt="Uni Potsdam" title="Uni Potsdam" /> | <area shape="circle" coords="705,369,65" href="http://www.uni-potsdam.de/" target="_blank" alt="Uni Potsdam" title="Uni Potsdam" /> | ||
Line 112: | Line 112: | ||
</map> | </map> | ||
<img src="https://static.igem.org/mediawiki/2010/4/49/Freiburg10_Sponsoren_Image_Map.gif" width="826" height="1169" border="0" alt="" title="" usemap="#Sponsoren_iGEM" /> | <img src="https://static.igem.org/mediawiki/2010/4/49/Freiburg10_Sponsoren_Image_Map.gif" width="826" height="1169" border="0" alt="" title="" usemap="#Sponsoren_iGEM" /> | ||
+ | </div> | ||
<br /> | <br /> |
Revision as of 23:30, 25 October 2010
![virus (3.5MB)](http://www.molbiotech.uni-freiburg.de/iGEM/wiki2010/images/8/87/Virus_homepage.gif)
Gene delivery using viral vectors holds great promise for the treatment of acquired and inherited diseases. The human Adeno-Associated Virus (AAV) is a small, non-pathogenic, single-stranded DNA virus gaining increasing attention being both versatile and effective. Taking current knowledge into account, we generated a recombinant, modularized, BioBrick-compatible AAV ‘Virus Construction Kit’. We provide parts for modified capsid proteins, targeting modules, tumor-specific promoters, and prodrug-activating enzymes as well as readily assembled vectors for gene delivery and production of non-replicative virus particles. The viral tropism is altered by N-terminal fusion or by loop replacement of the capsid proteins. Functionality of viruses constructed from our kit was demonstrated by fluorescent protein expression in infected cells and by prodrug-induced killing of tumor cells upon viral delivery of a thymidine kinase. Incorporating multiple layers of safety, we provide a general tool to the growing field of personalized medicine and demonstrate its use in tumor therapy.
![](https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png)
Project Results
Highlights:
This Site is in Construction, later you will find more right here...[more]
Heading:
This Site is in Construction, later on you will find more right here...[Klick for more]
Head-Line:
This Site is in Construction, later you will find more right here...[Klick for more]
![](http://www.molbiotech.uni-freiburg.de/iGEM/wiki2010/images/b/b2/Freiburg10_Manual-Logo.png)
Virus Construction Kit - The Manual
Heading:
This site is under construction, later you will find more right here...[more]
Statistics
- This site is under construction!!
Statistics:
This Site is in Construction, later you will find more right here...[more]
![](https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png)
BioBricks
Modularisation and Modification of the viral tropismn and the effector function resulted in many different BioBricks.
Click here to explore them.
![](https://static.igem.org/mediawiki/2010/0/0d/Freiburg10_Danger_Virus.png)
Biosafety
The aspect of biological safety was considered well and a risk assessment and security profile for the Adeno-associated Virus were created. To hear about this click here.
![](http://www.molbiotech.uni-freiburg.de/iGEM/wiki2010/images/4/40/Freiburg10_Cuckoo_Clock_Competition_button.png)
Notebook
Head-Line:
This Site is in Construction, later you will find more right here...[Klick for more]
Sponsors
![](https://static.igem.org/mediawiki/2010/4/49/Freiburg10_Sponsoren_Image_Map.gif)