Team:Freiburg Bioware/BioBricks/capsid coding

From 2010.igem.org

(Difference between revisions)
Line 7: Line 7:
-
<!---Basic Capsidcoding Constructs--->
+
<br><br><!---Basic Capsidcoding Constructs--->
<center><h1>Basic Capsidcoding Constructs</h1></center>
<center><h1>Basic Capsidcoding Constructs</h1></center>
<div style="width:935px; height:320px; ">
<div style="width:935px; height:320px; ">
Line 18: Line 18:
height="90px"/></a>
height="90px"/></a>
-
<!--- --->
+
<br><br><!--- --->
<center><h1>Hanna fragen...</h1></center>
<center><h1>Hanna fragen...</h1></center>
<div style="width:965px; height:320px; ">
<div style="width:965px; height:320px; ">
Line 31: Line 31:
-
<!---ViralBricks for Loop replacement--->
+
<br><br><!---ViralBricks for Loop replacement--->
<center><h1>ViralBricks for Loop replacement</h1></center>
<center><h1>ViralBricks for Loop replacement</h1></center>
<div style="width:935px; height:420px; ">
<div style="width:935px; height:420px; ">
Line 85: Line 85:
-
<!---Versions of the Rep-Vp123_p5-TATA-less--->
+
<br><br><!---Versions of the Rep-Vp123_p5-TATA-less--->
<center><h1>Versions of the Rep-Vp123_p5-TATA-less</h1></center>
<center><h1>Versions of the Rep-Vp123_p5-TATA-less</h1></center>
<div style="width:935px; height:320px; ">
<div style="width:935px; height:320px; ">
Line 122: Line 122:
height="110px"/></a>
height="110px"/></a>
-
<!---Versions of the pCMV_Vp123--->
+
<br><br><!---Versions of the pCMV_Vp123--->
<center><h1>Versions of the pCMV_Vp123 </h1></center>
<center><h1>Versions of the pCMV_Vp123 </h1></center>
<div style="width:935px; height:320px; ">
<div style="width:935px; height:320px; ">

Revision as of 10:30, 24 October 2010

Capsid Coding

<parttable>viral_vectors_aav_capsid</parttable>

Basic Capsidcoding Constructs



Hanna fragen...



ViralBricks for Loop replacement

ViralBricks are short nucleotide sequences derived from the gene of the viral coat proteins VP1-3 where they encode the two major surface exposed loops. Within the loop tip functional peptides are integrated that will be present in viral vectors when the ViralBrick was cloned over single cutting restriction sites into the corresponding capsid coding plasmid.

Different ViralBrick versions:

  • 453 integration site
  • 587 integration site
  • 587 integration site with HSPG knock-out

  • Different functional motifs:

  • Biotinylation Acceptor Peptide (BAP)
  • His-Affinity tag (His)
  • RGD-Motif for Integrin targeting
  • Z34C a antibody binding motif from proein A


  • Versions of the Rep-Vp123_p5-TATA-less



    Versions of the pCMV_Vp123