Team:Freiburg Bioware/NoteBook/Labjournal/March

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1. Labday 31.03.2010

Sequence analysis

Investigators: Christian W., Bea, Kira, Achim, Kerstin, Hanna, Christian L. (Tobias, Kristian)

Theoretical cloning

Sequence analysis (Alignement) of Stratagene Vector with

AAV2
AAV5
AAV7
AAV8

Result:

Media:AAV_alignment.pdf
Notably, the alignment showed differences in the various AAV2 sequences, some of which are leading to frameshifts. The sequences date from 1983 and might not be completely accurate. We therefore rely on the Stratagene sequence from now on.

To do:

download and compare further AAV serotypes. (in Geneious)
compare literature
find out possible ways to modify the capsid

=> Go to Labjournal April (labday 2 - 5)

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 <ul>
-<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/March">March</a></li>
+<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/March">March (labday 1)</a></li>
-<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April">April</a></li>
+<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April">April (labday 2 - 5)</a></li>
-<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/May">May</a></li>
+<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/May">May (labday 6 - 17)</a></li>
-<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/June">June</a></li>
+<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/June">June (labday 18 - 45)</a></li>
-<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/July">July</a></li>
+<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/July">July (labday 46 - 75)</a></li>
-<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/August">August</a></li>
+<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/August">August part 1 (labday 76 - 92)</a></li>
-<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/September">September</a></li>
+<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/August2">August part 2 (labday 93 - 106)</a></li>
-<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October">October</a></li>
+<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/September">September part 1 (labday 107 - 123)</a></li>
-<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/November">November</a></li>
+<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/September2">September part 2 (labday 124 - 135)</a></li>
+<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October">October part 1 (labday 136 - 149 )</a></li>
+<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October2">October part 2 (labday 150 - 166 )</a></li>
+<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/November">November  (labday 167 - 170 )</a></li>
+<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/Cellculture">Cellculture</a></li>
 </ul>
 </html>
 ===<p style="font-size:17px; background-color:#00dd77;">1. Labday 31.03.2010</p>===
@@ Line 143: / Line 37: @@
 '''Theoretical cloning'''
+Sequence analysis (Alignement) of Stratagene Vector with
-Sequence analysis (Alignement) of the Stratagene Vector with
 *AAV2
 *AAV5
@@ Line 150: / Line 43: @@
 *AAV8
-'''result:'''
+'''Result:'''
 [[Media:AAV_alignment.pdf]]
 <br>
-Curiously there were some significant differences in the aligned sequences of several AAV genomes
+Notably, the alignment showed differences in the various AAV2 sequences, some of which are leading to frameshifts. The sequences date from 1983 and might not be completely accurate. We therefore rely on the Stratagene sequence from now on.
-resulting in frame shifts.
-Maybe because the sequences are from 1983 and therefore incomplete.
-In case of doubt we count on the Stratagene-sequence.
 '''To do:'''
-*download and compare of further AAV serotypes. (In Geneious)
+*download and compare further AAV serotypes. (in Geneious)
 *compare literature
-*find out which possibilities are given to modify the Capsid
+*find out possible ways to modify the capsid
+<center>[https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April '''=> Go to Labjournal April (labday 2 - 5)''']</center><br><br><br><br>
+<!-- End of page-->
 <html>
 </div>
 </html>