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Finally we successfully combined different modifactions of the therapeutic viral vector for optimizing it for tumore therapy application. These modifications include the knock down of the natural tropism for HSPG receptor, the tumor targeting using the affibody ZEGFR1907 fused to the N-terminus of the viral coat protein VP2 and an encapsidated vectorgenome that was bricked down and reassembled containing the prodrug converting Guanosine Monophosphate Kinase Thymidine Kinase fusion protein (mGMK-TK).

Contents 1 AAAAAH 2 eGFP 3 Time lapse 4 Structure Modeling 5 Modularaization of GOI - Results 6 Introduction to Adeno-Associated Virus Serotype 2 7 Trafficking 8 Helper genes 9 Gene therapy 10 Cytosine deaminase 11 Loop insertions 12 Tagging 13 Targeting 14 N-terminal fusion 14.1 Results 15 VP1 insertion 16 Targeting molecules 17 Favorite parts 18 HSPG KO 19 ITR Diary 20 Targeting: Flow Cytometry: DARPin, Affi (HSPGKO)

AAAAAH

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eGFP

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Time lapse

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Structure Modeling

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Modularaization of GOI - Results

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Introduction to Adeno-Associated Virus Serotype 2

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Trafficking

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Helper genes

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Gene therapy

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Cytosine deaminase

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Loop insertions

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Tagging

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Targeting

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N-terminal fusion

Results

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VP1 insertion

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Targeting molecules

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Favorite parts

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HSPG KO

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ITR Diary

Targeting: Flow Cytometry: DARPin, Affi (HSPGKO)

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