Team:Freiburg Bioware

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<div class="virus"><img src="http://www.molbiotech.uni-freiburg.de/iGEM/wiki2010/images/8/87/Virus_homepage.gif"  title="virus (3.5MB)" id="animated_virus"/></div>
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<div class="virus"><img src="https://static.igem.org/mediawiki/2010/2/24/Freiburg10_rot250trans.gif"  title="virus (3.5MB)" id="animated_virus"/></div>
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<!---Box on the upper left: Project Summary--->
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<!---Box on the upper left: Project Results--->
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<div class="box_home">
<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project"><img class="right" src="https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png" id="ccc" />
<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project"><img class="right" src="https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png" id="ccc" />
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<h2>Project Results</h2>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results"><h2>Project Results</h2></a>
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<p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#highlights"><h5>Highlights: </h5></a>
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<p><!--<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#highlights"><h5>Highlights: </h5></a>-->
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This Site is in Construction, later you will find more right here...<a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><span class="bold">[more]</span></a></p>
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Team Freiburg presents a functional, modular Virus Construction Kit for specifically targeting and killing tumor cells. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#highlights"><span class="bold">[more]</span></a></p>
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<p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><span class="bold"><h5>Heading:</h5></span></a>
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<p><!--<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#highlights"><span class="bold"><h5>Heading:</h5></span></a>-->
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This Site is in Construction, later on you will find more right here...<a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><span class="bold">[Klick for more]</span></a></p>
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Structural engineering of the three dimensional viral capsid by introducing four distinct targeting sites led to successful differential transduction of tumor cells. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#targeting"><span class="bold">[more]</span></a></p>
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<p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><span class="bold"><h5>Head-Line:</h5></span></a>
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<p><!--<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><span class="bold"><h5>Head-Line:</h5></span></a>-->
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This Site is in Construction, later you will find more right here...<a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><span class="bold">[Klick for more]</span></a></p>
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Efficient gene-directed enzyme prodrug activation was demonstrated by BioBrick-compatible constructs introduced into tumor cells. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#arming"><span class="bold">[more]</span></a></p>
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<img class="right" src="http://www.molbiotech.uni-freiburg.de/iGEM/wiki2010/images/b/b2/Freiburg10_Manual-Logo.png" id="ccc" />
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit">
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<h2>Virus Construction Kit - The Manual</h2>
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<img class="right" src="https://static.igem.org/mediawiki/2010/f/fb/Freiburg10_Manual_Logo_small.png" id="ccc" /></a>
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<p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><h5>Heading:</h5></a>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><h2>Virus Construction Kit - The Manual</h2></a>
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This site is under construction, later you will find more right here...<a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><span class="bold">[more]</span></a></p>
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<p><!--<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><h5>Heading:</h5></a>-->
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Build your own therapeutical virus – 150 pages of knowledge. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><span class="bold">[more]</span></a></p>
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</div>
<!--- News Ticker --->
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<div class="box_long box_long_news news_ticker">
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<h2>Statistics</h2>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Statistics" style="z-index:20;position:relative;"><h2>Statistics - In a Minute</h2></a>
<ul id="news" class="newsticker">
<ul id="news" class="newsticker">
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   <li><div class="news"><img class="right" src="https://static.igem.org/mediawiki/2010/0/0b/Freiburg10_Number_of_Plasmids.png" id="ccc" /><p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><h5>In a minute</h5></a>
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   <li><div class="news"><img class="right" src="https://static.igem.org/mediawiki/2010/0/0b/Freiburg10_Number_of_Plasmids.png" id="ccc" /><p>
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What happened in the last eight month during iGEM<a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><span class="bold">[more]</span></a></p>
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What happened in the lab during iGEM? </p>
</div></li>
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<li><div class="news">Longest Wikipage: “‎Team:Freiburg Bioware/NoteBook/Labjournal/October” with 222,795 bytes</div>
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<li><div class="news"><img
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src="https://static.igem.org/mediawiki/2010/a/a5/Freiburg10_Longest_Wikipage.png"
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alt="Longest Wikipage" width="368"><p>Longest Wikipage:<br />with 222,795 bytes</p></div>
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</li>
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<li><div class="news">Number of Biobricks: 116 </div></li>
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<li><div class="news"><img src="https://static.igem.org/mediawiki/2010/1/10/Freiburg10_Number_of_Biobricks.png"
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<li><div class="news">Number of Ordered Oligos: 193 </div></li>
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alt="Number of BioBricks" width="368"><p>Number of Biobricks:<br /> 116</p></div></li>
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<li><div class="news">Mini-Preps total: 1085</div></li>
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<li><div class="news"><img heigth="100px" src="https://static.igem.org/mediawiki/2010/3/3f/Freiburg10_Number_of_ordered_Oligo-Nucleotides.png"
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<li><div class="news">Midi-Preps total: 106</div></li>
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alt="Number of ordered Oligo-Nucleotides" width="184"><p>Number of Ordered Oligos:<br /> 193</p></div></li>
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<li><div class="news">Highest Midi-Prep Concentration: 5045,6 ng/µL</div></li>
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<li><div class="news"><img
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<li><div class="news">Number of Glycerolstocks: 763 </div></li>
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src="https://static.igem.org/mediawiki/2010/f/f3/Freiburg10_Minipreps_total.png"
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<li><div class="news">Number of Team members: 16</div></li>
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alt="Minipreps total" width="184"><p>Mini-Preps total:<br /> 1085</p></div></li>
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<li><div class="news">Days in the lab: 170</div></li>
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<li><div class="news"><img
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<li><div class="news">Longest Labday: 18h</div></li>
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src="https://static.igem.org/mediawiki/2010/f/f7/Freiburg10_Midipreps_total.png"
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<li><div class="news">Coffee Consumed During iGEM : 2238 Cups</div></li>
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alt="Midipreps total" width="368"><p>Midi-Preps total:<br /> 106</p></div></li>
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  <li><div class="news"><img class="right" src="https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png" id="ccc" /><p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><h5>Statistics:</h5></a>
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<li><div class="news"><img
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What happened during the last eight month...<a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><span class="bold">[more]</span></a></p></div></li>
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src="https://static.igem.org/mediawiki/2010/d/d6/Freiburg10_Highest_Prep_concentration.png"
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alt="Highest Prep concentration (Midi)" width="368"><p>Highest Midi-Prep Concentration:<br />5045,6 ng/µL </p></div></li>
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<li><div class="news"><img
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src="https://static.igem.org/mediawiki/2010/a/af/Freiburg10_Number_of_Glyzerolstocks.png"
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alt="Number of Glycerolstocks" width="368"><p>Number of Glycerolstocks:<br /> 763 </p></div></li>
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<li><div class="news"><img
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src="https://static.igem.org/mediawiki/2010/6/60/Freiburg10_Number_of_Team_members.png"
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alt="Number of Team members" width="368"><p>Number of Team members:<br /> 15 </p></div></li>
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<li><div class="news"><img
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src="https://static.igem.org/mediawiki/2010/0/03/Freiburg10_Days_in_the_lab.png"
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alt="Days in the lab" width="368"><p>Days in the lab:<br /> 170 </p></div></li>
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<li><div class="news"><img
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src="https://static.igem.org/mediawiki/2010/d/d1/Freiburg10_Longest_Labday.png"
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alt="Longest Labday" width="368"><p>Longest Labday:<br />18h </p></div></</li>
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<li><div class="news"><img height="100px"
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src="https://static.igem.org/mediawiki/2010/b/b5/Freiburg10_Coffee_consumed_during_iGEM.png"
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alt="Coffee consumed during iGEM" width="184"><p>Coffee Consumed During iGEM:<br /> 2238 Cups</p></div></li>
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<li><div class="news"><img heigth="100px"class="right" src="https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png" id="ccc" /><p><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Statistics"><h5>!Freiburg Bioware Statistics!</h5></a></p></div></li>
</ul>
</ul>
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<!---Box on the upper right: BioBricks--->
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<div class="box_home_small">
<div class="box_home_small">
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><img class="right" src="https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png" id="ccc" /></a>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><img class="right" src="https://static.igem.org/mediawiki/2010/d/d1/Freiburg_10_BioBrick_icon_small.png" id="ccc" /></a>
<a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><h2>BioBricks</h2></a>
<a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><h2>BioBricks</h2></a>
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<p class="standard">Modularisation and Modification of the viral tropismn and the effector function resulted in many different BioBricks.<br> Click here to explore them.</p>
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<p class="standard">Modularization and modification of the viral capsids for retargeting approaches and directed gene delivery of suicide genes resulted in many different BioBricks.<br> Click here to explore them. <a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><span class="bold">[more]</a></span></p>
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<!---Box on the lower left: Biosafety--->
<!---Box on the lower left: Biosafety--->
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Human_Practice/Biosafety"><img class="right" src="https://static.igem.org/mediawiki/2010/0/0d/Freiburg10_Danger_Virus.png" id="ccc" /></a>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Safety"><img class="right" src="https://static.igem.org/mediawiki/2010/f/f9/Freiburg10_Danger_Virus_small.png" id="ccc" /></a>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Human_Practice/Biosafety"><h2>Biosafety</h2></a>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Safety"><h2>Biosafety</h2></a>
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<p class="standard">The aspect of biological safety was considered well and a risk assessment and security profile for the Adeno-associated Virus were created. To hear about this click here.</p>
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<p class="standard">Aspects of biological safety were considered throughout and a risk assessment and security profile of the Adeno-associated Virus were created. Read more about Biosafety issues. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Safety"><span class="bold">[more]</span></a></p>
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<!---Box on the lower right: Notebook--->
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Human_Practice/Biosafety"><div class="box_home_small">
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<img class="right" src="http://www.molbiotech.uni-freiburg.de/iGEM/wiki2010/images/4/40/Freiburg10_Cuckoo_Clock_Competition_button.png" id="ccc" /></a>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook">
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Cuckoo Clock">
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<img class="right" src="https://static.igem.org/mediawiki/2010/9/95/Freiburg_10_Notebook_image_small.png" id="ccc" /></a>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook">
<h2>Notebook</h2></a>
<h2>Notebook</h2></a>
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<p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><span class="bold"><h5>Head-Line:</h5></span></a>
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<p>
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This Site is in Construction, later you will find more right here...<a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><span class="bold">[Klick for more]</span></a></p>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook"><span class="bold">From DNA to virus particle to cell culture? </span></a><br />
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</a>
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Yes! Details on cloning, cell culture, flow cytometry analyses, quantifications by ELISA or Western Blot, cytotoxicity assays, time resolved microscopy ... <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Methods"><span class="bold">[more]</span></a></p>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Team_2010">
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Team">
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<img class="right" src="https://static.igem.org/mediawiki/2010/b/bd/Freiburg10_Virus_Logo_Small_small.png" id="ccc" /></a>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Team_2010">
<h2>Team</h2></a>
<h2>Team</h2></a>
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<p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><h5>Heading:</h5></a></a>
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<p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Team_2010"><span class="bold">Fun and Science at the same time?</span></a></a><br />
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This Site is in Construction, later you will find more right here...<a href="https://2010.igem.org/Team:Freiburg_Bioware/Virus_Construction_Kit"><span class="bold">[Klick for more]</span></a></p>
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Yes! The iGEM team Freiburg did not only share the lab bench, but as well some nice days <a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Photo_Gallery"><span class="bold">canoeing</span></a> and initiated the photo contest Cuckoo Clock Competition. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Cuckoo_Clock"><span class="bold">[more]</span></a></p>
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<br />
<h1>Sponsors</h1>
<h1>Sponsors</h1>
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<div class="map">
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<div class="map" height="1169px">
<map name="Sponsoren_iGEM" id="Sponsoren_iGEM">
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<center><a href="http://www2.clustrmaps.com/counter/maps.php?url=https://2010.igem.org/Team:Freiburg_Bioware" id="clustrMapsLink"><img src="http://www2.clustrmaps.com/counter/index2.php?url=https://2010.igem.org/Team:Freiburg_Bioware" style="border:0px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com';" />
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<center><small>primary team contact: Kristian M. Müller; <a href="mailto:Kristian@syntbio.net">e-mail: Kristian@syntbio.net</a></small></center>
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Latest revision as of 15:23, 14 January 2011

Gene delivery using viral vectors holds great promise for the treatment of acquired and inherited diseases. The human Adeno-Associated Virus (AAV) is a small, non-pathogenic, single-stranded DNA virus gaining increasing attention being both versatile and effective. Taking current knowledge into account, we generated a recombinant, modularized, BioBrick-compatible AAV ‘Virus Construction Kit’. We provide parts for modified capsid proteins, targeting modules, tumor-specific promoters, and prodrug-activating enzymes as well as readily assembled vectors for gene delivery and production of non-replicative virus particles. The viral tropism is altered by N-terminal fusion or by loop replacement of the capsid proteins. Functionality of viruses constructed from our kit was demonstrated by fluorescent protein expression in infected cells and by prodrug-induced killing of tumor cells upon viral delivery of a thymidine kinase. Incorporating multiple layers of safety, we provide a general tool to the growing field of personalized medicine and demonstrate its use in tumor therapy.

Project Results

Team Freiburg presents a functional, modular Virus Construction Kit for specifically targeting and killing tumor cells. [more]

Structural engineering of the three dimensional viral capsid by introducing four distinct targeting sites led to successful differential transduction of tumor cells. [more]

Efficient gene-directed enzyme prodrug activation was demonstrated by BioBrick-compatible constructs introduced into tumor cells. [more]

Virus Construction Kit - The Manual

Build your own therapeutical virus – 150 pages of knowledge. [more]

Statistics - In a Minute

  • What happened in the lab during iGEM?

  • Longest Wikipage

    Longest Wikipage:
    with 222,795 bytes

  • Number of BioBricks

    Number of Biobricks:
    116

  • Number of ordered Oligo-Nucleotides

    Number of Ordered Oligos:
    193

  • Minipreps total

    Mini-Preps total:
    1085

  • Midipreps total

    Midi-Preps total:
    106

  • Highest Prep concentration (Midi)

    Highest Midi-Prep Concentration:
    5045,6 ng/µL

  • Number of Glycerolstocks

    Number of Glycerolstocks:
    763

  • Number of Team members

    Number of Team members:
    15

  • Days in the lab

    Days in the lab:
    170

  • Longest Labday

    Longest Labday:
    18h

  • Coffee consumed during iGEM

    Coffee Consumed During iGEM:
    2238 Cups

BioBricks

Modularization and modification of the viral capsids for retargeting approaches and directed gene delivery of suicide genes resulted in many different BioBricks.
Click here to explore them. [more]

Biosafety

Aspects of biological safety were considered throughout and a risk assessment and security profile of the Adeno-associated Virus were created. Read more about Biosafety issues. [more]

Notebook

From DNA to virus particle to cell culture?
Yes! Details on cloning, cell culture, flow cytometry analyses, quantifications by ELISA or Western Blot, cytotoxicity assays, time resolved microscopy ... [more]

Team

Fun and Science at the same time?
Yes! The iGEM team Freiburg did not only share the lab bench, but as well some nice days canoeing and initiated the photo contest Cuckoo Clock Competition. [more]


Sponsors

Uni Potsdam Uni Freiburg Bioss kuk.lab daad frias Roche Qiagen Starlab peqlab Eppendorf Gilson Fermentas aigilent Avidity Hiss Biozym GATC Geneart ATG purimex dkfz Mathworks


Locations of visitors to this page

primary team contact: Kristian M. Müller; e-mail: Kristian@syntbio.net