Team:KIT-Kyoto/Protocol/English

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[[Team:KIT-Kyoto|Home]] > [[Team:KIT-Kyoto/Note|Notebook]] > [[Team:KIT-Kyoto/Protocol|Protocol]] > [[Team:KIT-Kyoto/Protocol/English|English]]</td></tr></table>
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== Plasmid Mini Prep ==
== Plasmid Mini Prep ==
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**Buffer P1 (Suspension Buffer)
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:*Buffer P1 (Suspension Buffer)
::50mM Tris-HCl and 10mM EDTA, pH8.0(25℃)50μg/ml
::50mM Tris-HCl and 10mM EDTA, pH8.0(25℃)50μg/ml
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**Buffer P2 (Lysis Buffer)
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:*Buffer P2 (Lysis Buffer)
::0.2M NaOH and 1%SDS
::0.2M NaOH and 1%SDS
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**Buffer N3 (Neutralization and Binding Buffer)
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:*Buffer N3 (Neutralization and Binding Buffer)
::4M guanidine hydrochloride and 0.5M potassium acetate, pH4.2
::4M guanidine hydrochloride and 0.5M potassium acetate, pH4.2
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**Buffer PB (Wash Buffer)
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:*Buffer PB (Wash Buffer)
::5M guanidine hydrochloride,20mM Tris-HCl, pH6.6 (25℃) [final concentrations after addition of ethanol]with 38% ethanol.
::5M guanidine hydrochloride,20mM Tris-HCl, pH6.6 (25℃) [final concentrations after addition of ethanol]with 38% ethanol.
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**Buffer PE (Wash Buffer)
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:*Buffer PE (Wash Buffer)
::20mM NaCl, 2mM Tris-HCl, pH7.5 (25℃) [final concentrations after addition of ethanol:widh 80% ethanol
::20mM NaCl, 2mM Tris-HCl, pH7.5 (25℃) [final concentrations after addition of ethanol:widh 80% ethanol
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**Buffer EB(Elution Buffer)
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:*Buffer EB(Elution Buffer)
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:10mM Tris-HCl, pH8.5(25℃)
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::10mM Tris-HCl, pH8.5(25℃)

Latest revision as of 08:58, 9 October 2010



Home > Notebook > Protocol > English

Plasmid Mini Prep

  • Buffer P1 (Suspension Buffer)
50mM Tris-HCl and 10mM EDTA, pH8.0(25℃)50μg/ml
  • Buffer P2 (Lysis Buffer)
0.2M NaOH and 1%SDS
  • Buffer N3 (Neutralization and Binding Buffer)
4M guanidine hydrochloride and 0.5M potassium acetate, pH4.2
  • Buffer PB (Wash Buffer)
5M guanidine hydrochloride,20mM Tris-HCl, pH6.6 (25℃) [final concentrations after addition of ethanol]with 38% ethanol.
  • Buffer PE (Wash Buffer)
20mM NaCl, 2mM Tris-HCl, pH7.5 (25℃) [final concentrations after addition of ethanol:widh 80% ethanol
  • Buffer EB(Elution Buffer)
10mM Tris-HCl, pH8.5(25℃)



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