Team:Freiburg Bioware
From 2010.igem.org
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<html> | <html> | ||
- | <div class="virus"><img src=" | + | <div class="virus"><img src="https://static.igem.org/mediawiki/2010/2/24/Freiburg10_rot250trans.gif" title="virus (3.5MB)" id="animated_virus"/></div> |
<div class="div_home"> | <div class="div_home"> | ||
<p> | <p> | ||
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</div> | </div> | ||
- | <!---Box on the upper left: Project | + | <!---Box on the upper left: Project Results---> |
<div class="box_home"> | <div class="box_home"> | ||
<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project"><img class="right" src="https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png" id="ccc" /> | <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project"><img class="right" src="https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png" id="ccc" /> | ||
</a> | </a> | ||
- | <h2>Project Results</h2> | + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results"><h2>Project Results</h2></a> |
- | <p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#highlights"><h5>Highlights: </h5></a> | + | <p><!--<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#highlights"><h5>Highlights: </h5></a>--> |
- | + | Team Freiburg presents a functional, modular Virus Construction Kit for specifically targeting and killing tumor cells. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#highlights"><span class="bold">[more]</span></a></p> | |
- | <p><a href="https://2010.igem.org/Team:Freiburg_Bioware/ | + | <p><!--<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#highlights"><span class="bold"><h5>Heading:</h5></span></a>--> |
- | + | Structural engineering of the three dimensional viral capsid by introducing four distinct targeting sites led to successful differential transduction of tumor cells. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#targeting"><span class="bold">[more]</span></a></p> | |
- | <p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><span class="bold"><h5>Head-Line:</h5></span></a> | + | <p><!--<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><span class="bold"><h5>Head-Line:</h5></span></a>--> |
- | + | Efficient gene-directed enzyme prodrug activation was demonstrated by BioBrick-compatible constructs introduced into tumor cells. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#arming"><span class="bold">[more]</span></a></p> | |
</div> | </div> | ||
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<div class="box_long"> | <div class="box_long"> | ||
- | <img class="right" src=" | + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"> |
- | <h2>Virus Construction Kit - The Manual</h2> | + | <img class="right" src="https://static.igem.org/mediawiki/2010/f/fb/Freiburg10_Manual_Logo_small.png" id="ccc" /></a> |
- | <p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><h5>Heading:</h5></a> | + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><h2>Virus Construction Kit - The Manual</h2></a> |
- | + | <p><!--<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><h5>Heading:</h5></a>--> | |
+ | Build your own therapeutical virus – 150 pages of knowledge. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><span class="bold">[more]</span></a></p> | ||
</div> | </div> | ||
<!--- News Ticker ---> | <!--- News Ticker ---> | ||
- | <div class="box_long"> | + | <div class="box_long box_long_news news_ticker"> |
- | <h2>Statistics</h2> | + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Statistics" style="z-index:20;position:relative;"><h2>Statistics - In a Minute</h2></a> |
<ul id="news" class="newsticker"> | <ul id="news" class="newsticker"> | ||
- | <li><div class="news"><img class="right" src="https://static.igem.org/mediawiki/2010/0/ | + | <li><div class="news"><img class="right" src="https://static.igem.org/mediawiki/2010/0/0b/Freiburg10_Number_of_Plasmids.png" id="ccc" /><p> |
- | What happened in the | + | What happened in the lab during iGEM? </p> |
</div></li> | </div></li> | ||
- | + | <li><div class="news"><img | |
- | + | src="https://static.igem.org/mediawiki/2010/a/a5/Freiburg10_Longest_Wikipage.png" | |
- | + | alt="Longest Wikipage" width="368"><p>Longest Wikipage:<br />with 222,795 bytes</p></div> | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</li> | </li> | ||
- | <li><div class="news">Number of | + | <li><div class="news"><img src="https://static.igem.org/mediawiki/2010/1/10/Freiburg10_Number_of_Biobricks.png" |
- | <li><div class="news"> | + | alt="Number of BioBricks" width="368"><p>Number of Biobricks:<br /> 116</p></div></li> |
- | <li><div class="news"> | + | <li><div class="news"><img heigth="100px" src="https://static.igem.org/mediawiki/2010/3/3f/Freiburg10_Number_of_ordered_Oligo-Nucleotides.png" |
- | li><div class="news"> | + | alt="Number of ordered Oligo-Nucleotides" width="184"><p>Number of Ordered Oligos:<br /> 193</p></div></li> |
- | + | <li><div class="news"><img | |
- | + | src="https://static.igem.org/mediawiki/2010/f/f3/Freiburg10_Minipreps_total.png" | |
+ | alt="Minipreps total" width="184"><p>Mini-Preps total:<br /> 1085</p></div></li> | ||
+ | <li><div class="news"><img | ||
+ | src="https://static.igem.org/mediawiki/2010/f/f7/Freiburg10_Midipreps_total.png" | ||
+ | alt="Midipreps total" width="368"><p>Midi-Preps total:<br /> 106</p></div></li> | ||
+ | <li><div class="news"><img | ||
+ | src="https://static.igem.org/mediawiki/2010/d/d6/Freiburg10_Highest_Prep_concentration.png" | ||
+ | alt="Highest Prep concentration (Midi)" width="368"><p>Highest Midi-Prep Concentration:<br />5045,6 ng/µL </p></div></li> | ||
+ | <li><div class="news"><img | ||
+ | src="https://static.igem.org/mediawiki/2010/a/af/Freiburg10_Number_of_Glyzerolstocks.png" | ||
+ | alt="Number of Glycerolstocks" width="368"><p>Number of Glycerolstocks:<br /> 763 </p></div></li> | ||
+ | <li><div class="news"><img | ||
+ | src="https://static.igem.org/mediawiki/2010/6/60/Freiburg10_Number_of_Team_members.png" | ||
+ | alt="Number of Team members" width="368"><p>Number of Team members:<br /> 15 </p></div></li> | ||
+ | <li><div class="news"><img | ||
+ | src="https://static.igem.org/mediawiki/2010/0/03/Freiburg10_Days_in_the_lab.png" | ||
+ | alt="Days in the lab" width="368"><p>Days in the lab:<br /> 170 </p></div></li> | ||
+ | <li><div class="news"><img | ||
+ | src="https://static.igem.org/mediawiki/2010/d/d1/Freiburg10_Longest_Labday.png" | ||
+ | alt="Longest Labday" width="368"><p>Longest Labday:<br />18h </p></div></</li> | ||
+ | <li><div class="news"><img height="100px" | ||
+ | src="https://static.igem.org/mediawiki/2010/b/b5/Freiburg10_Coffee_consumed_during_iGEM.png" | ||
+ | alt="Coffee consumed during iGEM" width="184"><p>Coffee Consumed During iGEM:<br /> 2238 Cups</p></div></li> | ||
+ | <li><div class="news"><img heigth="100px"class="right" src="https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png" id="ccc" /><p><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Statistics"><h5>!Freiburg Bioware Statistics!</h5></a></p></div></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
+ | <div class="clearfix"></div> | ||
- | + | <div height="290px"> | |
<!---Box on the upper right: BioBricks---> | <!---Box on the upper right: BioBricks---> | ||
<div class="box_home_small"> | <div class="box_home_small"> | ||
- | <a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><img class="right" src="https://static.igem.org/mediawiki/2010/ | + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><img class="right" src="https://static.igem.org/mediawiki/2010/d/d1/Freiburg_10_BioBrick_icon_small.png" id="ccc" /></a> |
<a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><h2>BioBricks</h2></a> | <a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><h2>BioBricks</h2></a> | ||
- | <p class="standard"> | + | <p class="standard">Modularization and modification of the viral capsids for retargeting approaches and directed gene delivery of suicide genes resulted in many different BioBricks.<br> Click here to explore them. <a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><span class="bold">[more]</a></span></p> |
</div> | </div> | ||
<!---Box on the lower left: Biosafety---> | <!---Box on the lower left: Biosafety---> | ||
<div class="box_home_small"> | <div class="box_home_small"> | ||
- | <a href="https://2010.igem.org/Team:Freiburg_Bioware/ | + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/Safety"><img class="right" src="https://static.igem.org/mediawiki/2010/f/f9/Freiburg10_Danger_Virus_small.png" id="ccc" /></a> |
- | <a href="https://2010.igem.org/Team:Freiburg_Bioware/ | + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/Safety"><h2>Biosafety</h2></a> |
- | <p class="standard"> | + | <p class="standard">Aspects of biological safety were considered throughout and a risk assessment and security profile of the Adeno-associated Virus were created. Read more about Biosafety issues. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Safety"><span class="bold">[more]</span></a></p> |
- | + | ||
</div> | </div> | ||
<!---Box on the lower right: Notebook---> | <!---Box on the lower right: Notebook---> | ||
- | <a href="https://2010.igem.org/Team:Freiburg_Bioware/ | + | <div class="box_home_small"> |
- | <img class="right" src=" | + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook"> |
- | <a href="https://2010.igem.org/Team:Freiburg_Bioware/ | + | <img class="right" src="https://static.igem.org/mediawiki/2010/9/95/Freiburg_10_Notebook_image_small.png" id="ccc" /></a> |
+ | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook"> | ||
<h2>Notebook</h2></a> | <h2>Notebook</h2></a> | ||
- | <p><a href="https://2010.igem.org/Team:Freiburg_Bioware/ | + | <p> |
- | + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook"><span class="bold">From DNA to virus particle to cell culture? </span></a><br /> | |
- | + | Yes! Details on cloning, cell culture, flow cytometry analyses, quantifications by ELISA or Western Blot, cytotoxicity assays, time resolved microscopy ... <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Methods"><span class="bold">[more]</span></a></p> | |
</div> | </div> | ||
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<!---Box on the lower right: Team---> | <!---Box on the lower right: Team---> | ||
<div class="box_home_small"> | <div class="box_home_small"> | ||
- | <img class="right" src=" | + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Team_2010"> |
- | <a href="https://2010.igem.org/Team:Freiburg_Bioware/Team"> | + | <img class="right" src="https://static.igem.org/mediawiki/2010/b/bd/Freiburg10_Virus_Logo_Small_small.png" id="ccc" /></a> |
+ | <a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Team_2010"> | ||
<h2>Team</h2></a> | <h2>Team</h2></a> | ||
- | <p><a href="https://2010.igem.org/Team:Freiburg_Bioware/ | + | <p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Team_2010"><span class="bold">Fun and Science at the same time?</span></a></a><br /> |
- | + | Yes! The iGEM team Freiburg did not only share the lab bench, but as well some nice days <a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Photo_Gallery"><span class="bold">canoeing</span></a> and initiated the photo contest Cuckoo Clock Competition. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Cuckoo_Clock"><span class="bold">[more]</span></a></p> | |
- | + | </div> | |
- | </div><div class="clearfix"></div> | + | </div> |
- | < | + | <div class="clearfix"></div> |
- | + | <br /> | |
<h1>Sponsors</h1> | <h1>Sponsors</h1> | ||
- | <div class="map"> | + | |
+ | <div class="map" height="1169px"> | ||
<map name="Sponsoren_iGEM" id="Sponsoren_iGEM"> | <map name="Sponsoren_iGEM" id="Sponsoren_iGEM"> | ||
<area shape="circle" coords="705,369,65" href="http://www.uni-potsdam.de/" target="_blank" alt="Uni Potsdam" title="Uni Potsdam" /> | <area shape="circle" coords="705,369,65" href="http://www.uni-potsdam.de/" target="_blank" alt="Uni Potsdam" title="Uni Potsdam" /> | ||
<area shape="circle" coords="156,138,121" href="http://www.uni-freiburg.de/start-en.html?set_language=en" target="_blank" alt="Uni Freiburg" title="Uni Freiburg" /> | <area shape="circle" coords="156,138,121" href="http://www.uni-freiburg.de/start-en.html?set_language=en" target="_blank" alt="Uni Freiburg" title="Uni Freiburg" /> | ||
<area shape="rect" coords="304,8,815,269" href="http://www.bioss.uni-freiburg.de/cms/index.php#" target="_blank" alt="Bioss" title="Bioss" /> | <area shape="rect" coords="304,8,815,269" href="http://www.bioss.uni-freiburg.de/cms/index.php#" target="_blank" alt="Bioss" title="Bioss" /> | ||
- | <area shape="rect" coords="501,311,611,434" href="http://www. | + | <area shape="rect" coords="501,311,611,434" href="http://www.syntbio.net/" target="_blank" alt="kuk.lab" title="kuk.lab" /> |
- | <area shape="rect" coords="274,329,480,412" href=" | + | <area shape="rect" coords="274,329,480,412" href="http://www.daad.de/en/index.html" alt="daad" target="_blank" title="daad" /> |
- | <area shape="rect" coords="67,324,238,419" href=" | + | <area shape="rect" coords="67,324,238,419" href="http://www.frias.uni-freiburg.de/" alt="frias" target="_blank" title="frias" /> |
- | <area shape="rect" coords="95, | + | <area shape="rect" coords="95,534,376,686" href="http://www.roche.com/index.htm" target="_blank" alt="Roche" title="Roche" /> |
<area shape="rect" coords="510,458,677,606" href="http://www.qiagen.com/default_qs.aspx" target="_blank" alt="Qiagen" title="Qiagen" /> | <area shape="rect" coords="510,458,677,606" href="http://www.qiagen.com/default_qs.aspx" target="_blank" alt="Qiagen" title="Qiagen" /> | ||
<area shape="rect" coords="477,671,708,752" href="http://www.starlab.de/int/?l=2" target="_blank" alt="Starlab" title="Starlab" /> | <area shape="rect" coords="477,671,708,752" href="http://www.starlab.de/int/?l=2" target="_blank" alt="Starlab" title="Starlab" /> | ||
<area shape="rect" coords="551,799,787,861" href="http://www.peqlab.com" target="_blank" alt="peqlab" title="peqlab" /> | <area shape="rect" coords="551,799,787,861" href="http://www.peqlab.com" target="_blank" alt="peqlab" title="peqlab" /> | ||
<area shape="rect" coords="291,801,494,859" href="http://www.eppendorf.com/int/?l=1&action=start" target="_blank" alt="Eppendorf" title="Eppendorf" /> | <area shape="rect" coords="291,801,494,859" href="http://www.eppendorf.com/int/?l=1&action=start" target="_blank" alt="Eppendorf" title="Eppendorf" /> | ||
- | |||
<area shape="rect" coords="43,801,226,859" href="http://www.gilson.com/en/" target="_blank" alt="Gilson" title="Gilson" /> | <area shape="rect" coords="43,801,226,859" href="http://www.gilson.com/en/" target="_blank" alt="Gilson" title="Gilson" /> | ||
<area shape="rect" coords="44,872,218,936" href="http://www.fermentas.de/index.php?&language=en" target="_blank" alt="Fermentas" title="Fermentas" /> | <area shape="rect" coords="44,872,218,936" href="http://www.fermentas.de/index.php?&language=en" target="_blank" alt="Fermentas" title="Fermentas" /> | ||
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<area shape="rect" coords="296,886,540,948" href="http://www.geneart.com/" target="_blank" alt="Geneart" title="Geneart" /> | <area shape="rect" coords="296,886,540,948" href="http://www.geneart.com/" target="_blank" alt="Geneart" title="Geneart" /> | ||
<area shape="rect" coords="495,1070,808,1151" href="http://www.atg-biosynthetics.com/" target="_blank" alt="ATG" title="ATG" /> | <area shape="rect" coords="495,1070,808,1151" href="http://www.atg-biosynthetics.com/" target="_blank" alt="ATG" title="ATG" /> | ||
- | <area shape="rect" coords="242,1086,469,1154" href=" | + | <area shape="rect" coords="242,1086,469,1154" href="http://www.purimex.com/" alt="purimex" target="_blank" title="purimex" /> |
- | <area shape="rect" coords="45,1084,219,1154" href=" | + | <area shape="rect" coords="45,1084,219,1154" href="http://www.dkfz.de/index.html" alt="dkfz" target="_blank" title="dkfz" /> |
<area shape="rect" coords="46,1021,264,1075" href="http://www.mathworks.com" target="_blank" alt="Mathworks" title="Mathworks" /> | <area shape="rect" coords="46,1021,264,1075" href="http://www.mathworks.com" target="_blank" alt="Mathworks" title="Mathworks" /> | ||
</map> | </map> | ||
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<center><a href="http://www2.clustrmaps.com/counter/maps.php?url=https://2010.igem.org/Team:Freiburg_Bioware" id="clustrMapsLink"><img src="http://www2.clustrmaps.com/counter/index2.php?url=https://2010.igem.org/Team:Freiburg_Bioware" style="border:0px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com';" /> | <center><a href="http://www2.clustrmaps.com/counter/maps.php?url=https://2010.igem.org/Team:Freiburg_Bioware" id="clustrMapsLink"><img src="http://www2.clustrmaps.com/counter/index2.php?url=https://2010.igem.org/Team:Freiburg_Bioware" style="border:0px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com';" /> | ||
</a></center> | </a></center> | ||
+ | <br /> | ||
+ | <center><small>primary team contact: Kristian M. Müller; <a href="mailto:Kristian@syntbio.net">e-mail: Kristian@syntbio.net</a></small></center> | ||
</html> | </html> | ||
{{:Team:Freiburg_Bioware/Footer}} | {{:Team:Freiburg_Bioware/Footer}} |
Latest revision as of 15:23, 14 January 2011
![virus (3.5MB)](https://static.igem.org/mediawiki/2010/2/24/Freiburg10_rot250trans.gif)
Gene delivery using viral vectors holds great promise for the treatment of acquired and inherited diseases. The human Adeno-Associated Virus (AAV) is a small, non-pathogenic, single-stranded DNA virus gaining increasing attention being both versatile and effective. Taking current knowledge into account, we generated a recombinant, modularized, BioBrick-compatible AAV ‘Virus Construction Kit’. We provide parts for modified capsid proteins, targeting modules, tumor-specific promoters, and prodrug-activating enzymes as well as readily assembled vectors for gene delivery and production of non-replicative virus particles. The viral tropism is altered by N-terminal fusion or by loop replacement of the capsid proteins. Functionality of viruses constructed from our kit was demonstrated by fluorescent protein expression in infected cells and by prodrug-induced killing of tumor cells upon viral delivery of a thymidine kinase. Incorporating multiple layers of safety, we provide a general tool to the growing field of personalized medicine and demonstrate its use in tumor therapy.
![](https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png)
Project Results
Team Freiburg presents a functional, modular Virus Construction Kit for specifically targeting and killing tumor cells. [more]
Structural engineering of the three dimensional viral capsid by introducing four distinct targeting sites led to successful differential transduction of tumor cells. [more]
Efficient gene-directed enzyme prodrug activation was demonstrated by BioBrick-compatible constructs introduced into tumor cells. [more]
![](https://static.igem.org/mediawiki/2010/f/fb/Freiburg10_Manual_Logo_small.png)
Virus Construction Kit - The Manual
Build your own therapeutical virus – 150 pages of knowledge. [more]
Statistics - In a Minute
What happened in the lab during iGEM?
Longest Wikipage:
with 222,795 bytesNumber of Biobricks:
116Number of Ordered Oligos:
193Mini-Preps total:
1085Midi-Preps total:
106Highest Midi-Prep Concentration:
5045,6 ng/µLNumber of Glycerolstocks:
763Number of Team members:
15Days in the lab:
170Longest Labday:
18hCoffee Consumed During iGEM:
2238 Cups
![](https://static.igem.org/mediawiki/2010/d/d1/Freiburg_10_BioBrick_icon_small.png)
BioBricks
Modularization and modification of the viral capsids for retargeting approaches and directed gene delivery of suicide genes resulted in many different BioBricks.
Click here to explore them. [more]
![](https://static.igem.org/mediawiki/2010/f/f9/Freiburg10_Danger_Virus_small.png)
Biosafety
Aspects of biological safety were considered throughout and a risk assessment and security profile of the Adeno-associated Virus were created. Read more about Biosafety issues. [more]
![](https://static.igem.org/mediawiki/2010/9/95/Freiburg_10_Notebook_image_small.png)
Notebook
From DNA to virus particle to cell culture?
Yes! Details on cloning, cell culture, flow cytometry analyses, quantifications by ELISA or Western Blot, cytotoxicity assays, time resolved microscopy ... [more]
![](https://static.igem.org/mediawiki/2010/b/bd/Freiburg10_Virus_Logo_Small_small.png)
Team
Fun and Science at the same time?
Yes! The iGEM team Freiburg did not only share the lab bench, but as well some nice days canoeing and initiated the photo contest Cuckoo Clock Competition. [more]
Sponsors
![](https://static.igem.org/mediawiki/2010/4/49/Freiburg10_Sponsoren_Image_Map.gif)