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Team:Freiburg Bioware/BioBricks/capsid coding
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<div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"><p> | <div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"><p> | ||
| - | ViralBricks are short nucleotide sequences derived from the gene of the viral coat proteins VP1-3 where they encode the two major surface exposed loops. Within the loop tip functional peptides are integrated that will be present in viral vectors when the ViralBrick was cloned over single cutting restriction sites into the corresponding capsid coding plasmid. | + | ViralBricks are short nucleotide sequences derived from the gene of the viral coat proteins VP1-3 where they encode the two major surface exposed loops. Within the loop tip functional peptides are integrated that will be present in viral vectors when the ViralBrick was cloned over single cutting restriction sites into the corresponding capsid coding plasmid.<br> |
Different functional motifs: | Different functional motifs: | ||
<li><a href=>Biotinylation Acceptor Peptide (BAP)</a></li> | <li><a href=>Biotinylation Acceptor Peptide (BAP)</a></li> | ||
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<li><a href=>RGD-Motif for Integrin targeting</a></li> | <li><a href=>RGD-Motif for Integrin targeting</a></li> | ||
<li><a href=>Z34C a antibody binding motif from proein A</a></li> | <li><a href=>Z34C a antibody binding motif from proein A</a></li> | ||
| + | Different ViralBrick versions: | ||
| + | <li><a href=>453 integration site</a></li> | ||
| + | <li><a href=>587 integration site</a></li> | ||
| + | <li><a href=>587 integration site with HSPG knock-out</a></li> | ||
| + | |||
</p></div> | </p></div> | ||
Revision as of 10:26, 24 October 2010
Capsid Coding
Basic Capsidcoding Constructs
Hanna fragen...
ViralBricks for Loop replacement
ViralBricks are short nucleotide sequences derived from the gene of the viral coat proteins VP1-3 where they encode the two major surface exposed loops. Within the loop tip functional peptides are integrated that will be present in viral vectors when the ViralBrick was cloned over single cutting restriction sites into the corresponding capsid coding plasmid.
Different functional motifs:
Versions of the Rep-Vp123_p5-TATA-less
Versions of the pCMV_Vp123
