Team:KIT-Kyoto/Protocol
From 2010.igem.org
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<tr><td><span id="tf">'''トランスフォーメーション'''</span></td></tr> | <tr><td><span id="tf">'''トランスフォーメーション'''</span></td></tr> | ||
<tr><td>↓氷上でコンピテント細胞(DH5α:大腸菌株)を融かす</td></tr> | <tr><td>↓氷上でコンピテント細胞(DH5α:大腸菌株)を融かす</td></tr> | ||
- | <tr><td>↓前もって冷やしておいた1.5 mlチューブに100 μlのコンピテント細胞を分注する、余ったコンピテント細胞は-80 ℃の冷凍庫に戻す</ | + | <tr><td>↓前もって冷やしておいた1.5 mlチューブに100 μlのコンピテント細胞を分注する、余ったコンピテント細胞は-80 ℃の冷凍庫に戻す</td></tr> |
- | <tr><td>↓DNAをチューブに1~5 μl加えて、氷上で30分間冷やす</ | + | <tr><td>↓DNAをチューブに1~5 μl加えて、氷上で30分間冷やす</td></tr> |
- | <tr><td>↓42 ℃で45秒間熱ショックを与える</ | + | <tr><td>↓42 ℃で45秒間熱ショックを与える</td></tr> |
- | <tr><td>↓0.9 mlのSOC培地を加える</ | + | <tr><td>↓0.9 mlのSOC培地を加える</td></tr> |
- | <tr><td>↓37 ℃で1時間、振りながら回復培養する</ | + | <tr><td>↓37 ℃で1時間、振りながら回復培養する</td></tr> |
- | <tr><td>↓1 mlをLBプレート(+amp,+kan,+camのいずれか)にまく</ | + | <tr><td>↓1 mlをLBプレート(+amp,+kan,+camのいずれか)にまく</td></tr> |
- | <tr><td>↓37 ℃で一晩培養する</ | + | <tr><td>↓37 ℃で一晩培養する</td></tr> |
</table> | </table> | ||
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<tr><td><span id="tfeng">'''Bacterial transformation'''</span></td></tr> | <tr><td><span id="tfeng">'''Bacterial transformation'''</span></td></tr> | ||
<tr><td>↓Thaw competent cells(DH5 Alpha:E.coli strain) on ice.</td></tr> | <tr><td>↓Thaw competent cells(DH5 Alpha:E.coli strain) on ice.</td></tr> | ||
- | <tr><td>↓Aliquot 100 μl cells into pre-chilled 1.5 ml tube.Put excess competent cells back into the -80℃ freezer.</ | + | <tr><td>↓Aliquot 100 μl cells into pre-chilled 1.5 ml tube.Put excess competent cells back into the -80℃ freezer.</td></tr> |
- | <tr><td>↓Add DNA (1 to 5 μl) to tube, incubate on ice for 30 minutes.</ | + | <tr><td>↓Add DNA (1 to 5 μl) to tube, incubate on ice for 30 minutes.</td></tr> |
- | <tr><td>↓Heat shock the cells at 42 ℃ for 45 seconds. </ | + | <tr><td>↓Heat shock the cells at 42 ℃ for 45 seconds. </td></tr> |
- | <tr><td>↓Add 0.9 ml SOC medium.</ | + | <tr><td>↓Add 0.9 ml SOC medium.</td></tr> |
- | <tr><td>↓Rescue at 37 ℃ for 1 hour, shaking.</ | + | <tr><td>↓Rescue at 37 ℃ for 1 hour, shaking.</td></tr> |
- | <tr><td>↓Spread 1 ml on LB plates(Either +amp,+kan or +cam)</ | + | <tr><td>↓Spread 1 ml on LB plates(Either +amp,+kan or +cam)</td></tr> |
- | <tr><td>↓Clutivate overnight in 37 ℃ .</ | + | <tr><td>↓Clutivate overnight in 37 ℃ .</td></tr> |
</table> | </table> | ||
</td></tr> | </td></tr> | ||
- | <tr><td><div align="right"><span style="font-size:10pt;">[[#Contents|>>Contents]]</span></div> | + | <tr><td> </td><td><div align="right"><span style="font-size:10pt;">[[#Contents|>>Contents]]</span></div> |
</td></tr> | </td></tr> | ||
</table> | </table> | ||
{{Template:KIT-Kyoto-1}} | {{Template:KIT-Kyoto-1}} |
Revision as of 07:20, 23 October 2010
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Language : English / Japanese |
About protocolAs it is of common knowledge, iGEM is carried out within the framework of a protocol common to all teams. Whereas most of the teams are registered on this page, we have uploaded not only the English protocol but also the Japanese one, e.g., our mother tongue version. This is the common protocol with some improvements after we translated it into Japanese.Publishing the Japanese protocol in will be of help to other new Japanese teams in the future. Furthermore, this will be linked to an improvement of iGEM’s name identification in Japan and will enhance the public recognition of synthetic biology. It will also promote Science and Communication among people within not only the scientific but with the non-scientific communities as well. We believe that these improvements will constitute a contribution to the illustration of the public in general.
ContentsProtocol
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