Team:KIT-Kyoto/Notebook/Promoters

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(Difference between revisions)

Revision as of 21:14, 25 October 2010

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各種プロモーター活性

>>retuen to Sept.29^th

Data

K121013

n=1
10min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.3734	0.3082	0.2886	0.2934	0.3283	0.2716	0.3172	0.3384
	0.3717	0.3127	0.274	0.2845	0.3245	0.2607	0.2967	0.3267

20min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.3463	0.3286	0.3206	0.272	0.3663	0.2803	0.4792	0.3242
	0.3534	0.3433	0.3197	0.2754	0.3558	0.2799	0.4612	0.3265

30min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.3274	0.2893	0.331	0.3564	0.3519	0.353	0.3443	0.4742
	0.3389	0.2932	0.3331	0.365	0.358	0.368	0.3399	0.4669

40min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.4385	0.4628	0.4159	0.4656	0.4612	0.3186	0.4748	0.4191
	0.4502	0.4679	0.4007	0.488	0.4407	0.3139	0.4587	0.4181

50min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.314	0.4448	0.3551	0.4522	0.3417	0.3533	0.4415	0.3924
	0.3091	0.4911	0.3698	0.4707	0.3383	0.348	0.4238	0.3919

60min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.4679	0.4767	0.4592	0.4591	0.4461	0.3544	0.5154	0.4195

70min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.4521	0.4479	0.4534	0.5095	0.4139	0.4564	0.4158	0.4354
	0.4338	0.4338	0.5212	0.3995	0.4209	0.4283	0.4015	0.4227

80min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.4388	0.4584	0.446	0.417	0.5097	0.2479	0.4006	0.4278
	0.4273	0.4302	0.4259	0.4129	0.4772	0.2475	0.4012	0.4315

K121013	H₂O₂
	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
10min	0.37255	0.31045	0.2813	0.28895	0.3264	0.26615	0.30695	0.33255
20min	0.34985	0.33595	0.32015	0.2737	0.36105	0.2801	0.4702	0.32535
30min	0.33315	0.29125	0.33205	0.3607	0.35495	0.3605	0.3421	0.47055
40min	0.44435	0.46535	0.4083	0.4768	0.45095	0.31625	0.46675	0.4186
50min	0.31155	0.46795	0.36245	0.46145	0.34	0.35065	0.43265	0.39215
60min	0.4679	0.4767	0.4592	0.4591	0.4461	0.3544	0.5154	0.4195
70min	0.44295	0.44085	0.4873	0.4545	0.4174	0.44235	0.40865	0.42905
80min	0.43305	0.4443	0.43595	0.41495	0.49345	0.2477	0.4009	0.42965

n=2

10min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.3239	0.2824	0.327	0.2998	0.3292	0.3009	0.3064	0.3619
	0.3238	0.286	0.3148	0.2993	0.3203	0.3057	0.3064	0.3672
	0.3205	0.2938	0.3125	0.2976	0.327	0.3102	0.3149	0.3667

20min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.4635	0.4575	0.4364	0.4051	1.239	0.4395	0.38	0.4392
	0.4384	0.4318	0.4003	0.376	1.224	0.4249	0.3504	0.4516
	0.4188	0.4213	0.3989	0.3728	1.232	0.4128	0.3738	0.4561

30min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.3459	0.3534	0.4406	0.333	0.4258	0.3852	0.3641	0.4019
	0.3528	0.3454	0.4316	0.3403	0.4087	0.372	0.368	0.4156
	0.3463	0.3463	0.4161	0.3421	0.4005	0.3655	0.3595	0.405

40min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.399	0.4612	0.4131	0.5411	0.4163	0.3952	0.5098	0.3716
	0.3697	0.4614	0.3964	0.5241	0.4031	0.3768	0.5063	0.3774
	0.3678	0.4496	0.3972	0.543	0.3957	0.3772	0.4795	0.379

50min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.5001	0.4291	0.3549	0.4407	0.5656	0.4618	0.4496	0.4137
	0.4694	0.4071	0.3409	0.4045	0.5643	0.4214	0.4179	0.4079
	0.4342	0.3899	0.3389	0.3838	0.5748	0.4058	0.4087	0.4129

60min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.4046	0.3984	0.3951	0.3651	0.4416	0.4142	0.4031	0.4112
	0.3884	0.4048	0.386	0.3649	0.4149	0.4063	0.4132	0.4091
	0.4026	0.4042	0.3889	0.3695	0.4045	0.4136	0.4173	0.4046

70min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.4262	0.5995	0.5567	0.4127	0.4536	0.4496	0.434	0.3871
	0.426	0.5448	0.5036	0.4212	0.4537	0.4617	0.4287	0.3929
	0.4358	0.5057	0.4995	0.4305	0.465	0.4663	0.4464	0.404

80min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.4573	0.4453	0.4563	0.4572	0.4639	0.4662	0.4501	0.4169
	0.4743	0.4582	0.4677	0.4712	0.4772	0.4836	0.4651	0.43
	0.474	0.4602	0.47	0.4847	0.4807	0.4897	0.4682	0.4156

90min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.4692	0.4575	0.4907	0.4748	0.4833	0.4442	0.5329	0.4461
	0.4818	0.4638	0.4496	0.4811	0.4931	0.4431	0.5426	0.4504
	0.491	0.4667	0.5077	0.4855	0.5063	0.452	0.5399	0.4476

K121013	H₂O₂
	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
10min	0.322733333	0.2874	0.3181	0.2989	0.3255	0.3056	0.309233333	0.365266667
20min	0.440233333	0.436866667	0.411866667	0.384633333	1.231666667	0.425733333	0.368066667	0.448966667
30min	0.348333333	0.348366667	0.429433333	0.338466667	0.411666667	0.374233333	0.363866667	0.4075
40min	0.4679	0.4087	0.3449	0.409666667	0.568233333	0.429666667	0.4254	0.4115
50min	0.398533333	0.402466667	0.39	0.3665	0.420333333	0.411366667	0.4112	0.4083
60min	0.429333333	0.55	0.519933333	0.421466667	0.457433333	0.4592	0.436366667	0.394666667
70min	0.468533333	0.454566667	0.464666667	0.471033333	0.473933333	0.479833333	0.461133333	0.420833333
80min	0.480666667	0.462666667	0.482666667	0.480466667	0.494233333	0.446433333	0.538466667	0.448033333

n=3

10min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.6736	0.6615	0.6838	0.6631	0.7225	0.6456	0.6674	0.6824
	0.6747	0.6666	0.6785	0.6668	0.7233	0.6551	0.6593	0.6977
	0.6793	0.6617	0.6822	0.6606	0.7311	0.6608	0.6474	0.6928

20min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.7146	0.7038	0.7116	0.705	0.6954	0.6961	0.7676	1.056
	0.7152	0.6896	0.6982	0.7056	0.694	0.6954	0.7532	1.038
	0.7137	0.7081	0.7047	0.6991	0.7091	0.7012	0.7359	1.043

30min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.8089	0.7698	0.6644	0.7044	0.7587	0.6859	0.8438	0.7259
	0.7919	0.7596	0.6587	0.7152	0.7587	0.6923	0.8064	0.7172
	0.8109	0.7746	0.6814	0.7109	0.7582	0.6993	0.8163	0.7161

40min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.7681	0.7285	0.7127	0.704	0.7995	0.7897	0.776	0.7421
	0.7659	0.7262	0.7025	0.7097	0.7964	0.7878	0.7685	0.7498
	0.7721	0.7307	0.7068	0.7168	0.8021	0.7851	0.7728	0.7586

50min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.7219	0.7292	0.7554	0.7837	0.814	0.8418	0.8384	0.9178
	0.7332	0.7323	0.7592	0.7857	0.82	0.8476	0.8431	0.9303
	0.7401	0.7348	0.7666	0.7929	0.8249	0.8458	0.838	0.9356

60min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.9014	0.8845	0.9814	0.8661	0.8337	0.8881	0.844	0.9678
	0.908	0.8968	0.9881	0.8733	0.8189	0.8946	0.8348	0.9633
	0.9144	0.8982	0.9851	0.8866	0.8431	0.8809	0.8333	0.956

70min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.942	0.9845	0.9667	0.9166	0.9135	0.9099	0.9704	0.9324
	0.9411	0.9721	0.955	0.8998	0.9107	0.8965	0.9403	0.931
	0.9422	0.9663	0.9567	0.8997	0.9032	0.8789	0.9528	0.9409

80min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	1.038	1.013	0.9944	1.024	1.025	1.012	0.9951	1.167
	1.018	1.01	0.9868	1.008	0.9691	0.9953	0.9738	1.14
	1.034	1.024	1.011	1.001	0.9761	0.999	0.9794	1.144

90min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.9791	1.011	1.053	1.023	1.037	0.9886	0.9325	1.116
	0.9971	1.006	1.063	1.013	0.9948	1.007	0.9513	1.108
	0.9818	1.011	1.071	1.038	1.023	1.002	0.9492	1.098

K121013	H₂O₂
	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
10min	0.675866667	0.663266667	0.6815	0.6635	0.725633333	0.653833333	0.658033333	0.690966667
20min	0.7145	0.7005	0.704833333	0.703233333	0.6995	0.697566667	0.752233333	1.045666667
30min	0.8039	0.768	0.668166667	0.710166667	0.758533333	0.6925	0.822166667	0.719733333
40min	0.7687	0.728466667	0.707333333	0.710166667	0.799333333	0.787533333	0.772433333	0.750166667
50min	0.731733333	0.7321	0.7604	0.787433333	0.819633333	0.845066667	0.839833333	0.9279
60min	0.941766667	0.9743	0.959466667	0.905366667	0.909133333	0.8951	0.9545	0.934766667
70min	1.03	1.015666667	0.9974	1.011	0.990066667	1.0021	0.982766667	1.150333333
80min	0.986	1.009333333	1.062333333	1.024666667	1.018266667	0.9992	0.944333333	1.107333333
90min	0.986	1.009333333	1.062333333	1.024666667	1.018266667	0.9992	0.944333333	1.107333333

ahpC

n=1

10min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	6.362	6.549	6.639	5.949	7.186	9.847	10.41	12.03
	6.476	6.705	6.733	6.106	7.341	10.22	10.99	12.44
	6.523	6.723	6.779	6.152	7.458	10.4	11.34	12.78

20min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	7.824	6.91	7.313	6.918	7.257	11.4	12.64	13.56
	7.894	7.043	7.469	6.967	7.373	11.4	12.94	13.86

30min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	8.171	8.195	8.624	8.713	8.967	11.28	13.64	16.13
	8.32	8.302	8.791	8.83	8.983	11.39	13.94	16.11

40min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	8.366	8.753	9.2	8.517	8.855	11.49	14.86	15.97
	8.386	8.733	9.19	8.55	9.053	11.87	15.28	16.2

50min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	10.32	10.47	11.49	11.09	11.93	11.11	16.64	19.77
	10.54	10.79	11.81	11.46	12.32	11.74	17.19	20.18

60min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	11.86	13.29	13.03	13.3	13.81	16.01	19.44	22.54
	12.08	13.65	13.3	13.45	14.04	16.26	19.82	23.05

70min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	16.7	16.02	15.77	15.73	18.03	21.17	24.22	24.95
	17.16	16.61	16.37	16.04	18.46	21.51	24.87	25.23

80min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	21.18	27.04	22.18	23.44	23.54	25.6	28.86	31.32
	22	27.74	22.82	24.1	24.06	25.71	29.14	31.64

ahpC	H₂O₂
	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
10min	6.453666667	6.659	6.717	6.069	7.328333333	10.15566667	10.91333333	12.41666667
20min	7.859	6.9765	7.391	6.9425	7.315	11.4	12.79	13.71
30min	8.2455	8.2485	8.7075	8.7715	8.975	11.335	13.79	16.12
40min	8.376	8.743	9.195	8.5335	8.954	11.68	15.07	16.085
50min	10.43	10.63	11.65	11.275	12.125	11.425	16.915	19.975
60min	11.97	13.47	13.165	13.375	13.925	16.135	19.63	22.795
70min	16.93	16.315	16.07	15.885	18.245	21.34	24.545	25.09
80min	16.93	16.315	16.07	15.885	18.245	21.34	24.545	25.09

sufA

tetR

@@ Line 353: / Line 353: @@
 :[[Image:k3.jpg]]
 :<span id="a">'''ahpC'''</span>
+<TABLE BORDER="0"><TR>
+<TD>n=1</TD></TR>
+<TR>
+<TD><TABLE BORDER="1"><TR>
+<TD ROWSPAN="4">10min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
+<TR>
+<TD>6.362</TD><TD>6.549</TD><TD>6.639</TD><TD>5.949</TD><TD>7.186</TD><TD>9.847</TD><TD>10.41</TD><TD>12.03</TD></TR>
+<TR>
+<TD>6.476</TD><TD>6.705</TD><TD>6.733</TD><TD>6.106</TD><TD>7.341</TD><TD>10.22</TD><TD>10.99</TD><TD>12.44</TD></TR>
+<TR>
+<TD>6.523</TD><TD>6.723</TD><TD>6.779</TD><TD>6.152</TD><TD>7.458</TD><TD>10.4</TD><TD>11.34</TD><TD>12.78</TD></TR>
+</TABLE></TD></TR>
+<TR>
+<TD><TABLE BORDER="1"><TR>
+<TD ROWSPAN="3">20min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
+<TR>
+<TD>7.824</TD><TD>6.91</TD><TD>7.313</TD><TD>6.918</TD><TD>7.257</TD><TD>11.4</TD><TD>12.64</TD><TD>13.56</TD></TR>
+<TR>
+<TD>7.894</TD><TD>7.043</TD><TD>7.469</TD><TD>6.967</TD><TD>7.373</TD><TD>11.4</TD><TD>12.94</TD><TD>13.86</TD></TR>
+</TABLE></TD></TR>
+<TR>
+<TD><TABLE BORDER="1"><TR>
+<TD ROWSPAN="3">30min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
+<TR>
+<TD>8.171</TD><TD>8.195</TD><TD>8.624</TD><TD>8.713</TD><TD>8.967</TD><TD>11.28</TD><TD>13.64</TD><TD>16.13</TD></TR>
+<TR>
+<TD>8.32</TD><TD>8.302</TD><TD>8.791</TD><TD>8.83</TD><TD>8.983</TD><TD>11.39</TD><TD>13.94</TD><TD>16.11</TD></TR>
+</TABLE></TD></TR>
+<TR>
+<TD><TABLE BORDER="1"><TR>
+<TD ROWSPAN="3">40min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
+<TR>
+<TD>8.366</TD><TD>8.753</TD><TD>9.2</TD><TD>8.517</TD><TD>8.855</TD><TD>11.49</TD><TD>14.86</TD><TD>15.97</TD></TR>
+<TR>
+<TD>8.386</TD><TD>8.733</TD><TD>9.19</TD><TD>8.55</TD><TD>9.053</TD><TD>11.87</TD><TD>15.28</TD><TD>16.2</TD></TR>
+</TABLE></TD></TR>
+<TR>
+<TD><TABLE BORDER="1"><TR>
+<TD ROWSPAN="3">50min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
+<TR>
+<TD>10.32</TD><TD>10.47</TD><TD>11.49</TD><TD>11.09</TD><TD>11.93</TD><TD>11.11</TD><TD>16.64</TD><TD>19.77</TD></TR>
+<TR>
+<TD>10.54</TD><TD>10.79</TD><TD>11.81</TD><TD>11.46</TD><TD>12.32</TD><TD>11.74</TD><TD>17.19</TD><TD>20.18</TD></TR>
+</TABLE></TD></TR>
+<TR>
+<TD><TABLE BORDER="1"><TR>
+<TD ROWSPAN="3">60min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
+<TR>
+<TD>11.86</TD><TD>13.29</TD><TD>13.03</TD><TD>13.3</TD><TD>13.81</TD><TD>16.01</TD><TD>19.44</TD><TD>22.54</TD></TR>
+<TR>
+<TD>12.08</TD><TD>13.65</TD><TD>13.3</TD><TD>13.45</TD><TD>14.04</TD><TD>16.26</TD><TD>19.82</TD><TD>23.05</TD></TR>
+</TABLE></TD></TR>
+<TR>
+<TD><TABLE BORDER="1"><TR>
+<TD ROWSPAN="3">70min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
+<TR>
+<TD>16.7</TD><TD>16.02</TD><TD>15.77</TD><TD>15.73</TD><TD>18.03</TD><TD>21.17</TD><TD>24.22</TD><TD>24.95</TD></TR>
+<TR>
+<TD>17.16</TD><TD>16.61</TD><TD>16.37</TD><TD>16.04</TD><TD>18.46</TD><TD>21.51</TD><TD>24.87</TD><TD>25.23</TD></TR>
+</TABLE></TD></TR>
+<TR>
+<TD><TABLE BORDER="1"><TR>
+<TD ROWSPAN="4">80min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
+<TR>
+<TD>21.18</TD><TD>27.04</TD><TD>22.18</TD><TD>23.44</TD><TD>23.54</TD><TD>25.6</TD><TD>28.86</TD><TD>31.32</TD></TR>
+<TR>
+<TD>22</TD><TD>27.74</TD><TD>22.82</TD><TD>24.1</TD><TD>24.06</TD><TD>25.71</TD><TD>29.14</TD><TD>31.64</TD></TR>
+</TABLE></TD></TR>
+<TR>
+<TD><TABLE BORDER="1"><TR>
+<TD>ahpC</TD><TD>H<SUB>2</SUB>O<SUB>2</SUB></TR>
+<TR>
+<TD></TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
+<TR>
+<TD>10min</TD><TD>6.453666667</TD><TD>6.659</TD><TD>6.717</TD><TD>6.069</TD><TD>7.328333333</TD><TD>10.15566667</TD><TD>10.91333333</TD><TD>12.41666667</TD></TR>
+<TR>
+<TD>20min</TD><TD>7.859</TD><TD>6.9765</TD><TD>7.391</TD><TD>6.9425</TD><TD>7.315</TD><TD>11.4</TD><TD>12.79</TD><TD>13.71</TD></TR>
+<TR>
+<TD>30min</TD><TD>8.2455</TD><TD>8.2485</TD><TD>8.7075</TD><TD>8.7715</TD><TD>8.975</TD><TD>11.335</TD><TD>13.79</TD><TD>16.12</TD></TR>
+<TR>
+<TD>40min</TD><TD>8.376</TD><TD>8.743</TD><TD>9.195</TD><TD>8.5335</TD><TD>8.954</TD><TD>11.68</TD><TD>15.07</TD><TD>16.085</TD></TR>
+<TR>
+<TD>50min</TD><TD>10.43</TD><TD>10.63</TD><TD>11.65</TD><TD>11.275</TD><TD>12.125</TD><TD>11.425</TD><TD>16.915</TD><TD>19.975</TD></TR>
+<TR>
+<TD>60min</TD><TD>11.97</TD><TD>13.47</TD><TD>13.165</TD><TD>13.375</TD><TD>13.925</TD><TD>16.135</TD><TD>19.63</TD><TD>22.795</TD></TR>
+<TR>
+<TD>70min</TD><TD>16.93</TD><TD>16.315</TD><TD>16.07</TD><TD>15.885</TD><TD>18.245</TD><TD>21.34</TD><TD>24.545</TD><TD>25.09</TD></TR>
+<TR>
+<TD>80min</TD><TD>16.93</TD><TD>16.315</TD><TD>16.07</TD><TD>15.885</TD><TD>18.245</TD><TD>21.34</TD><TD>24.545</TD><TD>25.09</TD></TR>
+</TABLE></TD></TR></TABLE>
+[[Image:a1.jpg]]

Team:KIT-Kyoto/Notebook/Promoters

From 2010.igem.org

Revision as of 21:14, 25 October 2010

各種プロモーター活性

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