Team:KIT-Kyoto/Protocol/English
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- | <table border=0 width="965px" align="center"><tr><td width="165px" valign="top" align="left"><div> | + | [[Team:KIT-Kyoto|Home]] > [[Team:KIT-Kyoto/Note|Notebook]] > [[Team:KIT-Kyoto/Protocol|Protocol]] > [[Team:KIT-Kyoto/Protocol/English|English]]</td></tr></table> |
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- | </div></td><td width="800px" valign="top" align="left"><div | + | </div></td><td width="800px" valign="top" align="left"><div id="MIGI"> |
== Plasmid Mini Prep == | == Plasmid Mini Prep == | ||
- | + | :*Buffer P1 (Suspension Buffer) | |
::50mM Tris-HCl and 10mM EDTA, pH8.0(25℃)50μg/ml | ::50mM Tris-HCl and 10mM EDTA, pH8.0(25℃)50μg/ml | ||
- | + | :*Buffer P2 (Lysis Buffer) | |
::0.2M NaOH and 1%SDS | ::0.2M NaOH and 1%SDS | ||
- | + | :*Buffer N3 (Neutralization and Binding Buffer) | |
::4M guanidine hydrochloride and 0.5M potassium acetate, pH4.2 | ::4M guanidine hydrochloride and 0.5M potassium acetate, pH4.2 | ||
- | + | :*Buffer PB (Wash Buffer) | |
::5M guanidine hydrochloride,20mM Tris-HCl, pH6.6 (25℃) [final concentrations after addition of ethanol]with 38% ethanol. | ::5M guanidine hydrochloride,20mM Tris-HCl, pH6.6 (25℃) [final concentrations after addition of ethanol]with 38% ethanol. | ||
- | + | :*Buffer PE (Wash Buffer) | |
::20mM NaCl, 2mM Tris-HCl, pH7.5 (25℃) [final concentrations after addition of ethanol:widh 80% ethanol | ::20mM NaCl, 2mM Tris-HCl, pH7.5 (25℃) [final concentrations after addition of ethanol:widh 80% ethanol | ||
- | + | :*Buffer EB(Elution Buffer) | |
- | :10mM Tris-HCl, pH8.5(25℃) | + | ::10mM Tris-HCl, pH8.5(25℃) |
Latest revision as of 08:58, 9 October 2010
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Home > Notebook > Protocol > English |
Plasmid Mini Prep
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