File list 2

Contents 1 Arming 2 3.Material and Method 3 4.Transduction 4 5.statistics_bilder 5 qPCR 6 Modularization: RepCap

Arming

Media:Freiburg10 BBA overview suicide.png

Media:Freiburg10 Cloning mGMK TK30 Arming.png

Media:Freiburg10 Diagram mGMK effectFACS.png

Media:Freiburg10 FACS A D.png

Media:Freiburg10 FACS E H.png

Media:Freiburg10 CD ToxicEffect.png

Media:Freiburg10 CD Bystander.png

Media:Freiburg10 Microscopy Arming A.png

Media:Freiburg10 Microscopy Arming B.png

Media:Freiburg10 Microscopy Arming C.png

Media:Freiburg10 Microscopy Arming D.png

Media:Freiburg10 MTT 72h 2d.png

Media:Freiburg10 MTT 72h 3d.png

Media:Freiburg10 MTT 96h 2d.png

Media:Freiburg10 MTT 96h 3d.png

Media:Freiburg10 SDM mGMK TK30.png

3.Material and Method

3.1

Media:Freiburg10_biobrick_assambly_stratagy.png

Media:Freiburg10_fill_in_reaction.png

Media:Freiburg10_GeneRule_DNA_ladder_mix.png

3.2

Media:Freiburg10_Schematic_overview_flow_cytometer.png

Media:Freiburg10_light-scattering.png

Media:Freiburg10_Excitation_emission_spectra.png

Media:Freiburg10_Laser_light_source.png

Media:Freibur10_gating_for_excluding_cell_debris.png

Media:Freiburg10_histogram_negative_control.png

Media:Freiburg10_histogram_negative_control_II.png

Media:Freiburg10_MTT_reaction.png

Media:Freiburg10_MTT_method.png

Media:Freiburg10_fluor_luoresc.png

Media:Freiburg10_genomic_titer.png

Media:Freiburg10_infectious_titer.png

Media:Freiburg10_PCR_program.png

4.Transduction

Media:Freiburg10_transduction_CMV_mVenus_1.png

Media:Freiburg10_transduction_CMV_mVenus_2.png

Media:Freiburg10_transduction_CMV_mVenus_3.png

Media:Freiburg10_transduction_CMV_mVenus_4.png

Media:Freiburg10_transduction_CMV_mVenus_2.png

Media:Freiburg10_transduction_CMV_mVenus_3.png

5.statistics_bilder

Media:Freiburg10_Days in the lab.png

Media:Freiburg10_Number of Team members.png

Media:Freiburg10_Number of Biobricks.png

Media:Freiburg10_Number of Celllines.png

Media:Freiburg10_Number of Glyzerolstocks.png

Media:Freiburg10_Highest Prep concentration.png

Media:Freiburg10_Coffee consumed during iGEM.png

Media:Freiburg10_Longest Labday.png

Media:Freiburg10_Longest Wikipage.png

Media:Freiburg10_Midipreps total.png

Media:Freiburg10_Minipreps total.png

Media:Freiburg10_Number of ordered Oligo-Nucleotides.png

Media:Freiburg10_Number of Plasmids.png

qPCR

[http://partsregistry.org/Image:Freiburg10_inf_titer_RGD_587.png Freiburg10 inf titer RGD 587.png]

Modularization: RepCap

Media:Freiburg10 pAAV complete mutations.png

Media:Freiburg10 rep synthetic gene fragment.png

Media:Freiburg10 cap synthetic gene fragment.png

Media:Freiburg10 wt vs 4xmutant.png

Media:Freiburg10 facs a.png

Media:Freiburg10 facs b.png

Media:Freiburg10 facs c.png

Media:Freiburg10 facs d.png

Media:Freiburg10 facs e.png

Media:Freiburg10 facs f.png

Media:Freiburg10 facs g.png

Media:Freiburg10 facs h.png

Media:Freiburg10 facs i.png

Media:Freiburg10 facs j.png

Media:Freiburg10 KpnI deleted.png

Media:Freiburg10 KpnI reinstated.png

Media:Freiburg10_pSB1C3_001_mutations.png

Media:Freiburg10 pAAV pSB1C3 001.png

Media:Freiburg10_HSPG-ko_sequence.png

Media:Freiburg10_columns_HSPG_HSPG_ko.png

Media:Freiburg10 qPCR HSPG HSPG ko.png

Media:Freiburg10_Freiburg10_facs_table1.png

Media:Freiburg10_Freiburg10_facs_table2.png

Media:Freiburg10 HSPG-ko redone.png

Media:Freiburg10 RepCap HSPG-ko p5TATAless.png

Media:Freiburg10 HSPG-ko redoneII.png