Team:Freiburg Bioware
From 2010.igem.org
(10 intermediate revisions not shown) | |||
Line 18: | Line 18: | ||
Structural engineering of the three dimensional viral capsid by introducing four distinct targeting sites led to successful differential transduction of tumor cells. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#targeting"><span class="bold">[more]</span></a></p> | Structural engineering of the three dimensional viral capsid by introducing four distinct targeting sites led to successful differential transduction of tumor cells. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#targeting"><span class="bold">[more]</span></a></p> | ||
<p><!--<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><span class="bold"><h5>Head-Line:</h5></span></a>--> | <p><!--<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><span class="bold"><h5>Head-Line:</h5></span></a>--> | ||
- | Efficient gene-directed enzyme prodrug activation was demonstrated by BioBrick-compatible constructs introduced into tumor cells.<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#arming"><span class="bold">[more]</span></a></p> | + | Efficient gene-directed enzyme prodrug activation was demonstrated by BioBrick-compatible constructs introduced into tumor cells. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#arming"><span class="bold">[more]</span></a></p> |
</div> | </div> | ||
Line 28: | Line 28: | ||
<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><h2>Virus Construction Kit - The Manual</h2></a> | <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><h2>Virus Construction Kit - The Manual</h2></a> | ||
<p><!--<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><h5>Heading:</h5></a>--> | <p><!--<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><h5>Heading:</h5></a>--> | ||
- | Build your own therapeutical virus – 150 pages of knowledge<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><span class="bold">[more]</span></a></p> | + | Build your own therapeutical virus – 150 pages of knowledge. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><span class="bold">[more]</span></a></p> |
</div> | </div> | ||
Line 34: | Line 34: | ||
<div class="box_long box_long_news news_ticker"> | <div class="box_long box_long_news news_ticker"> | ||
- | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Statistics"><h2>Statistics - In a Minute</h2></a> | + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Statistics" style="z-index:20;position:relative;"><h2>Statistics - In a Minute</h2></a> |
<ul id="news" class="newsticker"> | <ul id="news" class="newsticker"> | ||
<li><div class="news"><img class="right" src="https://static.igem.org/mediawiki/2010/0/0b/Freiburg10_Number_of_Plasmids.png" id="ccc" /><p> | <li><div class="news"><img class="right" src="https://static.igem.org/mediawiki/2010/0/0b/Freiburg10_Number_of_Plasmids.png" id="ccc" /><p> | ||
Line 81: | Line 81: | ||
<a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><img class="right" src="https://static.igem.org/mediawiki/2010/d/d1/Freiburg_10_BioBrick_icon_small.png" id="ccc" /></a> | <a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><img class="right" src="https://static.igem.org/mediawiki/2010/d/d1/Freiburg_10_BioBrick_icon_small.png" id="ccc" /></a> | ||
<a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><h2>BioBricks</h2></a> | <a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><h2>BioBricks</h2></a> | ||
- | <p class="standard">Modularization and modification of the viral capsids for retargeting approaches and directed gene delivery of suicide genes resulted in many different BioBricks.<br> Click here to explore them.</p> | + | <p class="standard">Modularization and modification of the viral capsids for retargeting approaches and directed gene delivery of suicide genes resulted in many different BioBricks.<br> Click here to explore them. <a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><span class="bold">[more]</a></span></p> |
</div> | </div> | ||
Line 88: | Line 88: | ||
<a href="https://2010.igem.org/Team:Freiburg_Bioware/Safety"><img class="right" src="https://static.igem.org/mediawiki/2010/f/f9/Freiburg10_Danger_Virus_small.png" id="ccc" /></a> | <a href="https://2010.igem.org/Team:Freiburg_Bioware/Safety"><img class="right" src="https://static.igem.org/mediawiki/2010/f/f9/Freiburg10_Danger_Virus_small.png" id="ccc" /></a> | ||
<a href="https://2010.igem.org/Team:Freiburg_Bioware/Safety"><h2>Biosafety</h2></a> | <a href="https://2010.igem.org/Team:Freiburg_Bioware/Safety"><h2>Biosafety</h2></a> | ||
- | <p class="standard"> | + | <p class="standard">Aspects of biological safety were considered throughout and a risk assessment and security profile of the Adeno-associated Virus were created. Read more about Biosafety issues. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Safety"><span class="bold">[more]</span></a></p> |
- | + | ||
</div> | </div> | ||
Line 99: | Line 98: | ||
<h2>Notebook</h2></a> | <h2>Notebook</h2></a> | ||
<p> | <p> | ||
- | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook"><span class="bold"> | + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook"><span class="bold">From DNA to virus particle to cell culture? </span></a><br /> |
- | + | Yes! Details on cloning, cell culture, flow cytometry analyses, quantifications by ELISA or Western Blot, cytotoxicity assays, time resolved microscopy ... <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Methods"><span class="bold">[more]</span></a></p> | |
- | + | ||
- | + | ||
</div> | </div> | ||
Line 112: | Line 109: | ||
<a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Team_2010"> | <a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Team_2010"> | ||
<h2>Team</h2></a> | <h2>Team</h2></a> | ||
- | <p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Team_2010">< | + | <p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Team_2010"><span class="bold">Fun and Science at the same time?</span></a></a><br /> |
- | Yes! The iGEM team Freiburg did not only share the lab bench, but as well some nice days <a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Photo_Gallery"><span class="bold">canoeing</span></a> and initiated the photo contest <a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Cuckoo_Clock"><span class="bold"> | + | Yes! The iGEM team Freiburg did not only share the lab bench, but as well some nice days <a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Photo_Gallery"><span class="bold">canoeing</span></a> and initiated the photo contest Cuckoo Clock Competition. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Cuckoo_Clock"><span class="bold">[more]</span></a></p> |
</div> | </div> | ||
</div> | </div> | ||
Line 125: | Line 122: | ||
<area shape="circle" coords="156,138,121" href="http://www.uni-freiburg.de/start-en.html?set_language=en" target="_blank" alt="Uni Freiburg" title="Uni Freiburg" /> | <area shape="circle" coords="156,138,121" href="http://www.uni-freiburg.de/start-en.html?set_language=en" target="_blank" alt="Uni Freiburg" title="Uni Freiburg" /> | ||
<area shape="rect" coords="304,8,815,269" href="http://www.bioss.uni-freiburg.de/cms/index.php#" target="_blank" alt="Bioss" title="Bioss" /> | <area shape="rect" coords="304,8,815,269" href="http://www.bioss.uni-freiburg.de/cms/index.php#" target="_blank" alt="Bioss" title="Bioss" /> | ||
- | <area shape="rect" coords="501,311,611,434" href="http://www. | + | <area shape="rect" coords="501,311,611,434" href="http://www.syntbio.net/" target="_blank" alt="kuk.lab" title="kuk.lab" /> |
<area shape="rect" coords="274,329,480,412" href="http://www.daad.de/en/index.html" alt="daad" target="_blank" title="daad" /> | <area shape="rect" coords="274,329,480,412" href="http://www.daad.de/en/index.html" alt="daad" target="_blank" title="daad" /> | ||
<area shape="rect" coords="67,324,238,419" href="http://www.frias.uni-freiburg.de/" alt="frias" target="_blank" title="frias" /> | <area shape="rect" coords="67,324,238,419" href="http://www.frias.uni-freiburg.de/" alt="frias" target="_blank" title="frias" /> | ||
Line 153: | Line 150: | ||
<center><a href="http://www2.clustrmaps.com/counter/maps.php?url=https://2010.igem.org/Team:Freiburg_Bioware" id="clustrMapsLink"><img src="http://www2.clustrmaps.com/counter/index2.php?url=https://2010.igem.org/Team:Freiburg_Bioware" style="border:0px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com';" /> | <center><a href="http://www2.clustrmaps.com/counter/maps.php?url=https://2010.igem.org/Team:Freiburg_Bioware" id="clustrMapsLink"><img src="http://www2.clustrmaps.com/counter/index2.php?url=https://2010.igem.org/Team:Freiburg_Bioware" style="border:0px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com';" /> | ||
</a></center> | </a></center> | ||
+ | <br /> | ||
+ | <center><small>primary team contact: Kristian M. Müller; <a href="mailto:Kristian@syntbio.net">e-mail: Kristian@syntbio.net</a></small></center> | ||
</html> | </html> | ||
{{:Team:Freiburg_Bioware/Footer}} | {{:Team:Freiburg_Bioware/Footer}} |
Latest revision as of 15:23, 14 January 2011
![virus (3.5MB)](https://static.igem.org/mediawiki/2010/2/24/Freiburg10_rot250trans.gif)
Gene delivery using viral vectors holds great promise for the treatment of acquired and inherited diseases. The human Adeno-Associated Virus (AAV) is a small, non-pathogenic, single-stranded DNA virus gaining increasing attention being both versatile and effective. Taking current knowledge into account, we generated a recombinant, modularized, BioBrick-compatible AAV ‘Virus Construction Kit’. We provide parts for modified capsid proteins, targeting modules, tumor-specific promoters, and prodrug-activating enzymes as well as readily assembled vectors for gene delivery and production of non-replicative virus particles. The viral tropism is altered by N-terminal fusion or by loop replacement of the capsid proteins. Functionality of viruses constructed from our kit was demonstrated by fluorescent protein expression in infected cells and by prodrug-induced killing of tumor cells upon viral delivery of a thymidine kinase. Incorporating multiple layers of safety, we provide a general tool to the growing field of personalized medicine and demonstrate its use in tumor therapy.
![](https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png)
Project Results
Team Freiburg presents a functional, modular Virus Construction Kit for specifically targeting and killing tumor cells. [more]
Structural engineering of the three dimensional viral capsid by introducing four distinct targeting sites led to successful differential transduction of tumor cells. [more]
Efficient gene-directed enzyme prodrug activation was demonstrated by BioBrick-compatible constructs introduced into tumor cells. [more]
![](https://static.igem.org/mediawiki/2010/f/fb/Freiburg10_Manual_Logo_small.png)
Virus Construction Kit - The Manual
Build your own therapeutical virus – 150 pages of knowledge. [more]
Statistics - In a Minute
What happened in the lab during iGEM?
Longest Wikipage:
with 222,795 bytesNumber of Biobricks:
116Number of Ordered Oligos:
193Mini-Preps total:
1085Midi-Preps total:
106Highest Midi-Prep Concentration:
5045,6 ng/µLNumber of Glycerolstocks:
763Number of Team members:
15Days in the lab:
170Longest Labday:
18hCoffee Consumed During iGEM:
2238 Cups
![](https://static.igem.org/mediawiki/2010/d/d1/Freiburg_10_BioBrick_icon_small.png)
BioBricks
Modularization and modification of the viral capsids for retargeting approaches and directed gene delivery of suicide genes resulted in many different BioBricks.
Click here to explore them. [more]
![](https://static.igem.org/mediawiki/2010/f/f9/Freiburg10_Danger_Virus_small.png)
Biosafety
Aspects of biological safety were considered throughout and a risk assessment and security profile of the Adeno-associated Virus were created. Read more about Biosafety issues. [more]
![](https://static.igem.org/mediawiki/2010/9/95/Freiburg_10_Notebook_image_small.png)
Notebook
From DNA to virus particle to cell culture?
Yes! Details on cloning, cell culture, flow cytometry analyses, quantifications by ELISA or Western Blot, cytotoxicity assays, time resolved microscopy ... [more]
![](https://static.igem.org/mediawiki/2010/b/bd/Freiburg10_Virus_Logo_Small_small.png)
Team
Fun and Science at the same time?
Yes! The iGEM team Freiburg did not only share the lab bench, but as well some nice days canoeing and initiated the photo contest Cuckoo Clock Competition. [more]
Sponsors
![](https://static.igem.org/mediawiki/2010/4/49/Freiburg10_Sponsoren_Image_Map.gif)