Team:Freiburg Bioware/NoteBook/Labjournal/March

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<ul>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/March">March</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/March">March (labday 1)</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April">April</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April">April (labday 2 - 5)</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/May">May</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/May">May (labday 6 - 17)</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/June">June</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/June">June (labday 18 - 45)</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/July">July</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/July">July (labday 46 - 75)</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/August">August</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/August">August part 1 (labday 76 - 92)</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/September">September</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/August2">August part 2 (labday 93 - 106)</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October">October</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/September">September part 1 (labday 107 - 123)</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/November">November</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/September2">September part 2 (labday 124 - 135)</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October">October part 1 (labday 136 - 149 )</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October2">October part 2 (labday 150 - 166 )</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/November">November (labday 167 - 170 )</a></li>
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<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/Cellculture">Cellculture</a></li>
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===<p style="font-size:17px; background-color:#00dd77;">1. Labday 31.03.2010</p>===
===<p style="font-size:17px; background-color:#00dd77;">1. Labday 31.03.2010</p>===
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'''Theoretical cloning'''
'''Theoretical cloning'''
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Sequence analysis (Alignement) of Stratagene Vector with  
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Sequence analysis (Alignement) of the Stratagene Vector with  
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*AAV2
*AAV2
*AAV5
*AAV5
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*AAV8
*AAV8
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'''result:'''  
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'''Result:'''  
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[[Media:AAV_alignment.pdf]]
[[Media:AAV_alignment.pdf]]
<br>
<br>
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Seltsamerweise waren vor allem bei AAV2 einige auffällige Sequenzunterschiede vorhanden, die zu Frameshifts führten. Der Grund hierfür könnte sein, dass die Sequenzdaten von 1983 stammten und deshalb nicht als vollständig richtig eingestuft werden können. Im Zweifelsfall ist es besser sich auf die Sequenz von Stratagene zu verlassen.
 
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Notably, the alignment showed differences in the various AAV2 sequences, some of which are leading to frameshifts. The sequences date from 1983 and might not be completely accurate. We therefore rely on the Stratagene sequence from now on.
Notably, the alignment showed differences in the various AAV2 sequences, some of which are leading to frameshifts. The sequences date from 1983 and might not be completely accurate. We therefore rely on the Stratagene sequence from now on.
'''To do:'''
'''To do:'''
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*download and compare further AAV serotypes. (In Geneious)
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*download and compare further AAV serotypes. (in Geneious)
*compare literature
*compare literature
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*find out possible ways to modify the Capsid
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*find out possible ways to modify the capsid
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<center>[https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April '''=> Go to Labjournal April (labday 2 - 5)''']</center><br><br><br><br>
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Latest revision as of 22:18, 27 October 2010

=> Back to Notebook overview

NoteBook Navigator

1. Labday 31.03.2010

Sequence analysis

Investigators: Christian W., Bea, Kira, Achim, Kerstin, Hanna, Christian L. (Tobias, Kristian)


Theoretical cloning

Sequence analysis (Alignement) of Stratagene Vector with

  • AAV2
  • AAV5
  • AAV7
  • AAV8

Result:

Media:AAV_alignment.pdf
Notably, the alignment showed differences in the various AAV2 sequences, some of which are leading to frameshifts. The sequences date from 1983 and might not be completely accurate. We therefore rely on the Stratagene sequence from now on.

To do:

  • download and compare further AAV serotypes. (in Geneious)
  • compare literature
  • find out possible ways to modify the capsid
=> Go to Labjournal April (labday 2 - 5)




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